Yerel entomopatojen fungus Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae)’nın Helicoverpa armigera (Hübner, 1808) (Lepidoptera: Noctuidae) üzerindeki etkinliği

Öz

Helicoverpa armigera (Hübner, 1808) (Lepidoptera: Noctuidae), sentetik insektisitlere karşı direnç geliştirmiş önemli bir tarımsal zararlıdır. Bu çalışmada, entomopatojenik fungus Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae)’ın H. armigera’nın altı larva dönemine karşı etkinliği 2024 yılında Bolu Abant Izzet Baysal Üniversitesi, Biyolojik Mücadele Laboratuvarı’nda incelenmiştir. Üç farklı konidiyal konsantrasyon (1×106, 1×10⁷ ve 1×10⁸ konidi ml⁻¹) kullanılarak ölüm oranları değerlendirilmiştir. Uygulama sonrası günler, doz ve larva dönemleri göz önünde bulundurularak, B. bassiana’nın farklı spor konsantrasyonlarının etkileri analiz edilmiştir. Probit analizi ile öldürücü konsantrasyonlar (LC₅₀ ve LC₉₀) ve öldürücü zaman değerleri (LT₅₀ ve LT₉₀) hesaplanmıştır. Ölüm oranlarının, artan dozlarla yükseldiği ve özellikle genç larva dönemlerinde belirgin olup, özellikle birinci larva döneminde 1×10⁸ konidi ml⁻¹ konsantrasyonunda, LT₅₀ ve LT₉₀ değerleri sırasıyla 4,05 ve 8,10 gün olarak kaydedilmiştir. Daha ileri dönemlerde ise ölüm oranlarının düştüğü tespit edilmiştir. LC₅₀ ve LC₉₀ değerleri, artan konsantrasyonlarla azalarak birinci ve ikinci dönem larvalarda tüm dozlarda %100 ölüm sağlamıştır. Üçüncü dönem larvalar doz-bağımlı bir tepki göstermiş ve LC₅₀ ile LC₉₀ değerleri sırasıyla 6.88×10⁸ ve 1.94×10⁴ konidi ml⁻¹ olarak bulunmuştur. Daha yüksek spor konsantrasyonları, LT₅₀ ve LT₉₀ sürelerini kısaltarak özellikle genç larvalarda ölüm oranlarını artırmıştır. Bu bulgular, B. bassiana’nın genç larva dönemlerine karşı etkinliğini, etkili biyolojik mücadele için uygulama zamanlamasının önemini ve entegre zararlı yönetimi stratejilerinde potansiyel rolünü vurgulamaktadır.

Anahtar Kelimeler

• Beauveria bassiana
• biyolojik mücadele
• biyopestisit
• entomopatojen fungus
• Helicoverpa armigera

Teşekkür

The author would like to thank Prof. Dr. Göksel Özer (Bolu Abant Izzet Baysal University, Türkiye) for their help in the identification of the entomopathogenic fungus and prepare fungal suspensions.

Efficacy of native entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) against Helicoverpa armigera (Hübner, 1808) (Lepidoptera: Noctuidae)

Öz

Helicoverpa armigera (Hübner, 1808) (Lepidoptera: Noctuidae) is a significant agricultural pest with resistance to conventional synthetic insecticides. The present study, conducted in 2024 at Bolu Abant Izzet Baysal University, Biological Control Laboratory, investigated the pathogenicity of Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) against six larval stages of H. armigera. Three conidial concentrations (1×10⁶, 1×10⁷, and 1×10⁸ conidia ml⁻¹) were evaluated. This study analyzed the effects of varying spore concentrations of B. bassiana on the mortality of H. armigera larvae, considering days post-application, dose, and larval stage. Lethal concentrations (LC50 and LC90) and lethal time values (LT50 and LT90) were calculated using probit analysis. Mortality increased with dose and was highest in early instars, particularly at 1×10⁸ conidia ml⁻¹, where LT₅₀ and LT₉₀ values for first instars were 4.05 and 8.10 days, respectively. Older instars exhibited lower mortality rates. LC₅₀ and LC₉₀ decreased with increasing concentrations, achieving 100% mortality in the first and second instars across all doses. Third instars displayed dose-dependent mortality, with LC₅₀ and LC₉₀ values of 6.88×10⁸ and 1.94×10⁴ conidia ml⁻¹, respectively. Higher spore concentrations reduced LT₅₀ and LT₉₀, enhancing mortality rates in younger larvae. These findings underscore the efficacy of B. bassiana against early larval stages, emphasizing the importance of application timing for effective biological control and its potential role in integrated pest management strategies.

Anahtar Kelimeler

• Beauveria bassiana
• biological control
• biopesticide
• entomopathogenic fungus
• Helicoverpa armigera

Teşekkür

The author would like to thank Prof. Dr. Göksel Özer (Bolu Abant Izzet Baysal University, Türkiye) for their help in the identification of the entomopathogenic fungus and prepare fungal suspensions.

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