Bazı bı̇yopestı̇sı̇tlerı̇n domateste zararlı kök-ur nematodu Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae) üzerine etkisi̇

Öz

Son yıllarda, biyopestisitler bitki paraziti nematodların (BPN) kontrolü için yaygın olarak araştırılmaktadır. Bu çalışmada Bacillus bazlı biyopestisitlerin kök-ur nematodu Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae) üzerindeki etkileri incelenmiştir. Çalışma, 2023 yılında Bolu Abant İzzet Baysal Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Nematoloji Laboratuvarı ve iklim odasında yürütülmüştür. Çalışmada Bacillus subtilis (Biyopestisit-I), Bacillus licheniformis suşu RTI184 (Biyopestisit-II) ve Paecilomyces lilacinus suşu 251'in (Biyopestisit-III) etkinliği, kimyasal nematisitler ve uygulama içermeyen bir kontrol ile karşılaştırılmıştır. Uygulamalar farklı zamanlarda gerçekleştirilmiştir: (A) dikimden 5-7 gün önce, (B) dikim sırasında daldırma yoluyla, (C) dikimden hemen sonra ve (D) dikimden 14 gün sonra (DAT). En düşük gal oluşumu Biyopestisit-III + Nematisit-II (CD) uygulamasında (0,40±0,24), ardından Nematisit-I (CD) (3,40±0,24) ve Biyopestisit-I (BD) (4,20±0,37) uygulamalarında gerçekleşmiş, en yüksek ise Biyopestisit-I (CD) (5,00±0,31) uygulamasında gözlenmiştir. İkinci dönem larva sayıları Biyopestisit-III + Nematisit-II (CD) (%99,76), Nematisit-I (CD) (%70,29) ve Biyopestisit-I (BD) (%57,36) ile önemli ölçüde azalmıştır. Sonuçlar, Bacillus bazlı biyopestisitlerin kök-ur nematodu zararını azaltmada etkili olduğunu ve domates bitkilerinde M. incognita'nın mücadelesinde kullanılabileceğini göstermektedir.

Anahtar Kelimeler

• Bacillus licheniformis RTI184 suşu
• Bacillus subtilis
• biyopestisit
• nematisit
• kök-ur nematodu

The effect of some biopesticides on the root-knot nematode, Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae) damaging tomato plants

Abstract

In recent years, biopesticides have been widely investigated for the control of plant parasitic nematodes (PPNs). In this study, the effects of Bacillus-based biopesticides on the root-knot nematode (Meloidogyne incognita) (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae) were investigated. The study was conducted at the Nematology Laboratory and Growth Room of the Department of Plant Protection, Faculty of Agriculture, Bolu Abant İzzet Baysal University in 2023. The efficacy of Bacillus subtilis (Biopesticide-I), Bacillus licheniformis strain RTI184 (Biopesticide-II) and Paecilomyces lilacinus strain 251 (Biopesticide-III) was compared with chemical nematicides and an untreated control. Treatments were applied at different times: (A) 5-7 days before transplanting, (B) at transplant by drench, (C) just after transplanting, and (D) 14 days after transplanting (DAT). The lowest gal formation was observed in Biopesticide-III + Nematicide-II (CD) treatment (0.40±0.24), followed by Nematicide-I (CD) (3.40±0.24) and Biopesticide-I (BD) (4.20±0.37), while the highest was observed in Biopesticide-I (CD) (5.00±0.31). The number of second-stage juveniles was significantly reduced by Biopesticide-III + Nematicide-II (CD) (99.76%), Nematicide-I (CD) (70.29%) and Biopesticide-I (BD) (57.36%). The results indicate that Bacillus-based biopesticides are effective in reducing root-knot nematode damage and can be used to control M. incognita in tomato plants.

Keywords

• Bacillus licheniformis strain RTI184
• Bacillus subtilis
• biopesticide
• nematicide
• root-knot nematode

Kaynakça

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