Determination of the efficacy of some entomopathogenic nematodes against Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) under laboratory conditions

Yıl 2016, , 0 - 0, 16.06.2016

Galip Kaşkavalcı , Sevgi Türköz

https://doi.org/10.16970/ted.92606

Cited By: 4

Öz

The tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) which was first detected in Izmir Province, Turkey in 2009 has spread quickly and has become the major pest in tomato producing areas. The efficacy of three different entomopathogenic nematode (EPN) species isolates (from e-nema^® GmbH Schwentinental, Germany), Heterorhabditis bacteriophora (Poinar), Steinernema carpocapsae (Weiser) and Steinernema feltiae (Filipjev) was investigated against T. absoluta during 2013-2014 under laboratory conditions. The EPNs were applied with different inoculation rates (1, 2, 5, 10, 15, 20, 25 and 40 infective juveniles per larva) for each species to the third instar larvae of T. absoluta outside the leaves. The mortality rates for H. bacteriophora, S. carpocapsae and S. feltiae were found between 21.2 - 74.2%, 28.8 - 99.4% and 17.5 - 95.2%, respectively. According to the results, S. carpocapsae and S. feltiae caused similar mortalities at the given inoculation rates while H. bacteriophora had lower efficacy compared to those two species. The values of LD₅₀ for H. bacteriophora, S. carpocapsae and S. feltiae were 21.67, 7.13 and 6.25 infective juveniles per larva, respectively. Based on these data, S. feltiae was the most efficient nematode species, and was then applied against the larva of T. absoluta inside the mines and pupae. However, S. feltiae only caused low mortality of larvae both inside mines (19%) and the pupae (7%). These results revealed that EPN have good potential for the control of T. absoluta larvae outside the leaves and should be studied further.

Anahtar Kelimeler

Efficacy, Heterorhabditis, Steinernema, tomato, Tuta absoluta

Kaynakça

• Abbott, W. S. 1925. A method of computing the effectiveness of an insecticide. Journal Economic Entomology, 18: 265-267.
• Anonymous, 2013. Tuta absoluta Information Network. (Web page:http://www.tutaabsoluta.com), (Date accessed: December 2013).
• Anonymous, 2016. ‘Agricultural Land by Provinces. (Web page: http://www.turkstat.gov.tr/PreTablo.do?alt_id=1001), (Date accessed: March 2016).
• Arthurs, S., K. M. Heinz & J. R. Prasifka, 2004. An analysis of using entomopathogenic nematodes against above-ground pests. Bulletin of Entomological Research, 94: 297–306.
• Batalla-Carrera, L., A. Morton & F. Garcia-del-Pino, 2010. Efficacy of entomopathogenic nematodes against the tomato leafminer Tuta absoluta in laboratory and greenhouse conditions. BioControl, 55: 523–530.
• Begley, J. W., 1990. ‘’Efficacy Against Insects in Habitats Others than Soil,215-231’’. In: Entomopathogenic Nematodes in Biological Control (Eds. R. Gaugler & H. K. Kaya). CRC Press, Boca Raton, FL.
• Brévault, T., S. Sylla, M. Diatte, G. Bernadas & K. Diarra, 2014. Tuta absoluta Meyrick (Lepidoptera: Gelechiidae): A New Threat to Tomato Production in Sub-Saharan Africa. African Entomology, 22 (2): 441-444.
• Desneux, N., E. Wajnberg, K. A. G. Wyckhuys, G. Burgio, S. Arpaia, C. A. Narvaez-Vasquez, J. Gonzalez-Cabrera, D.C. Ruescas, E. Tabone, J. Frandon, J. Pizzol, C. Poncet, T. Cabello & A. Urbaneja, 2010. Biological invasion of European tomato crops by Tuta absoluta: Ecology, geographic expansion and prospects for biological control. Journal of Pest Science, 83: 197-215.
• Dowds, B. C. & A. Peters, 2002. ‘’Virulence mechanisms, 79–98’’. In: Entomopathogenic Nematology (Ed. R. Gaugler). CABI Publishing, Oxon, UK.
• Ehlers, R.U., 1996. Current and future use of nematodes in biocontrol: practice and commercial aspects with regard to regulatory policy issues. Biocontrol Science and Technology, 6: 303-316.
• Erler, F., M. Can, M. Erdogan, A. O. Ates, & T. Pradier, 2010. New record of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) on greenhouse-grown 46 tomato in southwestern Turkey (Antalya). Journal of Entomological Science, 45: 392-393.
• Garcia-del-Pino, F., X. Alabern & A. Morton, 2013. Efficacy of soil treatments of entomopathogenic nematodes against the larvae, pupae and adults of Tuta absoluta and their interaction with the insecticides used against this insect. BioControl, 58: 723–731.
• Glazer, I., 1996. Survival mechanisms of entomopathogenic nematodes. Biocontrol Science and Technology, 6: 373-378.
• Gözel, U. & Ç. Güneş, 2013. Effect of entomopathogenic nematode species on the corn stalk borer (Sesamia cretica Led. Lepidoptera: Noctuidae) at different temperatures. Türkiye Entomoloji Dergisi-Turkish Journal of Entomology, 37 (1): 65-72.
• Gözel, Ç., U. Gözel & İ. Kasap, 2014. “Domates güvesi Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)’nin mücadelesinde entomopatojen nematodların kullanım olanakları, 319”. Türkiye V. Bitki Koruma Kongresi (3-5 Şubat 2014, Antalya) Bildirileri, XMAT Matbaa, 417 s.
• Gözel, Ç. & İ. Kasap, 2015. Efficacy of entomopathogenic nematodes against the Tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in tomato field. Türkiye Entomoloji Dergisi-Turkish Journal of Entomology, 39 (3): 229-237.
• Grewal, P. S., R. U. Ehlers & D. I. Shapiro-Ilan, 2005. Nematodes as Biocontrol Agents. CABI Publisher, Wallingford, UK, 505pp.
• Kaya, H. K. & R. Gaugler, 1993. Entomopathogenic nematodes. Annual Review of Entomology, 38: 181-206.
• Kaya, H. K. & S. P. Stock, 1997. “Techniques in Insect Nematology, 281–324”. In: Manual of Techniques in Insect Pathology, (Ed. L. A. Lacey). Academic Press, San Diego.
• Kılıc, T., 2010. First record of Tuta absoluta in Turkey. Phytoparasitica, 38: 243–244.
• Klein, M. G., 1990. “Efficacy against Soil-Inhabiting Insects Pest, 195–214”. In: Entomopathogenic Nematodes Biological Control, (Eds. R. Gaugler & H. K. Kaya). CRC Press, Boca Raton, FL.
• Lacey, L. A. & T. R. Unruh, 1998. Entomopathogenic nematodes for control of codling moth: Effect of nematode species, dosage, temperature, and humidity under laboratory and simulated field conditions. Biological Control, 13:190–197.
• Pfeiffer, D. G., R. Muniappan, D. Sall, P. Diatta, A. Diongue & E. O. Dieng, 2013. First record of Tuta absoluta (Lepidoptera: Gelechiidae) in Senegal. Florida Entomologist, 96: 661–662.
• Poinar, G. O. Jr., 1990. “Biology and taxonomy of Steinernematidae and Heterorhabditidae, 23-61”. In: Entomopathogenic Nematodes in Biological Control (Eds: R., Gaugler & H. K. Kaya). CRC Press, Boca Raton, FL.
• Potting, R., 2009. Pest risk analysis. Tuta absoluta, tomato leaf miner moth. Plant Protection Service of the Netherlands, 24.
• Salari, E., Karimi, J., Sadeghi-Nameghi, H. & Hosseini, M., 2014. Efficacy of two entomopathogenic nematodes Heterorhabditis bacteriophora and Steinernema carpocapsae for control of the leopard moth borer Zeuzera pyrina (Lepidoptera: Cossidae) larvae under laboratory conditions. Biocontrol Science and Technology, 25 (3): 260-275.
• Shapiro-Ilan, D. I., R. Han & C. Dolinksi, 2012. Entomopathogenic nematode production and application technology. Journal Nematology, 44 (2): 206–217.
• Spiridonov, S., E. Akhmedov & F. Belostotskaya, 1991. Proliferation of symbiotic bacteria in the intestinal vesicles of invasive larvae of Neoaplectana spp.(Nematoda, Steinernematidae). Helminthologia, 28: 141-142.
• Tomalak, M., S. Piggott & G. B. Jagdale, 2005. “Glasshouse Applications, 147–166”. In: Nematodes as Biocontrol Agents (Eds. P. S. Grewal, R. U. Ehlers & D. I. Shapiro-Ilan). CABI Publishing, Oxon.
• Urbaneja, A., R. Vercher, V. Navarro, F. Garcia Mari & J. L. Porcuna, 2007. La polilla del tomate, Tuta absoluta. Phytoma España, 194:16–23.
• Usda-Aphis, 2011. Federal import quarantine order for host materials of tomato leafminer, Tuta absoluta (Meyrick), (Web page: http://www.aphis.usda.gov/import_export/plants/plant_imports/federal_order/downloads/2011/Tuta%20absoluta5-5-2011.pdf), (Date accessed: April 2014).
• Webster, J. M., C. Chen & K. Hu, 2002. Bacterial metabolites. Entomopathogenic Nematology, 99-114.
• Williams, E. C. & K. F. A. Walters, 1999. Foliar application of entomopathogenic nematode Steinernema feltiae against leafminers on vegetables. Biocontrol Science and Technology, 10: 61–70.