Oxidative effects of boric acid on different developmental stages of Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae)

Abstract

Synthetic organic insecticides are widely used to combat agricultural pests. Boric acid has a great importance in pest management because it has less toxic effect on non-target organisms compared to other organic chemical insecticides. For this purpose, the fruit fly Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae) was reared from first stage larvae on an artificial diet containing boric acid at 10, 100, 200 or 300 mg/L to adult stage. The effect of boric acid on important oxidative stress indicators such as lipid peroxidation product of malondialdehyde contents (MDA) and protein oxidation products of protein carbonyl contents (PCO) and detoxification enzyme activity of glutathione S-transferase (GST) in the third stage larvae, pupae, adults and eggs of D. melanogaster were investigated. All boric acid concentrations significantly increased MDA content in third stage larva. When the adults from the larvae reared on 300 mg/L of dietary BA were also fed with high BA concentration for a 10-day period, MDA and PCO contents of male and female adults were considerably went up in comparison to control. MDA and PCO content in the eggs of these females were hugely increased. The rise in PCO content of the eggs was 31-fold relative to control. Our results indicate that BA feeding at high concentrations in all developmental stages of D. melanogaster is more effective on oxidative stress indicators and detoxification enzyme.

Keywords

• Boric acid,Drosophila melanogaster,oxidative stress

Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae)’in farklı gelişme dönemleri üzerine Borik asitin oksidatif etkileri

Öz

Tarımsal zararlılar ile mücadelede sentetik organik insektisitler yoğun olarak kullanılmaktadır. Borik asit organik kimyasal insektisitlere göre hedef olmayan organizmalara karşı daha düşük toksisiteye sahip olması nedeniyle önem taşımaktadır. Bu amaçla çalışmamızda meyve sineği Drosophila melanogaster Meigen,1830 (Diptera: Drosophilidae)'in birinci dönem larvaları borik asitin farklı konsantrasyonlarını (10, 100, 200 ve 300 mg/L) içeren yapay besinler ile yetiştirilmiştir. Drosophila melanogaster' in üçüncü dönem larva, pupa, ergin dönemleri ve yumurtalarında oksidatif stresin önemli indikatörleri olan lipid peroksidasyonu ürünü malondialdehid (MDA) ve protein oksidasyon ürünü protein karbonil miktarları (PCO) ile detoksifikasyon enzimi glutatyon-S-transferaz (GST) aktivitesi üzerine etkisi incelenmiştir. Borik asitin denenen konsantrasyonlarını içeren yapay besinler ile yetiştirilen D. melanogaster’ in üçüncü dönem larvalarının MDA miktarı önemli derecede artmıştır. Yüksek borik asit ile yetiştirilen erginlerin 10 gün süreyle 300 mg/L borik asit içeren besin ile beslenilmesi sonucunda kontrol besinine göre dişi ve erkek bireylerde MDA ve PCO miktarları önemli derecede artmıştır. Bu dişilerin yumurtalarındaki MDA, PCO miktarları ve GST aktivitesi önemli derecede artarken, PCO miktarındaki artış yaklaşık 31 katı oranında olmuştur. Sonuçlarımız, borik asitin yüksek konsantrasyonlarıyla beslenen D. melanogaster’in tüm gelişme dönemlerindeki oksidatif stres indikatörleri ve detoksifikasyon enzimi üzerine oldukça etkili olduğunu göstermiştir.

Anahtar Kelimeler

• Borik asit,Drosophila melanogaster,oksidatif stres

Kaynakça

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