Laboratory assessment for biological control of Tribolium confusum du Val., 1863 (Coleoptera: Tenebrionidae) by entomopathogenic fungi

Abstract

This research was carried out at the Plant Protection Department, Agricultural Faculty, Ataturk University (Erzurum, Turkey) in 2016. The objective of this study is to determine using as biological control agent against Tribolium confusum du Val., 1863 (Coleoptera: Tenebrionidae) adults of seven entomopathogenic fungal treatments, Beauveria bassiana, Paecilomyces farinosus, Isaria fumosorosea, Isaria farinosa, Lecanicillium muscarium (2 isolates) and an extract of L. muscarium, under laboratory conditions (25±1°C and 75±1% RH). Fungal isolates at two different concentrations (1x10⁵ and 1×10⁷ conidia/mL) were sprayed on the tested adult insects in Petri dishes.  The results demonstrated that the mortality rates of T. confusum adults treated with seven entomopathogenic fungi varied from 34.6 to 100% after 10 days of treatment. The entomopathogenic fungi isolates at both 1×10⁵ and 1×10⁷ conidia concentration caused in high mortality levels of T. confusum adults. In conclusion, it was observed that tested seven entomopathogenic fungi isolates might have a potential effect to biological control of this stored-product pest.

Keywords

• Biological control,entomopathogenic fungi,stored-product insect,Tribolium confusum

Tribolium confusum du Val., 1863 (Coleoptera: Tenebrionidae)’un biyolojik mücadelesinde entomopatojen fungusların kullanımının laboratuvar ortamında değerlendirilmesi

Öz

Bu çalışma, 2016 yılında Atatürk Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü (Erzurum, Türkiye)’nde yürütülmüştür. Çalışmanın amacı, yedi entomopatojen fungal (Beauveria bassiana, Paecilomyces farinosus, Isaria fumosorosea, I. farinosa, Lecanicillium muscarium (2 izolat) ve L. muscarium ekstraktı) izolatlarının, laboratuvar şartlarında (25±1°C and 75±1% RH). Tribolium confusum du Val., 1863 (Coleoptera: Tenebrionidae) erginlerine karşı biyolojik kontrol ajanı olarak kullanımlarını tespit etmektir. Fungus izolatları petri kaplarında test edilen ergin böceklere karşı iki farklı konsantrasyonda (1x10⁵ ve 1×10⁷ konidi/mL) sprey şekilde uygulanmıştır. Elde edilen sonuçlar, uygulamadan 10 gün sonra yedi entomopatojen fungus izolatı uygulaması ile T. confusum erginlerinin ölüm oranlarının %34.6’dan %100’e kadar değiştiğini göstermiştir. Entomopatojen fungus izolatları 1×10⁵ ve 1×10⁷ konsantrasyonlarında T. confusum erginlerinde yüksek seviyede ölüme neden olmuştur. Sonuç olarak, test edilen yedi fungal entomopatojenin depolanmış ürün zararlılarının biyolojik mücadelesi için potansiyel etkiye sahip olabileceği gözlemlenmiştir. 

Anahtar Kelimeler

• Biyolojik mücadele,entomopatojen funguslar,depolanmış ürün zararları,Tribolium confusum

Kaynakça

1. Aitken, A. D., 1975. Insect Travellers, I: Coleoptera. Technical Bulletin 31, H.M.S.O. London, 190 pp.
2. Akbar, W., J. C. Lord, J. R. Nechols & R. W. Howard, 2004. Diatomaceous earth increases the efficacy of Beauvera bassiana against Tribolium castaneum larvae and increase conidia attachment. Journal of Economic Entomology, 97 (2): 273-280.
3. Akello, J. T., T. Dubois, C. S. Gold, D. Coyne, J. Nakavuma & P. Paparu, 2007. Beauveria bassiana (Balsamo) Vuillemin as an endophyte in tissue culture banana (Musa spp.). Journal of Invertebrate Pathology, 96: 34–42.
4. Altieri, M. A., 1999. The ecological role of biodiversity in agroecosystems. Agriculture, Ecosystems & Environment, 74: 19–31.
5. APRD, 2016. Arthropod Pesticide Resistance Database, USA. (Web page: http://www.pesticideresistance.org/) (Date accessed: October 2016).
6. Arnold, A. E. & L. C. Lewis, 2005. “Ecology and evolution of fungal endophytes, and their roles against insects, 74-96”. In: Insect-Fungal Associations: Ecology and Evolution (Eds. F. E. Vega & M. Blackwell). Oxford University Press, New York, 352 pp.
7. Arthur, F. H. & J. L. Zettler, 1992. Malathion resistance in Tribolium confusum Duv. (Coleoptera: Tenebrionidae): correlating results from topical applications with residual mortality on treated surfaces. Journal of Stored Products Research, 28: 55-58.
8. Arthur, F. H., 1996. Grain protectants: Current status and prospects for the future. Journal of Stored Products Research, 32: 293–302.

9. Barra, P., L. Rosso, A. Nesci & M. Etcheverry, 2013. Isolation and identification of entomopathogenic fungi and their evaluation against Tribolium confusum, Sitophilus zeamais, and Rhyzopertha dominica in stored maize. Journal of Pest Science, 86 (2): 217–226.
10. Batta, Y. A., 2004. Control of the rice weevil (Sitophilus oryzae L. (Coleoptera: Curculionidae)) with various formulations of Metarhizium anisopliae. Crop Protection, 23 (2): 103-108.
11. Batta, Y. A., 2005. Control of the lesser grain borer Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) by treatments with residual formulations of Metarhizium anisopliae (Metschinkoff) Sorokin (Deuteromycotina: Hyphomycetes). Journal of Stored Products Research, 41: 221–229.
12. Bell, C. H. & S. M. Wilson, 1995. Phosphine tolerance and resistance in Trogoderma granarium Everts (Coleoptera: Dermetidae). Journal of Stored Products Research, 31: 199-205.
13. Bell, C. H., 2000. Fumigation in the 21st Century. Crop Protection, 19: 563-569.
14. Caddick, L., 2004. Search for methyl bromide and phosphine alternatives. Outlooks Pest Management, 6, 118-119.
15. Champ, B. R. & C. E. Dyte, 1976. Report of the FAO Global Survey of Pesticide Susceptibility of Stored Grain Pests. FAO, Rome, 297pp.
16. Dal Bello, G., S. Padin, C. Lopez Lastra & M. Fabrizio, 2001. Laboratory evaluation of chemical-biological control of the rice weevil (Sitophilus oryzae L.) in stored grains. Journal of Stored Products Research, 37: 77-84.
17. Fiedler, Z. & D. Sosnowska, 2007. Nematophagous fungus Paecilomyces lilacinus (Thom) Samson is also a biological agent for control of greenhouse insects and mite pests. BioControl, 25: 547-558.
18. Gómez-Vidal, S., L. V. Lopez-Llorca, H. B. Jansson & J. Salinas, 2006. Endophytic colonization of date palm (Phoenix dactylifera L.) leaves by entomopathogenic fungi. Micron, 37: 624–632.
19. Gurr, G. M., S. D. Wratten & J. M. Luna, 2003. Multi-function agricultural biodiversity: Pest management and other benefits. Basic and Applied Ecology, 4: 107-116.
20. Gurulingappa, P., P. A. McGee & G. Sword, 2010. Endophytic Lecanicillium lecanii and Beauveria bassiana reduce the survival and fecundity of Aphis gossypii following contact with conidia and secondary metabolites. Crop Protection, 30: 349-353.
21. Hodges, R. J., R. Robinson & D. R. Hall, 1996. Quinone contamination of de-husked rice by Tribolium castaneum (Herbst) (Coleoptera: Bostrichidae). Journal of Stored Products Research, 32: 31-37.
22. Isaka, M., P. Kittakoop, K. Kirtikara, N. L. Hywel-Jones & Y. Thebtaranonth, 2005. Bioactive substances from insect pathogenic fungi. Accounts of Chemical Research, 38: 813-823.
23. Jaber, L. R., 2015. Grapevine leaf tissue colonization by the fungal entomopathogen Beauveria bassiana s.l. and its effect against downy mildew. BioControl, 60: 103-112.
24. Jaronski, S. T., 2010. Ecological factors in the inundative use of fungal entomopathogens. BioControl, 55: 159-185.
25. Jones, K. D., 1994. Aspects of the Biology and Biological Control of the European Corn Borer in North Carolina. Ph.D. Thesis, Department of Entomology, North Carolina State University, 53 pp.
26. Kavallieratos, N. G., C. G. Athanassiou, M. P. Michalaki, Y. A. Batta, H. A. Rigatos, F. G. Pashalidou, G. N. Balotis, Z. Tomanovic & B. J. Vayias, 2006. Effect of the combined use of Metarhizium anisopliae (Metschnikoff) Sorokin and diatomaceous earth for the control of three stored-product beetle species. Crop Protection, 25 (10): 1087–1094.
27. Leckie, B. M., 2002. Effects of Beauveria bassiana Mycelia and Metabolites Incorporated into Synthetic Diet and Fed to Larval Helicoverpa zea, and Detection of Endophytic Beauveria bassiana in Tomato Plants Using PCR and ITS. M.S. Thesis, Department of Entomology, University of Tennessee, 24 pp.
28. Lord, J. C., 2001. Desiccant dusts synergize the effect of Beauveria bassiana (Hyphomycetes: Moniliales) on storedgrain beetles. Journal of Economic Entomology, 94 (2): 367–372.
29. Lord, J. C., 2005. Low humidity, moderate temperature and desiccant dust favor the efficacy of Beauveria bassiana (Hyphomycetes: Moniliales) for the lesser grain borer Rhyzopertha dominica (Coleoptera: Bruchidae). Biological Control, 34 (2): 180–186.
30. Madrid, F. J., N. D. Whit & S. R. Loschiavo, 1990. Insects in stored cereals, and their association with farming practices in southern Manitoba. The Canadian Entomologist, 122: 515–523.
31. Michalaki, M. P., C. G. Athanassiou, N. G. Kavallieratos, Y. A. Batta & J. N. Balotis, 2006. Effectiveness of Metarhizium anisopliae (Metschnikoff) Sorokin applied alone or in combination with diatomaceous earth against Tribolium confusum Jacquelin du Val: influence of temperature relative humidity and type of commodity. Crop Protection, 25 (5): 418–425.
32. Mills, K. A., 1983. Resistance to the fumigant hydrogen phosphine in some stored product insect species associated with repeated inadequate treatments. Mitteilungen der Deutschen Gesellschaft für allgemeine und angewandte Entomologie, 4: 98-101.
33. Monlar, I., D. M. Gibson & S. B. Krasnoff, 2010. Secondary metabolites from Entomopathogenic Hypocrealean Fungi. Natural Product Reports, 27 (9): 1241-1275.
34. Moore, D., J. C. Lord & S. M. Smith, 2000. “Pathogens, 193–227”. In: Alternatives to Pesticides in Stored-Product IPM (Eds. Bh. Subramanyam & D.W. Hagstrum). Kluwer Academic Publishers, 437 pp.
35. Ownley, B. H., R. M. Pereira, W. E. Klingeman, N. B. Quigley & B. M. Leckie, 2004. “Beauveria bassiana, a Dual Purpose Dordreecht Biocontrol Organism, with Activity Against Insect Pests and Plant Pathogens, 225-269”. In: Emerging Concepts in Plant Health Management (Eds. R.T. Lartey & A. J. Cesar). Research Signpost, India, 298 pp.
36. Padin, S. B., G. M. Dal Bello & M. Fabrizio, 2002. Grain losses caused by Tribolium castaneum, Sitophilus oryzae and Acanthoscelides obtectus in stored durum wheat and beans treated with Beauveria bassiana. Journal of Stored Products Research, 38: 69–74.
37. Padin, S. B., G. M. Dal Bello & A. L. Vasicek, 1997. Pathogenicity of Beauveria bassiana for adults of Tribolium castaneum (Col.: Tenebrionidae) in stored grains. Entomophaga, 42 (4): 569-574.
38. Posada, F., M. C. Aime, S. W. Peterson, S. A. Rehner & F. E. Vega, 2007. Inoculation of coffee plants with the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales). Mycological Research, 111: 749–758.
39. Rajendran, S., 2002. “Postharvest Pest Losses, 654-656”. In: Encyclopedia of Pest Management (Ed. D. Pimentel). Marcel Dekker, Inc, New York, 931 pp.
40. Rehner, S. A., 2005. “Phylogenetics of the insect pathogenic genus Beauveria, 3-27”. In: Insect-Fungal Associations-Ecology and Evolution (Ed. F. E. Vega & M. Blackwell), Oxford University Press, 352 pp.
41. Rice, W. C. & R. R. Cogburn, 1999. Activity of the entomopathogenic fungus Beauveria bassiana (Deuteromycota: Hyphomycetes) against three Coleopteran pests of stored grain. Journal of Economic Entomology, 92: 691-694.
42. Roberts, D. W. & R. A. Humber, 1981. “Entomogenous Fungi, 201–236”. In: Biology of Conidial Fungi, (Eds, G. T. Cole & B. Kendrick). Academic Press, New York, 484 pp.
43. Roberts, D. W. & R. J. St Leger, 2004. “Metarhizium spp., Cosmopolitan Insect–Pathogenic Fungi: Mycological Aspects. In: Advances in Applied Biology, (Eds. A. I. Laskin, J. W. Bennett & G. Gadd). Vol. 54, Academic Press, Elsevier, 386 pp.
44. Shaaya, E., M. Kostjukovski, J. Eilberg & C. Sukprakarn, 1997. Plant oils as fumigants and contact insecticides for the control of stored-product insects. Journal of Stored Products Research, 33: 7-15.
45. Taylor, R. W. D., 1989. Phosphine a major fumigant at risk. International Pest Control, 31: 10-14.
46. Tripathi, A. K., A. K. Singh & S. Upadhyay, 2009. Contact and fumigant toxicity of some common spices against the storage insects Callosobruchus maculatus (Coleoptera: Bruchidae) and Tribolium castaneum (Coleoptera: Tenebrionidae). International Journal of Tropical Insect Science, 29: 151-157.
47. Tscharntke, T., A. M. Klein, A. Kruess, I. Steffan-Dewenter & C. Thies, 2005. Landscape perspectives on agricultural intensification and biodiversity-ecosystem service management. Ecology Letters, 8: 857-874.
48. Vassilakos, T. N., C. G. Athanassiou, N. G. Kavallieratos & B. J. Vayias, 2006. Influence of temperature on the insecticidal effect of Beauveria bassiana in combination with diatomaceous earth against Rhyzopertha dominica and Sitophilus oryzae on stored wheat. Biological Control, 38: 270–281.
49. Via, S., 1999. Cannibalism facilitates the use of a novel environment in the flour beetle, Tribolium castaneum. Heredity, 82: 267-275.
50. Wagner, B. L. & L. C. Lewis, 2000. Colonization of corn, Zea mays, by the entomopathogenic fungus Beauveria bassiana. Applied and Environmental Microbiology, 66: 3468–3473.
51. Wakil, W., M. U. Ghazanfar & M. Yasin, 2014. Naturally occurring entomopathogenic fungi infecting stored grain insect species in Punjab, Pakistan. Journal of Insect Science, 14 (182): 1-7.
52. Weston, P. A. & P. L. Rattlingourd, 2000. Progeny production by Tribolium castaneum (Coleoptera: Tenebrionidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae) on maize previously infested by Sitotroga cerealla (Lepidoptera: Gelechiidae). Journal of Economic Entomology, 93: 533-536.
53. Zettler, J. L., 1991. Pesticide resistance in Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) from flour mills in the United States. Journal of Economic Entomology, 84: 763-767.
54. Zettler, J. L. & F. H. Arthur, 1997. Dose-response tests on red flour beetle and confused flour beetle (Coleoptera: Tenebrionidae) collected from flour mills in the United States. Journal of Economic Entomology, 90: 1157- 1162.