Farklı yaprak biti türlerinden izole edilen Buchnera aphidicola Munson et al., 1991 (Enterobacteriales: Enterobacteriaceae)’nın 16S rRNA’ya göre filogenetiği

Öz

Obligat simbiyont, Buchnera aphidicola Munson et al., 1991 (Enterobacteriales: Enterobacteriaceae), yaprak bitlerinin fizyolojik olaylarının sürdürülmesinde önemli bir rol oynar. Adana (Türkiye)’ dan 2017 yılında farklı bitki ve yüksekliklerden toplanan yedi yaprak biti türünde saptanan B. aphidicola genleri ile yaprakbiti türleri arasındaki filogenetik etkileşimi ortaya çıkarmak için analiz edilmiştir. Bu amaçla, 16S rRNA’nın gen bölgeleri kullanılmış ve filogenetik ağaç, neighbor-joining ile oluşturulmuştur. Buchrena aphidicola genleri ve aphid türleri arasında filogenetik ağaç ve haploid networke göre anlamlı bir korelasyon tespit edilmiştir. Solanum melongena L. toplanan Feke örneği, diğer Aphis gossypii Glover, 1877 (Hemiptera: Aphididae)’lerdeki B. aphidicola genlerinden çok yüksek bir boostrap değeri (99) ile ayrılmıştır. Myzus cinsindeki B. aphidicola genleri diğer cinslerden ayrı dallanmış ve Myzus cerasi (Fabricus, 1775) ve Myzus persicae Sulzer, 1776 arasındaki ayrım belirgindir. Buchera aphidicola konukçu yaprakbiti türüne özelleşmiş olsa da tür içinde elde edilen bazı nükleotid farklılıkları ilerde coğrafik bölgeye ya da bitkiye de özelleşmeye neden olabilir.

Anahtar Kelimeler

• Yaprak biti,genetik benzerlik,filogenetik,simbiyotik bakteri

Phylogenetics of Buchnera aphidicola Munson et al., 1991 (Enterobacteriales: Enterobacteriaceae) based on 16S rRNA amplified from seven aphid species

Abstract

The obligate symbiont, Buchnera aphidicola Munson et al., 1991 (Enterobacteriales: Enterobacteriaceae) is important for the physiological processes of aphids. Buchnera aphidicola genes detected in seven aphid species, collected in 2017 from different plants and altitudes in Adana Province, Turkey were analyzed to reveal phylogenetic interactions between Buchnera and aphids. The 16S rRNA gene was amplified and sequenced for this purpose and a phylogenetic tree built up by the neighbor-joining method. A significant correlation between B. aphidicola genes and the aphid species was revealed by this phylogenetic tree and the haplotype network. Specimens collected in Feke from Solanum melongena L. was distinguished from the other B. aphidicola genes on Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) with a high bootstrap value of 99. Buchnera aphidicola in Myzus spp. was differentiated from others, and the difference between Myzus cerasi (Fabricius, 1775) and Myzus persicae (Sulzer, 1776) was clear. Although, B. aphidicola is specific to its host aphid, certain nucleotide differences obtained within the species could enable specification to geographic region or host plant in the future.

Keywords

• Aphid,genetic similarity,phylogenetics,symbiotic bacterium

Kaynakça

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