Türkiye' den Andricus quercustozae (Bosc, 1792) (Hymenoptera: Cynipidae) mazı arılarının PCR-RFLP varyasyonu

Öz

Bu çalışmada ülkemizde bulunan Andricus quercustozae (Bosc, 1792)’nin tür içi mitokondriyal DNA çeşitliliği 2006-2008 yılları arasında yapılan arazi çalışmalarında toplanan örneklerle PCR-RFLP yöntemi kullanılarak çalışılmıştır. Onaltı populasyondan toplanan 95 bireyde toplam 28 haplotip belirlenmiştir. Haplotip ve nükleotid çeşitliliği sırasıyla 0.45 ve 0.05 olarak hesaplanmıştır. Analiz edilen populasyonlar arasında en fazla nükleotid farklılaşması % 9.0 olarak hesaplanmıştır bu da A. quercustozae haplotipleri arasında oldukça eski bir ayrılmayı ifade etmektedir. PHYLIP programı kullanılarak elde edilen dendrogram haplotiplerin coğrafik dağılımları ile oluşturdukları gruplar arasında bir bağıntının olduğunu göstermektedir. Genetik farklılaşmanın dağılımını belirlemek için yapılan AMOVA analizi istatistiksel olarak anlamlı çıkmıştır. Bu çalışma A. quercustozae populasyonlarının oldukça fazla genetik çeşitliliğe sahip olduğunu ve coğrafik olarak belirli gruplaşmalar yaptığını ortaya koymaktadır. Elde edilen veriler ışığında çalışılan türün populasyon genetik yapısının şekillenmesinde ülkemiz topografyasının ve evrimsel tarihinin etkili olduğu sonucu çıkarılabilir. 

Anahtar Kelimeler

• Andricus quercustozae
• Cynipidae
• meşe mazı arısı
• PCR-RFLP

PCR-RFLP variation of the oak gall wasp, Andricus quercustozae (Bosc, 1792) (Hymenoptera: Cynipidae) from Turkey

Abstract

Oak gall wasp specimens collected in the summer of 2006-2008 from Turkey were studied using PCR-RFLP method for revealing intraspecific mitochondrial DNA variation of Andricus quercustozae (Bosc, 1792). A total of 28 haplotypes were detected among 95 individuals collected from 16 populations. The estimated average haplotype and nucleotide diversity were 0.45 and 0.05, respectively. The highest nucleotide divergence estimate among the analyzed oak gall wasp populations was 9.0% indicating an ancient split between A. quercustozae haplotypes. Dendrogram obtained using PHYLIP program indicated that there was a significant relationship between geographical distribution of haplotypes and their clustering. AMOVA analysis for estimation of the partitioning of genetic differentiation was statistically significant. This study shows that A. quercustozae populations have high amount of genetic diversity and form geographically significant groupings. It seems that varied topography and evolutionary history of Turkey play an important role in shaping the current population genetic structure of the species

Keywords

• Andricus quercustozae
• Cynipidae
• oak gall wasp
• PCR-RFLP

Kaynakça

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