Effects of diazinon on antioxidant enzymes and adult emergence of parasitoid Pimpla turionellae L. (Hymenoptera:Ichneumonidae)

Abstract

Keywords

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Diazinonun parasitoid Pimpla turionellae 'nin (Hymenoptera:Ichneumonidae) antioksidan enzimleri ve ergin birey çıkışına etkileri

Öz

Organofosforlu bir insektisitin Pimpla turionellae’nin antioksidan enzim aktivitelerine ve toplam ve dişi birey çıkışına olan etkileri 0.01, 0.10, 0.25, 0.50 ve 0.75 ppm diazinon içeren sentetik besin kullanılarak araştırıldı. Subletal diazinon konsantrasyonları, antioksidan enzim aktivitelerini belirlemek için 24, 48, 72 ve 96 saat süreyle, ergin birey çıkışını belirlemek için ise 31 gün boyunca P. turionellae’ya verildi. P. turionellae insektisit stresi altında diazinonun dozuna ve maruz kalma süresine bağlı olarak farklı tepkiler gösterdi. Diazinon özellikle yüksek konsantrasyonlarda (0.50 ve 0.75 ppm) SOD aktivitesinin kontrole göre önemli ölçüde artmasına neden oldu. Bununla beraber CAT aktivitesinde düzensiz artışlara neden oldu. Çalışmadaki önemli bir bulgu, CAT aktivitesi hiçbir konsantrasyonda kontrol seviyesinin altına düşmedi. Toplam ergin ve dişi çıkışı diazinondan önemli ölçüde etkilenerek azaldı. Sonuç olarak, SOD’nin P. turionellae’de diazinon toksisitesine karşı çok önemli bir koruyucu rol oynadığı ve diazinonun toplam ve dişi birey çıkışını önemli ölçüde azalttığı belirlendi

Anahtar Kelimeler

• Pimpla turionellae, antioksidan enzimler, insektisit stresi, verimlilik, diazinon

Kaynakça

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