Metil öjenol ve diyatom toprağının depolanmış ürün zararlısı böceklere karşı tek başına ve birlikte etkisi

Öz

Tescilli bir diyatom toprağı Detech ve metil öjenolün Sitophilus granarius (L., 1758), Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae), Rhyzopertha dominica (Fabricius, 1792) (Coleoptera: Bostrychidae), Tribolium confusum Jaquelin Du Val, 1868 (Coleoptera: Tenebrionidae) erginlerine karşı Zirai Mücadele Merkez Araştırma Enstitüsü, Depolanmış Ürün Zararlıları Laboratuvarı (Ankara, Türkiye)’nda 2020 yılında test edilmiştir. Bu amaçla farklı dozlarda Detech (100, 250 ve 500 mg kg-1) ve metil öjenol (5, 10 ve 15 mg kg-1) kullanılmıştır. Böceklerin ölüm oranını belirlemek için farklı maruz kalma süreleri kullanılmıştır. Sinerjistik etkiyi belirlemek amacıyla, her iki ürünün de yarı dozları böcekler üzerinde çalışılmıştır. Diyatom toprağı Detech, R. dominica, S. zeamais, S. granarius ve T. confusum için en yüksek etkisi 500 mg kg-1 dozda, 28 günlük maruziyet süresinin sonunda sırasıyla %81, %29, %26 ve %18 ölüm oranı şeklinde gerçekleşmiştir. 15 mg kg-1’lık metil öjenol dozu, R. dominica için günlük maruziyetten sonra %100 ölüm oranı sağlamıştır. Her iki ürünün aynı anda kullanılması, üzerinde çalışılan böceklerde tam bir ölüm oranına ulaşmamıştır. Elde edilen veriler, Detech ve metil öjenol kullanımının depolanmış ürün zararlısı böcekler ile mücadelede ümit var sonuçlar verdiğini ortaya koymuştur.

Anahtar Kelimeler

• Uçucu yağlar
• ölüm oranı
• fiziksel kontrol
• depolanmış ürün zararlısı böcekler
• sinerjizm

Combined and individual effects of diatomaceous earth and methyl eugenol against stored products insect pests

Abstract

The insecticidal activity of a proprietary diatomaceous earth, Detech, and methyl eugenol was tested with Sitophilus granarius (L., 1758), Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae), Rhyzopertha dominica (Fabricius, 1792) (Coleoptera: Bostrychidae), Tribolium confusum Jaquelin Du Val, 1868 (Coleoptera: Tenebrionidae) adults at Stored Products Pest Laboratory of Plant Protection Central Research Institute (Ankara, Turkey) in 2020. A range of doses of Detech (100, 250 and 500 mg kg-1) and methyl eugenol (5, 10 and 15 mg kg-1) were used. To determine insect mortality, at a range of times after treatment, and half-doses of both products were also studied in order to examine simultaneous use for synergistic effect. The greatest effect of Detech on R. dominica, S. zeamais, S. granarius and T. confusum was observed with 500 mg kg-1 giving 81, 29, 26 and 18% mortality 28 d after treatment (DAT), respectively. Methyl eugenol at 15 mg kg-1 gave complete mortality with R. dominica adults 3 DAT. Simultaneous application of both products did not give complete mortality in test insects. The data show that Detech and methyl eugenol are promising treatments for the control of insect pests of stored products.

Keywords

• Essential oils
• mortality
• physical control
• stored products insects
• synergism

Kaynakça

1. Adarkwah, C., D. Obeng-Ofori, C. Ulrichs & M. Schöller, 2017. Insecticidal efficacy of botanical food by-products against selected stored-grain beetles by the combined action with modified diatomaceous earth. Journal of Plant Diseases and Protection, 124 (3): 255-267.
2. Aitken A. D., 1975. Insect Travelers. Volume I. Coleoptera. Technical Bulletin, Ministry of Agriculture, Fisheries and Food, No. 31, 191 pp.
3. Alkan, M., 2020a. Chemical composition and insecticidal potential of different Origanum spp. (Lamiaceae) essential oils against four stored product pests. Turkish Journal of Entomology, 44 (2): 149-163.
4. Alkan, M., 2020b. Chemical composition of Achillea millefolium L. (Asteraceae) essential oil and insecticidal effect against Sitophilus granarius (Coleoptera: Curculionidae) and Rhyzopertha dominica (Coleoptera: Bostrichidae). Plant Protection Bulletin, 60 (1): 85-93.
5. Alkan, M., T. Atay, S. Ertürk & İ. Kepenekci, 2019a. Comparison of bioactivities of native diatomaceous earth against Turkestan cockroach [Blatta lateralis Walker (Blattodea: Blattidae)] nymphs. Applied Ecology and Environmental Research, 17 (3): 5987-5994.
6. Alkan, M., S. Erturk, T. A. Firat & E. Ciftci, 2019b. Study of insecticidal and behavioral effects and some characteristic of native diatomaceous earth against the bean weevil, Acanthoscelides obtectus (Coleoptera: Chrysomelidae). Fresenius Environmental Bulletin, 28 (4): 2916-2922.
7. Alkan, M., S. Ertürk, T. Atay & A. Çağlayan, 2019c. Insecticidal efficacy of local diatomaceous earths against adult and larvae of Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae). Turkish Journal of Entomology, 43 (3): 347-354.
8. Arthur, F. H., 1996. Grain protectants: current status and prospects for the future. Journal of Stored Products Research, 32 (4): 293-302.

9. Ashraf, M., W. Wakil, F. Hafeez & M. Farooq, 2016. Persistence and insecticidal efficacy of a diatomaceous earth formulation, inert-PMS, in stored wheat grain against Cryptolestes ferrugineus (Stephens), Liposcelis paeta Pearman, Rhyzopertha dominica (F.) and Tribolium castaneum (Herbst). Turkish Journal of Entomology, 40 (2): 107-115.
10. Athanassiou, C. G. & N. Kavallieratos, 2005. Insecticidal effect and adherence of PyriSec® in different grain commodities. Crop Protection, 24 (8): 703-710.
11. Athanassiou, C. G., N. G. Kavallieratos & N. S. Andris, 2004. Insecticidal effect of three diatomaceous earth formulations against adults of Sitophilus oryzae (Coleoptera: Curculionidae) and Tribolium confusum (Coleoptera: Tenebrionidae) on oat, rye and triticale. Journal of Economic Entomology, 97 (6): 2160-2167.
12. Athanassiou, C. G., N. G. Kavallieratos, A. Chiriloaie, T. N. Vassilakos, V. Fatu, S. Drosu & R. Dudoiu, 2016. Insecticidal efficacy of natural diatomaceous earth deposits from Greece and Romania against four stored grain beetles: the effect of temperature and relative humidity. Bulletin of Insectology, 69 (1): 25-34.
13. Baldassari, N., C. Prioli, A. Martini, V. Trotta & P. Baronio, 2008. Insecticidal efficacy of a diatomaceous earth formulation against a mixed age population of adults of Rhyzopertha dominica and Tribolium castaneum as function of different temperature and exposure time. Bulletin of Insectology, 61 (2): 355-360.
14. Baliota, G. V. & C. G. Athanassiou, 2020. Evaluation of a Greek diatomaceous earth for stored product insect control and techniques that maximize its insecticidal efficacy. Applied Sciences, 10 (18): 6441.
15. Barres, B., A. Micoud, M. F. Corio-Costet, D. Debieu, S. Fillinger, A. S. Walker, C. Délye, J. Grosman & M. Siegwart, 2016. Trends and challenges in pesticide resistance detection. Trends in Plant Science, 21 (10): 834-853.
16. Bayram, A., A. A. Isikber, Ö. Sağlam & R. Şen, 2020. “Evaluation of repellency effect of diatomaceous earth formulation (Detech®) on three coleopteran stored grain insects, 56-64”. 12th Conference of the Working Group Integrated Protection of Stored Product (September 3-6, 2019, Pisa, Italy), 429 pp.
17. Boulogne, I., P. Petit, H. Ozier-Lafontaine, L. Desfontaines & G. Loranger-Merciris, 2012. Insecticidal and antifungal chemicals produced by plants: a review. Environmental Chemistry Letters, 10 (4): 325-347.
18. Boyer, S., H. Zhang & G. Lempérière, 2012. A review of control methods and resistance mechanisms in stored-product insects. Bull Entomol Res 102 (2): 213-229.
19. Carlson, S. D. & H. J. Ball, 1962. Mode of action and insecticidal value of a diatomaceous earth as a grain protectant. Journal of Economic Entomology, 55 (6): 964-970.
20. Chang, C. L., I. K. Cho & Q. X. Li, 2009. Insecticidal activity of basil oil, trans-anethole, estragole, and linalool to adult fruit flies of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae. Journal of Economic Entomology, 102 (1): 203-209.
21. Dawson, W. A. J. M., M. Jestoi, A. Rizzo, P. Nicholson & G. L. Bateman, 2004. Field evaluation of fungal competitors of Fusarium culmorum and F. graminearum, causal agents of ear blight of winter wheat, for the control of mycotoxin production in grain. Biocontrol Science and Technology, 14 (8): 783-799.
22. Doumbia, M., B. G. Douan, K. E. Kwadjo, D. K. Kra, V. Martel & M. Dagnogo, 2014. Effectiveness of diatomaceous earth for control of Sitophilus zeamais (Coleoptera: Curculionidae), Tribolium castaneum and Palorus subdepressus (Coleoptera: Tenebrionidae). Journal of Stored Products Research, 57: 1-5.
23. Dwivedy, A. K., M. Kumar, N. Upadhyay & N. K. Dubey, 2015. Green chemistry in agricultural pest management programmes. Medicinal Chemistry, (Special Issue): 1-2.
24. Ebeling, W., 1971. Sorptive dusts for pest control. Annual Review of Entomology, 16 (1): 123-158.
25. Ertürk, S., T. Atay, U. Toprak & M. Alkan, 2020. The efficacy of different surface applications of wettable powder formulation of Detech® diatomaceous earth against the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Journal of Stored Products Research, 89: 101725.
26. Ertürk, S., A. G. Ferizli & M. Emekci, 2017. Evaluation of diatomaceous earth formulations for the control of rice weevil, Sitophilus oryzae (L.), 1763 (Coleoptera: Curculionidae) in stored rice. Turkish Journal of Entomology, 41 (3): 347-354.
27. Ertürk, S., A. Yılmaz, T. Fırat Akdeniz & M. Alkan, 2014. Fumigant effect of trans-anethole and carbon dioxide mixture against to Rhyzopertha dominica, Tribolium castaneum and Sitophilus oryzae. Plant Protection Bulletin, 57 (3): 391-400.
28. FAO, 2009. How to feed the world in 2050. (Web page: http://www.fao.org/fileadmin/templates/wsfs/docs/expert_paper/ How_to_Feed_the_World_in_2050.pdf) (Date accessed: October 2020).
29. Ferizli, A. G., G. Beriş & M. Emekçi, 2012. Effects of diatomaceous earth on the mortality and progeny production of Rhyzopertha dominica (Coleoptera: Bostrychidae). Journal of Agricultural Sciences, 17 (2): 85-94.
30. Gałęcki, R., T. Bakuła, M. Wojtacki & K. Żuk-Gołaszewska, 2019. Susceptibility of ancient wheat species to storage pests Sitophilus granarius and Tribolium confusum. Journal of Stored Products Research, 83: 117-122.
31. Guo, S. S., W. J. Zhang, C. X. You, J. Y. Liang, K. Yang, Z. F. Geng & C. F. Wang, 2017. Chemical composition of essential oil extracted from Laggera pterodonta and its bioactivities against two stored product insects. Journal of Food Processing and Preservatio, 41 (2): 1-9.
32. Halliday, W. R., F. H. Arthur & J. L. Zettler, 1988. Resistance status of red flour beetle (Coleoptera: Tenebrionidae) infesting stored peanuts in the southeastern United States. Journal of Economic Entomology, 81 (1): 74-77.
33. Herrmann, K. M. & L. M. Weaver, 1999. The shikimate pathway. Annual Review of Plant Physiology and Plant Molecular Biology, 50 (1): 473-503.
34. Houssou, P. A., B. C. Ahohuendo, P. Fandohan, K. Kpodo, D. J. Hounhouigan & M. Jakobsen, 2009. Natural infection of cowpea (Vigna unguiculata (L.) Walp.) by toxigenic fungi and mycotoxin contamination in Benin, West Africa. Journal of Stored Products Research, 45 (1): 40-44.
35. Hubert, J., V. Stejskal, C. G. Athanassiou & J. E. Throne, 2018. Health hazards associated with arthropod infestation of stored products. Annual Review of Entomology, 63 (1): 553-573.
36. Imai, T. & R. Masuda, 2017. Insecticidal activities of methyleugenol and β-asarone, from the herbal medicines Saishin and Sekishōkon, and other alkoxy-propenyl-benzene derivatives against the cigarette beetle Lasioderma serricorne (Coleoptera: Anobiidae). Applied Entomology and Zoology, 52 (2): 183-188.
37. Islam, M. S. & M. M. Rahman, 2016. Diatomaceous earth induced alterations in the reproductive attributes in the housefly Musca domestica L. (Diptera: Culicidae). Elixir Applied Zoology, 96 (2016): 41241-41244.
38. Jankowska, M., J. Rogalska, J. Wyszkowska & M. Stankiewicz, 2017. Molecular targets for components of essential oils in the insect nervous system-a review. Molecules, 23 (1): 34 (1-20).
39. Kavallieratos, N. G., C. G. Athanassiou, F. G. Pashalidou, N. S. Andris & Zˇ. Tomanovic´, 2005. Influence of grain type on the insecticidal efficacy of two diatomaceous earth formulations against Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae). Pest Management Science, 61 (7): 660-666.
40. Khorrami, F., O Valizadegan, M. Forouzan & A. Soleymanzade, 2018. The antagonistic/synergistic effects of some medicinal plant essential oils, extracts and powders combined with diatomaceous earth on red flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). Archives of Phytopathology and Plant Protection, 51 (13-14): 685-695.
41. Korunić, Z., 1998. Diatomaceous earths, a group of natural insecticides. Journal of Stored Products Research, 34 (2-3): 87-97.
42. Korunić, Z. & P. G. Field, 2020. Evaluation of three new insecticide formulations based on inert dusts and botanicals against four stored-grain beetles. Journal of Stored Products Research, 88: 101633.
43. Korunić, Z., A. Liška, P. Lucić, D. Hamel & V. Rozman, 2020. Evaluation of diatomaceous earth formulations enhanced with natural products against stored product insects. Journal of Stored Products Research, 86: 101565.
44. Krzyzowski, M., J. Francikowski, B. Baran & A. Babczynska, 2019. Physiological and behavioral effects of different concentrations of diatomaceous earth on common stored product pest Callosobruchus maculatus. Journal of Stored Products Research, 82: 110-115.
45. Lee, S. E., B. H. Lee, W. S. Choi, B. S. Park, J. G. Kim & B. C. Campbell, 2001. Fumigant toxicity of volatile natural products from Korean spices and medicinal plants towards the rice weevil, Sitophilus oryzae (L). Pest Management Science, 57 (6): 548-553.
46. Liang, J. Y., W. T. Wang, Y. F. Zheng, D. Zhang, J. L. Wang, S. S. Guo & J. Zhang, 2017. Bioactivities and chemical constituents of essential oil extracted from Artemisia anethoides against two stored product insects. Journal of Oleo Science, 66 (1): 71-76.
47. Losic, D. & Z. Korunić, 2017. “Diatomaceous Earth, a Natural Insecticide for Stored Grain Protection: Recent Progress and Perspectives, 219-247”. In: Diatom Nanotechnology: Progress and Emerging Applications (Ed. D. Losic). Royal Society of Chemistry Publishing, Cambridge, UK, 270 pp.
48. Mckenzie, Jr. J. D. & J. M. R. Goldman, 2004. The Student Guide to MINITAB Release 14 + MINITAB Student Release 14 Statistical Software (Book + CD). Pearson Education, Boston, MA. 560 pp.
49. Merget, R., T. Bauer, H. Küpper, S. Philippou, H. Bauer, R. Breitstadt & T. Bruening, 2002. Health hazards due to the inhalation of amorphous silica. Archives of Toxicology, 75 (11-12): 625-634.
50. Mewis, I. & C. Ulrichs, 2001. Action of amorphous diatomaceous earth against different stages of the stored product pests Tribolium confusum, Tenebrio molitor, Sitophilus granarius and Plodia interpunctella. Journal of Stored Products Research, 37 (2): 153-164.
51. Mossa, A. T. H., 2016. Green pesticides: essential oils as biopesticides in insect-pest management. Journal of Environment Science and Technology, 9 (5): 354-378.
52. Norambuena, C., G. Silva, A. Urbina, I. Figueroa & J. C. Rodríguez-Maciel, 2016. Insecticidal activity of Laureliopsis philippiana (Looser) Schodde (Atherospermataceae) essential oil against Sitophilus spp. (Coleoptera: Curculionidae). Chilean Journal of Agricultural Research, 76 (3): 330-336.
53. Obeng-Ofori, D. & C. Reichmuth, 1997. Bioactivity of eugenol, a major component of essential oil of Ocimum suave (Wild.) against four species of stored-product Coleoptera. International Journal of Pest Management, 43 (1): 89-94.
54. Oliveira, C. M., A. M. Auad, S. M. Mendes & M. R. Frizzas, 2014. Crop losses and the economic impact of insect pests on Brazilian agriculture. Crop Protection, 56: 50-54.
55. Pacheco, I. A., M. R. Sartori & S. Bolonhezi, 1990. “Resistance to malathion, pirimiphos-methyl and fenitrothion in Coleoptera from stored grains, 1029-1038”. Proceedings of the 5th International Working Conference on Stored Product Protection (9-14 September 1990, Bordeaux, France), 2064 pp.
56. Paponja, I., V. Rozman & A. Liška, 2020. Natural formulation based on diatomaceous earth and botanicals against stored product insects. Insects, 11 (9): 613.
57. Prasantha, B. D. R., C. Reichmuth, C. Adler & D. Felgentreu, 2015. Lipid adsorption of diatomaceous earths and increased water permeability in the epicuticle layer of the cowpea weevil Callosobruchus maculatus (F.) and the bean weevil Acanthoscelides obtectus (Say) (Chrysomelidae). Journal of Stored Products Research, 64 (Part A): 36-41.
58. Regnault-Roger, C., C. Vincent & J. T. Arnason, 2012. Essential oils in insect control: low-risk products in a high-stakes world. Annual Review of Entomology, 57: 405-424.
59. Ribeiro, B. M., R. N. C. Guedes, E. E. Oliveira & J. P. Santos, 2003. Insecticide resistance and synergism in Brazilian populations of Sitophilus zeamais (Coleoptera: Curculionidae). Journal of Stored Products Research, 39 (1): 21-31.
60. Richard, E., N. Heutte, L. Sage, D. Pottier, V. Bouchart, P. Lebailly & D. Garon, 2007. Toxigenic fungi and mycotoxins in mature corn silage. Food and Chemical Toxicology, 45 (12): 2420-2425.
61. Rigaux, M., E. Haubruge & P. G. Fields, 2001. Mechanisms for tolerance to diatomaceous earth between strains of Tribolium castaneum (Coleoptera: Tenebrionidae). Entomologia Experimentalis et Applicata, 101 (1): 33-39.
62. Sağlam, Ö., R. Şen, H. Bozkurt & A. A. Işıkber, 2020. “Insecticidal efficacy of three commercial diatomaceous earths, Detech®, Demite® and Silicosec® against cowpea weevil, Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchinae), 47-53”. 12th Conference of the Working Group Integrated Protection of Stored Product (September 2019, Pisa, Italy), 429 pp.
63. Shojaei, A., K. Talebi, I. Sharifian & S. M. Ahsaei, 2017. Evaluation of detoxifying enzymes of Tribolium castaneum and Tribolium confusum (Col.: Tenebrionidae) exposed to essential oil of Artemisia dracunculus L. Biharean Biologist, 11 (1): 5-9.
64. Smith, R. L., T. B. Adams, J. Doull, V. J. Feron, J. I. Goodman, L. J. Marnett & I. G. Sipes, 2002. Safety assessment of allylalkoxybenzene derivatives used as flavouring substances-methyl eugenol and estragole. Food and chemical Toxicology, 40 (7): 851-870.
65. Tan, K. H. & R. Nishida, 2012. Methyl eugenol: its occurrence, distribution, and role in nature, especially in relation to insect behavior and pollination. Journal of Insect Science, 12 (1): 1-74.
66. Upadhyay, N., A. K. Dwivedy, M. Kumar, B Prakash & N. K. Dubey, 2018. Essential oils as eco-friendly alternatives to synthetic pesticides for the control of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Journal of Essential Oil Bearing Plants, 21 (2): 282-297.
67. Vayias, B. J. & C. G. Athanassiou, 2004. Factors affecting the insecticidal efficacy of the diatomaceous earth formulation SilicoSec against adults and larvae of the confused flour beetle, Tribolium confusum DuVal (Coleoptera: Tenebrionidae). Crop Protection, 23 (7): 565-573.
68. Warton, D. I. & F. K. C. Hui, 2011. The arcsine is asinine: the analysis of proportions in ecology. Ecology, 92 (1): 3-10.
69. Zacker, F. & G. Kunike, 1931. Untersuchungen uber die insektizide Wirkung von Oxyden und Karbonaten. Arbeiten aus der Biologischen Reichsanstalt für Land- und Forstwirtschaft, 18 (2): 201-231.
70. Zar, J. H., 1999. Biostatistical Analysis, Fourth Edition. Published by PrenticeHall, Upper Saddle River, New Jersey, USA, 929 pp.
71. Zhanda, J., B. M. Mvumi & H. Machekano, 2020. Potential of three enhanced diatomaceous earths against Sitophilus zeamais Motschulsky and Prostephanus truncantus (Horn) on stored maize grain. Journal of Stored Products Research, 87: 101608.