Detection and identification of citrus long-horned beetle, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae) a new pest in Antalya Province, Turkey by sequencing of mtCOI region

Yıl 2017, Cilt: 41 Sayı: 3, 325 - 331, 08.11.2017

Nurdan Topakcı Utku Yükselbaba Hüseyin Gocmen

https://doi.org/10.16970/entoted.320617

Cited By: 4

Öz

In
2016, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae)
was recorded for the first time on Acer negundo L. in Antalya
Province, Turkey. Genetic identification of adult samples was made based on
approximate alignment of a 729-bp sequence of the mitochondrial cytochrome
oxidase I gene region. The sequence alignment in the BLAST analysis from the
NCBI Genbank showed 99% similarity with A. chinensis. The sequence data
was deposited to NCBI Genbank. A phylogenetic analysis was performed by using
the sequence data belonging to A. chinensis and some species of same
genus obtained from the NCBI. According to the phylogenetic analysis,
populations of A. chinensis from Antalya and China were placed in the
same subgroup and therefore it is postulated that the origin of the Antalya
population is China.   

Anahtar Kelimeler

Anoplophora chinensis, DNA barcoding, mtCOI, phylogenetic analysis

Antalya ilinde yeni bir zararlı Turunçgil uzun antenli böceği, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae)’in tespiti ve mtCOI gen bölgesi sekansına göre tanımlanması

Öz

Bu çalışma ile Anoplophora
chinensis (Forster, 1771) (Coleoptera: Cerambycidae) Antalya ilinde ilk kez
2016 yılında Acer negundo L. bitkisi üzerinde tespit edilmiştir.
Bununla birlikte, çalışmada A. chinensis’e ait ergin bireylerden DNA
ekstraksiyonu yapılmıştır. Elde edilen DNA örneklerinin mitokondrial sitokrom
oksidaz I gen bölgesinin amplifikasyonu sonucunda yaklaşık 729 bç sekans
dizilimi elde edilmiştir. Elde edilen veriler, NCBI Genbank’ da BLAST analizine
tabi tutulmuş ve A. chinensis ile %99 benzerlik gösterdiği tespit
edilmiştir. Sekans dizilimi NCBI’a kaydedilmiş, Genbank’ta A. chinensis
ve aynı cinse ait bazı türlerin sekans verilerinden yararlanılarak filogenetik
analiz yapılmıştır. Filogenetik analize göre Antalya A. chinensis
popülasyonu Çin popülasyonu ile aynı alt grupta yer almış ve Antalya
popülasyonunun kökeninin Çin olabileceği ağırlık kazanmıştır.

Anahtar Kelimeler

Anoplophora chinensis, DNA barkodlama, mtCOI, filogenetik analiz

Kaynakça

• Anonymous, 2015. http://www.eppo.int/QUARANTINE/special_topics/anoplophora_chinensis /chinensis_IT_2007.htm (accessed: 19.09.2015).
• EPPO Standard PM 9/16 (1), 2013. Anoplophora chinensis: procedures for official control. OEPP/EPPO Bulletin, 43:518–526.
• Felsenstein, J., 1993. PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle.
• Folmer, 0., M. Black, W. Hoeh, R. Lutz & R. Vrijenhoek, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3(5), 294-299.
• Ge, X., S. Zong, S. He, Y. Liu & X. Kong, 2014. Areas of China predicted to have a suitable climate for Anoplophora chinensis under a climate-warming scenario. Entomologia Experimentalis et Applicata. 153(3):256–265.
• Gyeltshen, J. & A. Hodges, 2005. Citrus Longhorned Beetle, EENY-357. University of Florida: http://edis.ifas.ufl.edu/IN633
• Haack, RA., F. Hérard, J. Sun & J.J. Turgeon, 2010. Managing invasive populations of Asian longhorned beetle and citrus longhorned beetle: a worldwide perspective. Annu Rev Entomol. 55:521-46.
• Herard, F., M. Ciampitti, M. Maspero, H. Krehan, U. Benker & C. Boegel, 2006. Anoplophora species in Europe: infestations and management processes. EPPO Bulletin, 36:470–474.CrossRef.
• Hızal, E., Z. Arslangündoğdu, A. Göç & M. Ak, 2015. The new record for Turkish invasive alien insect fauna Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae). Journal of the Faculty of Forestry Istanbul University. 65(1): 7-10.
• Hu, J., S. Angeli, S. Schuetz, Y. Luo & A. E. Hajek, 2009. Ecology and management of exotic and endemic Asian longhorned beetle Anoplophora glabripennis. Agricultural and Forest Entomology, 11:359–375.
• Japan Plant Protection Society, ed. 1997. Syokubutu boueki kouza (=Textbook of Plant Protection). Pest Insects and Animals, 3rd edition. Tokyo, Japan: Japan Plant Protection Society.
• Lawrence, J. F., 1982. Coleoptera, In: Parker, S. (ed.). Synopsis and Classification of Living Organisms. McGraw Hill, New York, 482-553.
• Li, W., X. Yang, L. Qian, Y. An & J. Fang, 2015. The complete mitochondrial genome of the citrus long-horned beetle, Anoplophora chinensis (Coleoptera: Cerambycidae). Mitochondrial DNA, Early Online: 1–3. ISSN: 1940-1736 (print), 1940-1744 (electronic)
• Lieu, K.O.V., 1945. The study of wood borers in China I: Biology and control of the citrus-root-cerambycids, Melanauster chinensis, Forster (Coleoptera). The Florida Entomologist 27:61-101.
• Lingafelter, S.W. & E.R. Hoebeke, 2002. Revision of Anoplophora (Coleoptera: Cerambycidae). Entomological Society of Washington, D.C. 236.
• Maspero, M., 2015. Managıng Invasive Populatıons of Anoplophora chinensis and A. glabripennis in Lombardy Region. Dottorato Di Ricerca in Scienze e Tecnologie Agrarie, Ambiientali e Alimentari. Alma Mater Studiorum – Università di Bologna.
• Muraji, M., S. Wakamura, H. Yasui, N. Arakaki, Y. Sadoyama, S. Ohno, & K. Matsuhira, 2011. Genetic variation of the white-spotted longicorn beetle Anoplophora spp. (Coleoptera: Cerambycidae) in Japan detected by mitochondrial DNA sequence. Applied Entomology and Zoology, 46(3):363–373.
• Ohbayashi, N., J. Ogawa & Z.H. Su, 2009. Phylogenetic Analysis of the Lamiine Genus Anoplophora and its Relatives (Coleoptera, Cerambycidae) Based on the Mitochondrial COI Gene. Spec. Bull. Jpn. Soc. Coleopterol., 7: 309-324.
• Peverieri, G. S. & P. F. Roversi, 2010. Feeding and oviposition of Anoplophora chinensis on ornamental and forest trees. Phytoparasitica, 38(5): 421–428.
• Saitou, N. & M. Nei, 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4:406– 425.
• Simon, C., F. Frati, A. Beckenbach, B. Crespi, H. Liu, & P. Flook, 1994. Evoluation, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann. Entomol. Soc. Am., 87:651-701.
• Sjöman, H., J. Ostberg, & J. Nilsson, 2014. Review of Host Trees for the Wood-Boring Pests Anoplophora glabripennis and Anoplophora chinensis: An Urban Forest Perspective Arboriculture & Urban Forestry, 40(3): 143–164.
• Strangi, A., G. Sabbatini Peverieri, & P. F. Roversi, 2013. Managing outbreaks of the citrus long-horned beetle Anoplophora chinensis (Forster) in Europe: molecular diagnosis of plant infestation. Pest Manag. Sci. 69(5): 627–63.
• Yang, P., 1984. The application of Oecophylla smaragdina Fabr. in South Fujian. Fujian Agricultural Science and Technology, 5:23-25.
• Yükselbaba, U, H. Göçmen, 2016. Determination of genetic variation of tomato leafminer [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)] populations from west mediterreanean region of Turkey based on mitochondrial cytochrome oxidase I (mtCOI). Mediterranean Agricultural Sciences, 29(1):5-7.