Effect of entomopathogenic nematode species on the corn stalk borer (Sesamia cretica Led. Lepidoptera: Noctuidae) at different temperatures

Abstract

Three Turkish strains of the entomopathogenic nematodes, Steinernema carpocapsae (Adapazarı), S. feltiae (Çanakkale) and Heterorhabditis bacteriophora (Kırklareli), were tested in the laboratory for their virulence on the last instar larvae of the corn stalk borer, Sesamia cretica. Mortality rates of last instar S. cretica larvae were determined for each nematode species under four different temperature treatments. Accordingly, at 15 ºC, mortalities of S. cretica exposed to S. carpocapsae, S. feltiae and H. bacteriophora were 48, 56 and 14%, respectively. These mortalities at 20 ºC were 62, 76 and 50%; at 25 ºC, 82, 90 and 90%; and at 30 ºC (the highest application temperature), mortality was 82, 92 and 94%, respectively. These results show that S. carpocapsae and S. feltiae caused similar mortalities at all applied temperatures and their effectiveness increased as the temperature increased. Although H. bacteriophora caused low mortality at low temperatures, it infected and caused very high levels of mortality at relatively high temperatures.

Keywords

• Sesamia cretica, Steinernema carpocapsae, Steinernema feltiae, Heterorhabditis bacteriophora

Entomopatojen nematod türlerinin farklı sıcaklıklarda mısır koçan kurdu (Sesamia cretica Led. Lepidoptera: Noctuidae) üzerindeki etkinliği

Öz

Entomopatojen nematodlar; Steinernema carpocapsae (Adapazarı), S. feltiae (Çanakkale) ve Heterorhabditis bacteriophora’nın (Kırklareli) üç Türk izolatının mısır koçan kurdu Sesamia cretica’nın son dönem larvalarına karşı etkinlikleri araştırılmıştır. Entomopatojen nematodlar tarafından infekte edilen son dönem Sesamia cretica larvalarındaki ölüm oranları nematod türüne ve uygulandığı sıcaklığa bağlı olarak değişmekle birlikte 15 o C’de, S. carpocapsae, S. feltiae ve H. bacteriophora için sırası ile % 48, 56 ve 14 olarak tespit edilmiştir. Bu oran 20 o C’de, % 62, 76 ve 50, 25 o C’de % 82, 90 ve 90, denemede kullanılan en yüksek sıcaklık olan 30 o C’de ise % 82, 92 ve 94 olarak bulunmuştur. Elde edilen bu sonuçlar S. carpocapsae ve S. feltiae’nin uygulandığı tüm sıcaklıklarda birbirine yakın sonuçlar verdiğini ve sıcaklığın artması ile etkinliklerinin de arttığını, H. bacteriophora’nın ise düşük sıcaklıklarda oldukça düşük olan infekte etme oranının, yüksek sıcaklıklarda çok yüksek seviyelere ulaştığını ve en iyi infeksiyonun bu sıcaklık derecelerinde olduğunu ortaya koymuştur.

Anahtar Kelimeler

• Sesamia cretica, Steinernema carpocapsae, Steinernema feltiae, Heterorhabditis bacteriophora.

Kaynakça

1. Burnell, A.M. & S.P. Stock, 2000. Heterorhabditis, Steinernema and their bacterial symbionts-lethal pathogens of insects. Nematology, 2: 31-42.
2. Dutky, S.R., J.V. Thompson & G.E. Cantwell, 1964. A technique for the mass propagation of the DD-136 nematode. Journal of Insect Pathology, 5: 417-422.
3. El-Wakeil, M. A.Hussein, 2009. Field performance of entomopathogenic nematodes and an egg parasitoid for suppression of corn borers in Egypt. Archives of Phytopathology and Plant Protection, 42(3): 228–237.
4. Fenton, A., R. Norman, JP. Fairbairn & P.J. Hudson, 2000. Modelling the efficacy of entomopathogenic nematodes in the regulation of invertebrate pests in glasshouse. Journal of Applied Ecology, 37: 309-320.
5. Gaugler, R. & H.K. Kaya, 1990. Entomopathogenic Nematodes in Biological Control. CRC Press, Boca Raton, FL, 365 pp.
6. Georgis, R. & S.A. Manweiler, 1994. Entomopathogenic nematodes: A developing biological control technology. Agricultural Zoology Reviews, 6: 63-94.
7. González-Ramírez, M., R. Lezama-Gutiérrez, J. Molina-Ochoa, O. Rebolledo-Domínguez, M. López-Edwards & A. Pescador-Rubio, 2000. Susceptibility of Mocis latipes (Lepidoptera: Noctuidae) to Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae). Journal of Economic Entomology, 93 (4): 1105-1108.
8. Gozel, U. & C. Gunes, 2008. Natural occurance of entomopathogenic nematodes (Rhabditia: Steinernematidae and Heterorhabditidae) in Turkey. Proceedings of the Second COST 872 Annual Meeting, 26th-29th May 2008, Postojna, Slovenia, 52pp.

9. Halawa, M.S., M.Y. El-Khloy, & T.R. Abd El-Zaher, 2007. Evaluation of entomopathogenic nematodes, a commercial bacterial bio-insecticide and the peppermint oil for the control the pink stalk borer, Sesamia cretica Led. (Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control, 17: 1-2.
10. Kaya, H.K., 1985. Entomogenous Nematodes for Insect Control in IPM Systems, 283-302. In: Biological Control in Agricultural IPM Systems (Eds: M. A. Hoy & , D. C. Herzog) Orlando, FL: Academic Press, 589 pp.
11. Kaya, H.K. & R. Gaugler, 1993. Entomopathogenic nematodes. Annual Review of Entomology 38: 181-206.
12. Klein, M.G., 1990. Efficacy Against Soil-Inhabiting Insect Pests,195-214. In: Entomopathogenic Nematodes in Biological Control (Eds: R. Gaugler & H.K. Kaya). Boca Raton, FL: CRC Press, 365 pp.
13. Koppenhöfer, A.M., 2000. Nematodes, 283-301. In: Field Manual of Techniques in Invertebrate Pathology (Eds: LA. Lacey & H.K. Kaya). Dordrecht, The Netherlands: Kluwer, 886 pp.
14. Liu, J., G. Poinar & R.E. Berry, 2000. Control of insect pest with entomopathogenic nematodes: The impact of molecular biology and phylogenetic reconstruction. Annual Review of Entomology, 45: 287-306.
15. Nguyen, K.B. & L.W. Duncan, 2002. Steinernema diaprepesi n. sp. (Rhabditida: Steinernematidae), a parasite of the citrus root weevil Diaprepes abbreviatus (L) (Coleoptera: Curculionidae). Journal of Nematology 34: 159-170. Nguyen, K.B., M. Tesfamariam, U. Gozel, R. Gaugler & B.J. Adams, 2004a. Steinernema yirgalemense n. sp. (Rhabditida: Steinernematidae) from Ethiopia. Nematology, 6: 839-856.
16. Nguyen, K.B., D.I. Shapiro-Ilan, R.J. Stuart, C.W. McCoy, R.R. James & B.J. Adams, 2004b. Heterorhabditis mexicana n. sp. (Heterorhabditidae: Rhabditida) from Tamaulipas, Mexico with morphological studies of bursa of Heterorhabditis spp. Nematology, 6: 231-244.
17. Nickle, WR., 1984. History, Development and Importance of Insect Nematology, 627-653. In: Plants and Insect Nematodes (Ed: Nickle, WR.) New York: Marcel Dekker, 925 pp.
18. Qui, L.H., Y.Y. Fang, Y. Zhou, Y. Pang & KB. Nguyen, 2004. Steinernema quangdongense sp. n. (Nematoda: Steinernematidae) a new entomopathogenic nematode from Southern China with a note on S. serratum. Zootaxa, 704: 1-20.
19. Qui, L., X. Yan, Y. Zhou, K.B. Nguyen & Y. Pang, 2005. Steinernema aciari sp. n. (Nematoda: Steinernematidae) a new entomopathogenic nematode from Guangdong China, Journal Invertebrate Pathology, 88: 58-69.
20. Smart, Jr G. C., 1995. Entomopathogenic nematodes for the biological control of insects. Supplement to the Journal of Nematology, 27: 529-534.
21. Solomon, A., I. Paperna & I. Glazer, 1999. Desiccation survival of the entomopathogenic nematode Steinernema feltiae: Induction of anhydrobiosis. Nematology, 1: 61-69.
22. Wouts, W.M., 1991. Steinernema (Neoaplectana) and Heterorhabditis species, 855-897. In: Manual of Agricultural Nematology (Ed: Nickle, WR.) New York: Marcel Dekker,1035 pp.
23. Zeren O, Güllü M, & Şimşek N., 1988. Some Biological Investigations Relating To The Control of Stalk Borer (Sesamia spp.) And European Corn Borer (Ostrinia nubilalis Hbn.) on Corn in Mediterranean Region. Proceedings of a Symposium On Corn Borers And Control Measures, 1-3 November Adana, 1-19