Study on species composition of Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) on cotton in Çukurova plain, Turkey

Yıl 2014, Cilt: 38 Sayı: 1, 43 - 50, 01.02.2014

Kamil Karut M. Bora Kaydan Steven J. Castle Cengiz Kazak M. Rifat Ulusoy

https://doi.org/10.16970/ted.41111

Cited By: 4

Öz

The whitefly Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) is one of the most important pest species on cotton in the Çukurova Plain as well as in all cotton growing areas of Turkey. Although numerous studies on population dynamics, population development and parasitism were conducted on B. tabaci in the Çukurova Plain, there has been a few studies on the species complex of B. tabaci for this region. The aims of this study were to present genetic polymorphism and species composition of B. tabaci collected from cotton fields of Çukurova Plain of Turkey between 2006 and 2011 (except 2010). Polymerase chain reaction (PCR) products were generated from a mitochondrial gene, cytochrome oxidase I (COI), and a nuclear gene region (ITS1). PCR products were purified and sequenced. Maximum likelihood (ML) and Bayesian analyses were used to estimate trees. Restriction fragment length polymorphism (RFLP) method was also applied to support results of sequence analysis and differentiating the MED and MEAM1 species of B. tabaci populations. From 51 sequences 38 of them were determined as B. tabaci MED species, and 13 of them were B. tabaci MEAM1. All samples collected in 2006 were placed in the B. tabaci MEAM1 clade with some samples collected in 2011. All PCR products were studied by RFLP method and we were able to differentiate MED and MEAM1 species of B. tabaci. MED species was determined as dominant on cotton in Çukurova Plain of Turkey

Anahtar Kelimeler

Bemisia tabaci, biotypes, cotton, sequence, PCR-RFLP, whitefly

Çukurova'da pamukta bulunan Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae)'nin tür kompleksi üzerine çalışmalar

Öz

Beyazsinek Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) Çukurova'da ve Türkiye'de pamuk üretimi yapılan diğer alanlarda, pamuğun en önemli zararlıları arasında yer almaktadır. Çukurova'da B. tabaci'nin populasyon dinamiği, populasyon gelişimi ve parazitlenme durumu ile ilgili birçok çalışma yapılmasına rağmen, B. tabaci'nin tür kompleksi hakkında çok az çalışma bulunmaktadır. Bu çalışmanın amacı 2006-2011 (2010 hariç) yılları arasında Çukurova'dan pamuk tarlalarından toplanan B. tabaci'nin genetik polimorfizmi ile tür kompleksinin ortaya çıkarılmasıdır. Polimeraz zincir reaksiyonu (PZR) ürünleri bir mitokondriyal gen olan sitokrom oksidaz I (COI) ve bir nüklear gen bölgesi olan ITS1’den elde edilmiştir. Elde edilen PZR ürünleri saflaştırılmış ve sekanslanmıştır. Filogenetik ağaç, Maximum likelihood (ML) ve Bayesian analiz yöntemleri kullanılarak oluşturulmuştur. Sekans analizi sonuçlarının desteklenmesi ve B. tabaci'nin MED (Q biyotip) ve MEAM1 (B biyotip) türlerinin belirlenmesinde Restriction Fragment Length Polymorphism (RFLP) metodu da kullanılmıştır. Toplam 51 sekans örneğinin 38 tanesi B. tabaci'nin MED türü olarak belirlenirken, 13 adedi MEAM1 olarak belirlenmiştir. 2011 yılında toplanan birkaç örnek ile beraber 2006 yılında toplanan örneklerin tamamı MEAM1 içerisinde yer almıştır. Ayrıca PZR sonucunda elde edilen ürünlerin tamamı RFLP metodu ile çalışılmış ve B. tabaci'nin MED ve MEAM1 türleri bu yöntemle de ayrılmıştır. Çukurova'da pamuk bitkisinde MED baskın tür olarak belirlenmiştir

Anahtar Kelimeler

Bemisia tabaci, biyotipler, pamuk, sekans PCR-RFLP, beyazsinek

Kaynakça

• Anonymous, 2012. Turkish Statistical Enstitute (TurkStat). http://www.turkstat.gov.tr. (04.11.2012)
• Bayhan, E., M.R. Ulusoy & J.K. Brown, 2006. Host range, distribution, and natural enemies of Bemisia tabaci ‘B biotype’ (Hemiptera: Aleyrodidae) in Turkey. Journal of Pest Science, 79: 233-240.
• Bedford, I.D., P.G. Markham, J.K. Brown & R.C. Rosell, 1994. Geminivirus transmission and biological characterization of whitefly (Bemisia tabaci) biotypes from different world regions. Annual of Applied Biology, 125: 311-325.
• Brown, J.K., D.R. Frohlich & R.C. Rosell, 1995. The sweetpotato/silverleaf whiteflies: biotypes of Bemisia tabaci (Genn.) of a species complex? Annual Reviev of Entomology, 40: 511-534.
• Boykin, L.M., R.G. Shatters, R.C. Rosell, C.L. McKenzie, R.A. Bagnall, P. De Barro & D.R. Frohlich, 2007. Global relationships of Bemisia tabaci (Hemiptera: Aleyrodidae) revealed using Bayesian analysis of mitochondrial COI DNA sequences. Molecular Phylogenetics and Evolution, 44:1306-1319
• Cervera, M.T., J.A. Cabezas, B. Simon, J.M. Martinez-Zapater, F. Beitia & J.L. Cenis, 2000. Genetic relationships among biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) based on AFLP analysis. Bulletin of Entomological Research, 90:391–96.
• Chu, D., F.H. Wan, Y.J. Zhang & J.K. Brown, 2010. Change in the biotype composition of Bemisia tabaci in Shandong Province of China from 2005 to 2008. Environmental Entomology, 39:1028-1036.
• Chu, D., C.S. Gao, P. De Barro, Y.J. Zhang, F.H. Wan & I.A. Khan, 2011. Further insights into the strange role of bacterial endosymbionts in whitefly, Bemisia tabaci: Comparison of secondary symbionts from biotypes B and Q in China. Bulletin of Entomological Research, 101:477–486.
• Chu, D., X. Hu, C. Gao, H. Zhao, R.L. Nichols & X. Li, 2012. Use of Mitochondrial Cytochrome Oxidase I Polymerase Chain Reaction-Restriction Fragment Length Polymorphism for Identifying Subclades of Bemisia tabaci Mediterranean Group. Journal of Economic Entomology, 105: 242-251.
• Dennehy, T.J., B.A. Degain, V.S. Harpold, M. Zaborac, S. Morin, J.A. Fabrick, R.L. Nichols, J.K. Brown, F.J. Byrne & X. Li, 2010. Extraordinary resistance to insecticides reveals exotic Q biotype of Bemisia tabaci (Gennadius) in the New World. Journal of Economic Entomology, 103:2174-2186.
• De Barro, P.J., F. Driver, J.W.H. Trueman & J. Curran, 2000. Phylogenetic relationships of world populations of Bemisia tabaci (Gennadius) using ribosomal ITS1. Molecular Phylogenetics and Evolution, 16: 29–36.
• De Barro, P., J.W.H. Trueman & D.R. Frohlich, 2005. Bemisia argentifolii is a race of B. tabaci (Hemiptera:Aleyrodidae): the molecular genetic differentiation of B. tabaci populations around the world. Bulletin of Entomological Research, 95:193–203.
• De Barro, P. & Z. Ahmed, 2011. Genetic networking of the Bemisia tabaci cryptic species complex reveals pattern of biological invasions. PLoS ONE 6(10): e25579.doi:10.1371/journal.pone.0025579.
• De la Rua, P., B. Simon, D. Cifuentes, C. Martinez-Mora & J.L. Cenis, 2006. New insights into the mitochondrial phylogeny of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in the Mediterranean Basin. Journal of Zoological Systematics and Evolutionary Research, 44:25–33.Dinsdale, A., L. Cook, C. Riginos, Y. M. Buckley & P. De Barro, 2010. Refined global analysis of Bemisia tabaci (Hemiptera: Sternorrhyncha: Aleyrodoiea: Aleyrodidae) mitochondrial cytochrome oxidase I to identify species level genetic boundaries. Annals of Entomological Society of America 103: 196–208.
• Erdogan, C., G.D. Moores, M.O. Gurkan, K.J. Gorman & I. Denholm, 2008. Insecticide resistance and biotype status of populatios of the tobacco whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) from Turkey. Crop Protection, 27: 600–605.
• Frohlich, D., I. Torres-Jerez, I.D. Bedford, P.G. Markham & J.K. Brown, 1999. A phylogeographic analyses of the Bemisia tabaci species complex based on mitochondrial DNA markers. Molecular Ecology, 8: 1593-1602.
• Gennadius, P. 1889. Disease of the tobacco plantations in the Trikonia. The aleurodid of tobacco. Ellenike Georgia 5: 1–3.
• Hu,J., P. De Barro, H. Zhao, J. Wang, F. Nardi & S.S.Liu, 2011. An extensive field survey combined with a phylogenetic analysis reveals rapid and widespread invasion of two alien whiteflies in China. PLoS ONE 6:1-9.
• Karut, K. & C. Kazak, 2009. Monitoring adult Eretmocerus mundus, Encarsia lutea and Bemisia tabaci with yellow sticky traps in cotton, Gossypium hirsutum. Journal of Applied Entomology, 131: 553-558.
• Karut, K & S.E. Naranjo 2009. Mortality factors affecting Bemisia tabaci populations on cotton in Turkey. Journal of Applied Entomology, 133:367-374.
• Karut, K., A.A.Y. Malik, C. Kazak, M.A. Kamberoğlu & M.R. Ulusoy, 2012. Determination of biotypes of Bemisia tabaci Gennadius 1889 (Hemiptera: Aleyrodidae) on different host plant in Adana (Balcalı) by using two different molecular methods. Turkish Journal of Entomology, 36: 93-100 (In Turkish with English abstract)
• Khasdan, V., I. Levin, A. Rosner, S. Morin, S. Kontsedalov, L. Maslenin & A.R. Horowitz, 2005. DNA markers for identifying biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) and studying population dynamics. Bulletin of Entomological Research, 95: 605-613.
• Ma, W.H., X.C. Li, T.J. Dennehy, C.L. Lei, M. Wang, B.A. Degain & R.L. Nichols, 2009. Utility of mtCOI polymerase chain reaction-restriction fragment length polymorphism in differentiating between Q and B whitefly Bemisia tabaci biotypes. Insect Science, 16: 107-114.
• Perring T.M. 2001. The Bemisia tabaci species complex. Crop Protection, 20: 725-737.
• Ronquist, F. & J.P. Huelsenbeck, 2003. Mrbayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574Stansly P. A. & S. E. Naranjo 2010. Introduction. In P. A.
• Stansly and S. E. Naranjo (Ed) Bemisia, Bionomics and Management of a Global Pest (pp VX-XVIII), New York: Springer+Business.
• Şekeroğlu, E., K. Karut, S. Yıldız & C. Kazak, 1998. Spatial distribution of preimaginal Bemisia tabaci (Homoptera: Aleyrodidae) in cotton. Proceedings of the World Cotton Research Conference 2, Athens, Greece pp. 644-647.
• Özgür, A.F. & E. Şekeroğlu, 1986. Population Development of Bemisia tabaci (Homoptera: Aleurodidae) on various cotton cultivars in Çukurova, Turkey. Agriculture, Ecosystems & Environment, 17: 83-88.
• Özgür, A.F., E. Şekeroğlu, B. Ohnesorge & H. Göçmen, 1989. Studies on the population dynamics of Bemisia tabaci Genn. (Homopt., Aleyrodidae) in Çukurova, Turkey. Journal of Applied Entomology, 107: 217-227.
• Ulusoy, M.R., J.K. Brown & E. Bayhan, 2002. The ‘B’ biotype of Bemisia tabaci now established in Turkey. European Studies Network on European Whitefly Newsletter, 13