UV radiation-induced oxidative stress and DNA damage on Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

Yıl 2015, Cilt: 39 Sayı: 1, 23 - 33, 16.03.2015

Esra Güven , Dilek Pandır , Hatice Baş

https://doi.org/10.16970/ted.06717

Cited By: 2

Öz

Exposure to ultraviolet (UV) radiation is hazardous for all organisms. UV shows numerous effects on insects. In this study, mortality effect of UV on Ephestia kuehniella Zeller, 1879 (Lepidoptera: Pyralidae) larvae was studied. The larvae were exposed to UV radiation (254 and 365 nm) at different time periods (15, 30, 45 and 60 min). After exposure, mortality rate, comet assay studies, antioxidant enzyme activities (SOD, CAT, GST and GPx), and MDA levels were investigated on the larvae. The mortality rate of E. kuehniella larvae increased with increasing exposure times. It was determined that 254 nm was more effective than 365 nm compared to the effects of short (254 nm) and long-wave (365 nm) radiations on mortality rate of E. kuehniella larvae. Likewise mortality rate, 254 nm had more potency than 365 nm on antioxidant enzyme activities and levels of MDA. According to the comet assay results, the tail DNA% and comet tail length significantly increased in all exposure times at 254 nm, but these changes were seen only 45 min and 60 min at 365 nm of UV radiation. Therefore, tail DNA% and tail lenghts of 254 nm radiation could be greater than the tail lenghts of 365 nm UV radiation. As a result, the UV radiation could be effectively as a safe pest control method and as an alternative to environmentally hazardous chemical pesticides. 

Anahtar Kelimeler

Ephestia kuehniella, UV radiation, mortality, antioxidant enzymes, comet assay

UV radyasyonun, Un güvesi, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae), larvalarında meydana getirdiği oksidatif stres ve DNA hasarının belirlenmesi

Öz

Ultraviyole (UV) radyasyona maruz kalmak tüm canlılar için tehlikelidir. UV böcekler üzerinde birçok etki göstermektedir. Bu çalışmada, Ephestia kuehniella 1879 (Lepidoptera: Pyralidae) larvaları üzerine UV’nin öldürücü etkisi çalışıldı. Larvalar UV radyasyonuna (254 ve 365 nm) farklı zaman peryotlarında (15, 30, 45 ve 60 dk.) maruz bırakıldı. Çalışma sonunda larvaların ölüm oranları, komet değeleri ve antioksidan enzim aktiviteleri (SOD, CAT, GST ve GPx) ve MDA seviyeleri araştırıldı. Işınlama süresi arttıkça E. kuehniella larvalarının ölüm oranları artmıştır. Kısa (254 nm) ve uzun (365 nm) dalga boylarını ölüm oranları üzerindeki etkilerini karşılaştırdığımızda 254 nm dalga boyunun 365 nm dalga boyundan daha etkili olduğu belirlenmiştir. Bu durum, ölüm oranı, antioksidan enzim aktivitesi ve MDA seviyesinde de tespit edilmiştir. Komet değerlendirme sonuçlarına göre kuyruk % DNA‘sı ve komet kuyruk uzunluğu 254 nm dalga boyutunun tüm uygulama zamanlarında istatistiksel olarak artarken bu değişimler 365 nm UV radyasyonun yalnızca 45. ve 60. dk. larında tespit edilmiştir. Bundan dolayı, kuyruk % DNA‘sı ve kuyruk uzunluğu 254 nm dalga boyunda 365 nm dalga boyuna göre daha fazladır. Sonuç olarak, UV radyasyon depo zararlılarının kontrolünde güvenilir bir metod ve çevreye zararlı kimyasal pestisitlere bir alternatif olarak kullanılabilir.

Anahtar Kelimeler

Ephestia kuehniella, UV radyasyon, ölüm oranı, antioksidan enzimler, komet değerlendirme

Kaynakça

• Aebi, H., 1984. Catalase in vitro. Methods in Enzymology, 105: 121-126.
• Ahmad, S., 1995. Oxidative stress from environmental pollutants. Arch. Insect Biochem. Physiol. 29: 135–157.
• Azizoglu, U., S. Yılmaz, S. Karaborklu & A. Ayvaz, 2011. Ovicidal Activity of Microwave and UV Radiations on Mediterranean Flour Moth Ephestia kuehniella Zeller, 1879 (Lepidoptera: Pyralidae). Türkiye Entomoloji Dergisi, 35: 437-446.
• Arthur, F. H., 1996. Grain protectants: current status and prospects for the future. Journal of Stored Product Research, 32: 293-302.
• Ayvaz, A. & S. Karaborklu, 2008. Effect of cold storage and different diets on Ephestia kuehniella Zeller (Lep: Pyralidae). Journal of Pest Science, 81: 57–62.
• Ayvaz, A., S. Albayrak & S. Karaborklu, 2008. Gamma Radiation Sensitivity of the Eggs, Larvae and Pupae of Indian Meal Moth Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae). Pest Management Science, 64: 505-512.
• Azizoglu, U., S. Karaborklu, S. Yılmaz, A. Ayvaz & R. Temizgul, 2010. Insecticidal activity of microwave radiation on Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) adults. EU J Institute Scie Tech 26: 323-327.
• Buyukguzel, E. & Y. Kalender, 2009. Exposure to streptomycin alters oxidative and antioxidative response in larval midgut tissues of Galleria mellonella. Pesticide Biochemistry Physiology, 94: 112–118.
• Buyukguzel, E., P. Hyršl & K. Buyukguzel, 2010. Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L. Comparative Biochemistry and Physiology Part A, 156: 176–183.
• Cohen, S. H., J. A. Saousa, J. F. Roach & J. B. Gingrich, 1975. Effects of UV irradiation on nymphs of Blattella germanica and Periplaneta americana. Journal of Economic Entomology, 68: 687-693.
• Erel, Y., N. Yazıcı, S. Ozvatan, D. Ercin & N. Cetinkaya, 2009. Detection of irradiated quail meat by using DNA comet assay and evaluation of comets by image analysis. Radiation Physics Chemistry, 78: 776–781.
• Faruki, S. I., D. R. Das, A. R. Khan & M. Khatu, 2007. Effects of ultraviolet (254nm) irradiation on egg hatching and adult emergence of the flour beetles Tribolium castaneum, T. confusum and the almond moth Cadra cautella. Journal of Insect Science, 7: 1-6.
• Felton, G. W., & C. B. Summers, 1995. Antioxidant systems in insects, Archieves of Insect Biochemistry and Physiology. 29: 187–197.
• Ghanem, I. & M. Sharma, 2007. Effect of non-ionizing radiation (UVC) on the development of Trogoderma granarium everts. Journal of Stored Product Research, 43: 362–366.
• Gruijl, F. R., H. J. Kranen & L. H. Mullenders, 2001. UV-induced DNA damage, repair, mutations and oncogenic pathways in skin cancer. Journal of Photochemistry and Photobiology B: Biology, 63: 19–27.
• Guerra, A. A., M.T. Ouye & H. R. Bullock, 1968. Effect of ultraviolet irradiation on egg hatch, subsequent larval development, and adult longevity of the tobacco budworm and the bollworm. Journal of Economic Entomology, 61: 541-542.
• Häder, D. P., H. D. Kumar, R. C. Smith & R. C. Worrest, 2007. Effects of solar UV radiation on aquatic ecosystems and interactions with climate change. Photochemical and Photobiology Science, 6: 267–285.
• Halverson, S. L., T. W. Phillips, T. S. Bigelow, G. N. Mbata & M. E. Payton, 1999. The control of various species of stored product insects with EHF energy. In: Proceeding of the Annual International Research Conference on Methyl Bromide Alternatives and Emissions Reductions. Published Abstract, pp 1-4.
• Habig, W. H., M. J. Pabst & W. B. Jakoby, 1974. Glutathione-S-transferases: the first enzymatic step in mercapturic acid formation. Journal of Biochemistry, 249: 7130-7139.
• Hallman, G. J., 2013. Control of stored products pests by ionizing radiation. Journal of Stored Product Research, 52: 36-41.
• Hansen, L. S. & K. M. V. Jensen, 2002. Effect of temperature on parasitism and host-feeding of Trichogramma turkestanica (Hymenoptera: Trichogrammatidae) on Ephestia kuehniella (Lepidoptera: Pyralidae). Journal of Economic Entomology, 95: 50–56.
• Ichihashi, M., M. Ueda, A. Budiyanto, T. Bito, M. Oka, M, Fukunaga, K. Tsuru & T. Horikawa, 2003. UV-induced skin damage. Toxicology, 189: 21-39.
• Krishnan, N.. & D. Kodrik, 2006. Antioxidant enzymes in Spodoptera littoralis (Boisduval): are they enhanced to protect gut tissues during oxidative stress. Journal of Insect Physiology, 52: 11–20.
• Liou, S. H., Y. H. Chen, C. H. Loh, T. Yang, T. N. Wu, C. J. Chen & L.L. Hsieh, 2002. The association between frequencies of mitomycin C-induced sister chromatid exchange and cancer risk in arseniasis. Toxicology Letters, 129: 237–243.
• Lowry, O. H., N. J. Rosebrough, A. L. Farr & R. J. Randall, 1951. Protein measurement with the folin phenol reagent. The Journal of Biological Chemistry, 193(1): 265-75.
• Lynn, D. E. & S. M. Ferkovich, 2004. New cell lines from Ephestia kuehniella: characterization and susceptibility to baculoviruses. Journal of Insect Science, 4: 1-5.
• Mamur, S, D. Yuzbasıoglu, F. Unal & S. Yılmaz, 2010. Does potassium sorbate induce genotoxic or mutagenic effects in lymphocytes? Toxicology In Vitro 24: 790–794.
• Marklund, S. & G. Marklund, 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry, 47: 469-474.
• Messarah, M., F. Klibet, A. Boumendjel, C. Abdennour, N, Bouzerna, M.S Boulakoud & A. E. Feki, 2012. Hepatoprotective role and antioxidant capacity of selenium on arsenic-induced liver injury in rats. Experimental Toxicology and Pathology, 64: 167–174.
• Modesto, K. A. & C. B. Martinez, 2010. Effects of roundup transorb on fish: hematology, antioxidant defenses and acetylcholinesterase activity. Chemosphere, 81:781–787.
• Mone, M. J., M. Volker, O. Nikaido, L. H. F. Mullenders, A. A. van Zeeland, P. J. Verschure, E. M. M. Manders & R. van Driel, 2001. Local UV-induced DNA damage in cell nuclei results in local transcription inhibition. EMBO Reports 21: 1013-1017.
• Mora, S., S. Demers & M. Vernet, 2000. The effects of UV radiation in the marine environment. Cambridge University Press, Cambridge.
• Mpountoukas, P., A. Vantarakis, E. Sivridis & T. Lialiaris, 2008. Cytogenetic study in cultured human lymphocytes treated with three commonly used preservatives. Food and Chemical Toxicology, l46: 2390–2393.
• Myllyperkiö, M. H., T. R. Koski, L. M. Vilpo & J. A. Vilpo, 2000. Kinetics of excision repair of UV-induced DNA damage, measured using the comet assay. Mutation Research, 448: 1–9.
• Ohkawa, H., N. Ohishi & K. Yagi, 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid rection. Analytical Biochemistry, 95: 351-358.
• Paglia, D. E. & W. N. Valentine, 1987. Studies on the quantative and qualitative characterization of glutathione peroxidase. Journal of Laboratory and Clinical Medicine, 70: 158-165.
• Patton, W. P., U. R. Chakravarthy, J. H. Davies & D. B. Archer, 1999. Comet assay of UV-induced DNA damage in retinal pigment epithelial cells. Investigate Ophthalmology and Visual Science, 40: 3268–3275.
• Rapp, A., C. Bock, H. Dittmar & K.O. Greulich, 2000. UV-A breakage sensitivity of human chromosomes as measured by COMET-FISH depends on gene density and not on the chromosome size. Journal of Photochemical Photobiology B: Biology, 56: 109–117.
• Rencuzogulları, E., B. A. Tuylu, M. Topaktas, H. B. Ila, A. Kayraldız, M. Arslan & S. Diler, 2004. Genotoxicity of Aspartame. Drug and Chemical Toxicology, 27: 257–268.
• Schöller, M. S., A. G. Prozell, A. Kirshi & C. Reichmuth, 1997. Towards biological control as a major component of integrated pest management in stored product protection. Journal of Stored Product Research, 33: 81-97.
• Tornaletti, S. & G. P. Pfeifer, 1995. UV light as a footprinting agent: Modulation of UV-induced DNA damage by transcription factors bound at the promoters of three human genes. Journal of Molecular Biology, 249: 714– 728.
• Tuncbilek A. S., U. Canpolat & F. Sumer, 2009. Suitability of rradiated and cold-stored eggs of Ephestia kuehniella (Pyralidae:Lepidoptera) and Sitotroga cerealella (Gelechidae:Lepidoptera) for stockpiling the egg-parasitoid Trichogramma evanescens (Trichogrammatidae:Hymenoptera) in diapause. Biocontrol Science and Technology, 19: 127-138.
• Yılmaz, S., F. Unal, D. Yuzbasioglu & H. Aksoy, 2008. Clastogenic effects of food additive citric acid in human peripheral lymphocytes. Cytotechnology, 56: 137–144.
• Zengin, N., D. Yuzbasioglu, F. Unal, S, Yılmaz & H. Aksoy, 2011. The evaluation of the genotoxicity of two food preservatives: Sodium benzoate and potassium benzoate. Food and Chemical Toxicology, 49: 763–769.
• Zhao, S., C. Qiu, S. Xiong & X. Cheng, 2007. A thermal lethal model of rice weevils subjected to microwave irradiation. Journal of Stored Product Research, 43: 430–434.