Normalisation values for the resonant frequency of the middle ear in rats

Yıl 2018, , 170 - 174, 18.12.2018

Şule Mıdık Belde Çulhaoğlu Selim Sermed Erbek Seyra Erbek

https://doi.org/10.32448/entupdates.508087

Öz

Objective:
In earlier studies, the resonant frequency (RF) of the middle ear has
been investigated in a number of different animals. However, no study
has so far specifically addressed the measurement of RF in rats. With
this in mind, in
our study, multifrequency tympanometry (MFT) was performed on rats
and RF value measurements were taken.
The aim was to
ascertain the normal values for rats used in our university
experiments and to provide guidance for further research to be
undertaken in this area.

Materials and Methods: For
the study, 16 male and 16 female adult Sprague Downey rats (32
animals, 64 ears) aged 10 months old and in healthy condition were
used. MFT at 226Hz frequency and above was performed on all the rats
after they had been anaesthetised. In the first instance tympanograms
were recorded using the 226Hz probe tone, following which MFT
measurements were performed. RF values for the middle ear were
determined in the range 200-2000Hz.

Results: For
male rats, the mean RF value was 426.56 (± 193.01 Hz), whilst for
female rats the mean was 496.88 (± 132.55) Hz. When the values
obtained for the male and female rats were compared, no statistically
significant difference was observed between the two groups (p=0.061).
Accordingly, the mean RF value for the rats as a whole (obtained for
64 ears) was calculated to be 461.7 (± 168.02) Hz.

Conclusion: In
this research, RF values for the middle ear of rats has been
demonstrated to be measurable. However, further research is needed
into the relationship between RF and the characteristics of the
middle ear in rats.

Anahtar Kelimeler

multifrequency tympanometry, resonant frequency, rat, normalisation

Kaynakça

• 1. Terkildsen K, Thomsen K. The influence of pressure variations on the impedance of the human ear drum. J Laryngol Otology 1959; 73: 409–418.
• 2. Holte L. Aging effects in multifrequency tympanometry. Ear Hear 1996; 17:12–18.
• 3. Iacovou E, Vlastarakos P.V, Ferekidis E, Nikolopoulos T.P. Multi-Frequency Tympanometry: Clinical Applications for the Assesment of the Middle Ear Status. Indian J Otolaryngol Head Neck Surg 2013; 65(3):283–287.
• 4. Prabhu P, Manandhar S, Nasima Ahmed B. Test-Retest Reliability of the Components of Multi-Frequency Tympanometry. J International Advanced Otology 2017; 13(1): 74-6.
• 5. Öz I. Meniere hastalığında gliserol testi ile rezonans frekans farklılığının değerlendirilmesi. Başkent Üniversitesi Sağlık Bilimleri Enstitüsü, Yüksek Lisans Tezi.2018.
• 6. Margolis RH, Van Camp KJ, Wilso RH. Multifrequency tympanometry in normal ears. J. Audiology 1985; 24: 44-53.
• 7. Lehnhardt E, Laszig R., Praxis der Audiometrie. Stuttgart, Georg Thieme, 2001.
• 8. Abou-Elhamd KE, Abd-Ellatif AE, Sultan MA. The role of multifrequency tympanometry in otitis media. Saudi Med J. 2006; Vol.27(3): 357-360.
• 9. Cole LK, Podell M, Kwochka KW. Impedance audiometry measurements in clinically normal dogs. American Journal of Veterinary Research 2000; 61: 442-5.
• 10. Shanks J, Shelton C. Basic principles and clinical applications of tympanometry. Clin Audioly. 1991; 24: 299-328.
• 11. Kiessling J. Impedanzaudiometrie in der HNO Fachpraxis. In: HNO Praxis Heute (Ganz H, Schätzle W, editörler). 5–26, Springer-Verlag, New York, 1982.
• 12. Rosowski J., Outer and Middle Ears. In: Comparatitive Hearing in Mammals (Fay R, Popper A, editör)., s172–237, New York, Springer-Verlag, 1994.
• 13. Stieve M, Mojallal H, Battmer R.D, Winter M, Lenarz T., Multifrequency tympanometry: Experimental application after implantation of ossicular replacement prosthesis in rabbits. Otology and Neurotology 2007; 28:875Y877.
• 14. Steinbach E, Vergleichende untersuchungen an gehörknöchelchen und knochentransplantaten beim kaninchen und menschen. Medizinische Fakultät Tübingen Habilitationsschrift 1973; 31–2.
• 15. Wiley TL, Cruickshanks KJ, Nondahl DM, Tweed TS. Self- reported hearing handicap and audiometric measures in older adults. Journal of the American Academy of Audiology 1999; 10: 173-179.