Effects of Food Restriction on PTGS2 and NRF2 Genes Expression Levels in Rat Testis

Öz

This study aimed to investigate the effects of food restriction on oxidative status at the molecular levels of the testis in rats. A total of 16 male Wistar rats were allocated to two groups (n=8) and fed for four weeks. The first group was control (Cont) and consumed food and water ad libitum. The second one, food-restricted group (FR) was presented half of the regular consumption. But the water was presented ad libitum. The feeding period was maintained for four weeks. At the end of the feeding period, rats were euthanized by cardiac blood sampling under anesthesia. Malondialdehyde (MDA) levels in testis tissue were determined and calculated as nmol/mg protein. In addition, Prosta-glandin-Endoperoxide Synthase 2 (PTGS2) and Nuclear factor erythroid 2–related factor 2 (NRF2) genes expression levels were determined in testis tissue. While MDA and gene expression levels were found similar in groups, a positive correlation was found between PTGS2 and NRF2 genes (r=0.629; P<0.05). The molecular regulation of oxidative sta-tus was found strongly related with PTGS2 and NRF2 genes molecular activity in testis of rats. The obtained results were shown that the feeding period and restriction rate factors were mainly responsible for the oxidative status of testis tissue.

Anahtar Kelimeler

• Food restriction
• NRF2
• oxidative stress
• PTGS2
• testis

Yem Kısıtlamasının Rat Testisinde PTGS2 ve NRF2 Genlerinin Ekspresyon Seviyelerine Etkileri

Öz

Bu çalışmanın amacı, yem kısıtlamasının rat testisinde oksidatif duruma etkilerinin moleküler seviyede belirlenme-sidir. Toplam 16 erkek Wistar rat iki gruba ayrılmıştır (n=8) ve dört hafta boyunca beslenmiştir. Birinci grup olan Kontrol (Cont) grubuna yem ve su ad libitum olarak verilmiştir. İkinci grup olan, yem kısıtlaması (FR) grubuna Cont grubunun yarısı kadar yem sunulurken su ad libitum olarak sunulmuştur. Beslenme periyodunun sonunda ratlar anestezi altında kardiyak kan alınarak ötenazi edilmiştir. Testis dokusunda Malondialdehit (MDA) seviyeleri belirlenmiş ve nmol/mg protein olarak hesaplanmıştır. Ayrıca testis dokusunda Prostaglandin-Endoperoxide Synthase 2 (PTGS2) ve Nuclear factor erythroid 2–related factor 2 (NRF2) genlerinin ekspresyon seviyeleri tespit edilmiştir. Gruplarda MDA ve gen ekspresyon seviyeleri benzer bulunurken, PTGS2 ve NRF2 genleri arasında pozitif korelasyon bulunmuştur (r=0.629; P<0.05). Rat testisinde oksidatif durumun moleküler regülasyonu, PTGS2 ve NRF2 genlerinin moleküler aktivitesi ile güçlü bir şekilde ilişkili bulunmuştur. Elde edilen sonuçlar, testis dokusunun oksidatif durumundan esas olarak beslen-me süresi ve kısıtlama oranı faktörlerinin sorumlu olduğunu göstermiştir.

Anahtar Kelimeler

• NRF2
• Oksidatif stres
• PTGS2
• testis
• yem kısıtlaması

Kaynakça

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