Rheum ribes Uygulamasının Diyabetik Ratların Karaciğer ve Böbreklerinde TLR2 ve TLR4'ün İmmunohistokimyasal Lokalizasyonu Üzerine Etkisi

Öz

Diyabetes mellitus (DM) çeşitli komplikasyonlara sebep olmaktadır. Bu komplikasyonların en önemlileri hemostaz-dan sorumlu olan karaciğer ve böbrekte meydana gelen komplikasyonlardır. Işgın (Rheum ribes, R. ribes) çeşitli hasta-lıkların yanı sıra DM’de de tedavi amaçlı kullanılmaktadır. TLR aile üyeleri birçok durumda artmaktadır. Ancak diyabetik hastalarda TLR2 ve TLR4'teki ekspresyon artışı dikkat çekmektedir. Çalışmamızda R. ribes ekstraktının diyabetteki etkilerinin yanı sıra diyabetik olgularda belirgin olarak artan TLR2 ve TLR4'ün immünohistokimyasal lokalizasyonunu incelemeyi amaçladık. DM deneklerde Streptozotosin (STZ) 50 mg/kg intraperitoneal uygulanarak oluşturuldu. R. ribes ve diyabet + R. ribes gruplarına 21 gün boyunca oral gavaj ile 200 mg/kg R. ribes ekstraktı verildi. Yapılan incelemede karaciğer dokusunda TLR2 immünreaktivitesi hepatositlerde, V. sentralislerde, sinuzoid endotellerinde; TLR4 immünre-aktivitesi V. sentralis, hepatositler ve V. interlobularislerde görüldü. Böbrekte ise TLR2 ve TLR4 immünreaktivite glome-rulus hücrelerinde, proksimal tubullerde, distal tubullerde ve medullada gözlendi. TLR2 sitoplazmik immünreaktif oldu-ğu halde TLR4 hem sitoplazmik hem de nükleerdi. Hem TLR2 hem de TLR4 için immünreaktivite diyabet grubu karaci-ğer ve böbrek dokularında yoğun olarak gözlenirken, R. ribes ekstraktı uygulanan diyabet grubu karaciğer ve böbrek dokularında immünreaktivitenin önemli ölçüde azaldığı gözlendi. Sonuç olarak diyabetik ratların karaciğer ve böbrek dokularında R. ribes bitkisinin pozitif bir etkiye sahip olduğu belirlenmiştir. Bundan dolayı çeşitli hastalıklarda da kullanı-lan R. ribes bitkisi DM hastalığı için ilaç endüstrisinde yeni tedavi yöntemlerinin geliştirilmesine katkı sağlayacağını düşünmekteyiz.

Anahtar Kelimeler

• Böbrek
• diyabetes mellitus
• immunohistokimya
• karaciğer
• Rheum ribes
• TLR2
• TLR4

Effect of Rheum ribes Application on Immunohistochemical Localizations of TLR2 and TLR4 in the Liver and Kidneys of Diabetic Rats

Öz

Diabetes mellitus (DM) causes various complications. The most important of these complications are those occurring in the liver and kidney, which are responsible for hemostasis. Rheum ribes (R. ribes) is used for treatment purposes in DM as well as various diseases. TLR family members are increased in many cases. However, the in-creased expression of TLR2 and TLR4 in diabetic patients is noteworthy. In our study, we aimed to examine the effects of R. ribes extract in diabetes as well as the immunohistochemical localization of TLR2 and TLR4, which are signifi-cantly increased in diabetic cases. Streptozotocin (STZ) was administered intraperitoneally 50 mg/kg in DM subjects. R. ribes and diabetes + R. ribes groups were given 200 mg/kg R. ribes extract by oral gavage for 21 days. In the exam-ination, TLR2 immunoreactivity in liver tissue was found in hepatocytes, central veins, and sinusoid endothelium; TLR4 immunoreactivity was seen in V. centralis, hepatocytes and V. interlobularis. In the kidney, TLR2 and TLR4 immunore-activity was observed in glomerulus cells, proximal tubules, distal tubules, and medulla. While TLR2 was cytoplasmic immunoreactive, TLR4 was both cytoplasmic and nuclear. Immunoreactivity for both TLR2 and TLR4 was observed intensely in the liver and kidney tissues of the diabetes group. But it was observed that immunoreactivity was signifi-cantly reduced in the liver and kidney tissues of the diabetic group administered R. ribes extract. As a result, it was determined that R. ribes plant has a positive effect on the liver and kidney tissues of diabetic rats. Therefore, we think that the R. ribes plant, which is also used in various diseases, will contribute to the development of new treatment methods in the pharmaceutical industry for DM disease.

Anahtar Kelimeler

• Diabetes mellitus
• immunohistochemistry
• kidney
• liver
• Rheum ribes
• TLR2
• TLR4

Kaynakça

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