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Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats

Yıl 2024, , 162 - 167, 02.12.2024
https://doi.org/10.32707/ercivet.1587330

Öz

In this study, the effects of potassium dichromate (K2Cr2O7) and boron (B, as boric acid) on oxidative stress and DNA damage in rat serum and liver were investigated. Sixty female Sprague-Dawley rats were divided into six groups of 10 animals each. The first group was kept as the control group. The second and third groups received 5 and 10 mg/kg B, respectively, the forth group received 10 mg/kg K2Cr2O7, and the fifth and sixth groups received 5 and 10 mg/kg B plus K2Cr2O7, respectively, orally for two weeks. Compared with the control groups, serum MDA levels increased (P<0.01) and TAC levels decreased (P<0.001) in the K2Cr2O7 group. Serum MDA levels decreased in the K2Cr2O7+5 and 10 mg/kg B groups, but a significant decrease was found in the K2Cr2O7+10 mg/kg B group (P<0.01). Serum TAC levels showed a numerical increase in K2Cr2O7+B groups. The liver MDA level was significantly decreased in the K2Cr2O7+10 mg/kg B group compared to the other groups (P<0.01). There was no difference in plasma 8-OHdG levels between the groups. A positive correlation was observed between liver B and Cr levels (P<0.05). In this study, serum MDA and TAC levels were negatively affected in rats administered 10 mg/kg K2Cr2O7. In contrast, administration of 10 mg/kg B to the K2Cr2O7 group had positively effected on serum and liver lipid peroxidation indicators.

Kaynakça

  • Acaröz U, İnce S, Arslan Acaröz D, Gürler Z, Demirel HH, Küçükkurt İ, Eryavuz A, Kara R, Varol N, Zhu K. Bisphenol-A induced oxidative stress, inflamma-tory gene expression, and metabolic and histopathological changes in male Wistar albino rats: protective role of boron. Toxicol Res 2019; 7(3): 503-12.
  • Acaröz U, İnce S, Arslan Acaröz D, Gürler Z, Küçük-kurt İ, Demirel HH, Arslan HO, Varol N, Zhu K. The ameliorative effects of boron against acrylamide-induced oxidative stress, inflammatory response, and metabolic changes in rats. Food Chem Toxicol 2018; 118: 745-52.
  • Anderson RA, Polansky MM. Dietary and metabolite effects on trivalent chromium retention and distribution in rats. Biol Trace Elem Res 1995; 50(2): 97-108.
  • Barceloux DG. Chromium. Clin Toxicol 1999; 37(2): 173-94.
  • Bashandy SAE, Ebaid H, Al-Tamimi J, Ahmed-Farid OAH, Omara EA, Alhazza IM. Melatonin alleviated potassium dichromate-induced oxidative stress and reprotoxicity in male rats. Hindawi BioMed Res Int2021, Article ID 3565360, 1-12.
  • Butterwick L, De Oude N, Raymond K. Safety assessment of boron in aquatic and terrestrial environments. Ecotox Environ Safe 1989; 17: 339-71.
  • Coban FK, Ince S, Kucukkurt I, Demirel HH, Hazman Ö. Boron attenuates malathion-induced oxidative stress and acetylcholinesterase inhibition in rats. Drug Chem Toxicol 2015; 38(4): 391-9.
  • Cohen MD, Kargacin B, Klein CB, Costa M. Mechanisms of chromium carcinogenicity and toxicity. Crit Rev Toxicol 1993; 23: 255-81.
  • Çakır S, Eren M, Şentürk M, Soyer Sarıca Z. The effect of boron on some biochemical parameters in experimental diabetic rats. Biol Trace Elem Res 2018; 184(1): 165-72.
  • Danadevi K, Rozati R, Saleha BB, Grover P. Geno-toxic evaluation of welders occupationally exposed to chromium and nickel using the Comet and micro-nucleus assays. Mutagenesis 2004; 19: 35-41.
  • Fedala A, Adjroud O, Abid-Essefi S, Timoumi R. Pro-tective effects of selenium and zinc against potas-sium dichromate-induced thyroid disruption, oxidati-ve stress, and DNA damage in pregnant Wistar rats. Environ Sci Pollut Res Int 2021; 28 (18): 22563-76.
  • Ince S, Keles H, Erdoğan M, Hazman Ö, Küçükkurt İ. Protective effect of boric acid against carbon tet-rachloride-induced hepatotoxicity in mice. Drug Chem Toxicol 2012; 35: 285-92.
  • Ince S, Kucukkurt I, Cigerci İH, Fidan AF, Eryavuz A. The effects of boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. J Trace Elem Med Biol 2010; 24: 161-4.
  • Ince S, Kucukkurt I, Demirel HH, Acaröz U, Arslan Acaröz D, Varol N. Boron ameliorates arsenic-induced DNA damage, proinflammatory cytokine gene expressions, oxidant/antioxidant status, and biochemical parameters in rats. J Biochem Mol Toxicol 2019; 33:e22252.
  • Ince S, Kucukkurt I, Demirel HH, Arslan Acaröz D, Abal E, Cigerci İH. Protective effects of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats. Chemosphere 2014;108:197-204.
  • Iztleuov M, Temirova G, Bashbayeva M, Komyekbay Z, Iztleuov Y, Madikhan Z, Yemzharova G. Effect of sodium tetraborate on oxidative damages in heart tissue in chromium intoxication. Biomed & Pharma-col J 2019; 12(2): 609-18.
  • Klaunig JE, Kamendulis LM. The role of oxidative stress in carcinogenesis. Annu Rev Pharmacol Toxicol 2004; 44: 239-67.
  • Küçükkurt İ, Demirel HH, Arslan Acaröz D, İnce S, Eryavuz A. Potential protective effect of boron aga-inst gentamicin-induced oxidative stress on rat tis-sues. Kocatepe Vet Derg 2017; 10(4): 300-7.
  • Küçükkurt İ, İnce S, Akbel E,Karabağ Çoban F. The effects of dietary boron compounds in supplemen-ted diet on hormonal activity and some biochemical parameters in rats. Toxicol Indust Health 2015; 31(3): 255-60.
  • Küçükkurt İ, İnce S, Demirel HH, Türkmen R, Akbel E, Çelik Y. The effects of boron on arsenic induced lipid peroxidation and antioxidant status in male and female rats. J Biochem Mol Toxicol 2015; 29(12): 564-71.
  • McDowell LR. Minerals in Animal and Human Nutri-tion. Academic Press 1992; pp. 265-370.
  • Mertz W. Chromium Occurrence and Function in Bio-logical Systems. Physicological Reviews. Washington: Walter Reed Army Medical Center, 1969; p. 163-239.
  • Mohammed NE, Saber RA. Effect of aqueous extract of damsissa (Ambrosia maritima) on the biochemical changes induced by potassium dichromate in rats. J Am Sci 2011; 7(3): 234-42.
  • National Research Council. Nutrient Requirements of Poultry. Ninth Edition, Washington DC: National Academy of Sciences Press, 1994; p. 15.
  • Navya K, Phani Kuamr G, Chandrasekhar Y, Anila-kumar KR. Evaluation of potassium dichromate (K2Cr2O7)-induced liver oxidative stress and amelio-rative effect of Picrorhiza kurroa extract in Wistar albino rat. Biol Trace Elem Res 2018; 184: 154-64.
  • Nickens KP, Patierno SR, Ceryak S. Chromium genotoxicity: a double-edged sword. Chem Biol Inte-ract 2010; 188: 276-88.
  • Nielsen FH. Biochemical and physiological consequences of boron deprivation in humans. Environ Health Perspect 1994; 102(suppl 7): 59-63.
  • Nielsen FH. Boron-An overlooked element of potential nutritional importance. Nutr Today 1988; 4-7.
  • Nielsen FH. New essential trace elements for the life sciences. Biol Trace Elem Res 1990; 26-27: 599-611.
  • Nielsen FH. Nutritional requirements for boron, silicon, vanadium, nickel, and arsenic: Current knowledge and speculation. FASEB J 1991; 5:2661-7.
  • Nordberg J, Arner ES. Reactive oxygen species, anti-oxidants, and the mammalian thioredoxin system. Free Radical Biol Med 2001; 31: 1287-312.
  • Orhan S, Ruhi Turkmen R, Demirel HH, Akosman M, Fırat F. An investigation of the effect of chlorogenic acid on potassium dichromate- induced oxidative stress in rats. Research Square (preprint) 2022; 1-27.
  • Patel G, Joshi DV, Patel BJ, Raval SH, Lambade PS. Potassium Dichromate induced genotoxicity and oxidative stress in Wistar rats (Rattus norvegicus). Indian J Vet Pathol 2016; 40(1): 42-6.
  • Patlolla AK, Barnes C, Yedjou C, Velma VR, Tchounwou PB. Oxidative Stress, DNA Damage, and antioxidant enzyme activity ınduced by hexavalent chromium in Sprague-Dawley rats. En-viron Toxicol 2009; 24(1): 66-73.
  • Pawa S, Ali S. Boron ameliorates fulminant hepatic failure by counteracting the changes associated with the oxidative stress. Chem-Biol Inter 2006; 160: 89-98.
  • Price CJ, Strong PL, Murray FJ, Goldberg MM. Blood boron concentrations in pregnant rats fed boric acid throughout gestation. Reprod Toxicol 1997; 11(6): 833-42.
  • Sarıca Soyer Z, Eren M, Şentürk M. Effect of boron on the potassium dichromate induced oxidative damage in brain tissue of Sprague Dawley rats. Pak J Zoology 2019; 51(5): 1905-10.
  • Türkez H, Geyikoğlu F, Tatar A, Keleş S, Özkan A. Effects of some boron compounds on peripheral human blood. Z Naturforsch 2007; 62: 889-96.
  • Underwood EJ, Suttle NF. The Mineral Nutrition of Livestock. Third Edition. New York: CABI Publis-hing, 1999; pp. 513-42.
  • WHO. Trace Elements in Human Nutrition and He-alth: Boron. Geneva, 1996; pp. 175-82.
  • Zayed AM, Terry N. Chromium in the environment: factors affecting biological remediation. Plant Soil 2003; 249: 139-56.

Ratlarda Potasyum Dikromat ile Bor’un Oksidatif Stres ve DNA Hasarı Üzerine Etkileri

Yıl 2024, , 162 - 167, 02.12.2024
https://doi.org/10.32707/ercivet.1587330

Öz

Bu çalışmada, sıçanlarda potasyum dikromat (K2Cr2O7) ve borun (B, borik asit olarak) serum ve karaciğer oksidatif stres ve DNA hasarı üzerine etkileri araştırıldı. Altmış dişi Sprague-Dawley sıçan, her birinde 10 hayvan bulunan altı gruba ayrıldı. Birinci grup kontrol grubu olarak tutuldu. İkinci ve üçüncü gruplara sırasıyla 5 ve 10 mg/kg B; dördüncü gruba 10 mg/kg K2Cr2O7; beş ve altıncı gruplara ise 5 ve 10 mg/kg B ile birlikte K2Cr2O7 2 hafta boyunca oral olarak verildi. Kontrol grupları ile karşılaştırıldığında K2Cr2O7 grubunda serum MDA düzeyleri artarken (P<0.01), TAC düzeyleri azaldı (P<0.001). Serum MDA düzeyleri K2Cr2O7+5 ve 10 mg/kg B gruplarında azaldı; ancak, anlamlı azalma K2Cr2O7+10 mg/kg B grubunda (P<0.01) tespit edildi. Serum TAC düzeyleri K2Cr2O7+B gruplarında sayısal artış göster-di. Karaciğer MDA düzeyi, K2Cr2O7+10 mg/kg B grubunda diğer gruplara göre anlamlı düzeyde azaldı (P<0.01). Grup-lar arasında plazma 8-OHdG düzeyleri açısından fark belirlenemedi. Karaciğer B ve Cr düzeyleri arasında pozitif korelasyon saptandı (P<0.05). Bu çalışmada 10 mg/kg K2Cr2O7 uygulanan ratlarda serum MDA ve TAC düzeyleri olumsuz etkilenirken, K2Cr2O7 grubuna 10 mg/kg B verilmesi, serum ve karaciğer lipid peroksidasyon göstergeleri üzerine olum-lu etki yaptığı söylenebilir.

Kaynakça

  • Acaröz U, İnce S, Arslan Acaröz D, Gürler Z, Demirel HH, Küçükkurt İ, Eryavuz A, Kara R, Varol N, Zhu K. Bisphenol-A induced oxidative stress, inflamma-tory gene expression, and metabolic and histopathological changes in male Wistar albino rats: protective role of boron. Toxicol Res 2019; 7(3): 503-12.
  • Acaröz U, İnce S, Arslan Acaröz D, Gürler Z, Küçük-kurt İ, Demirel HH, Arslan HO, Varol N, Zhu K. The ameliorative effects of boron against acrylamide-induced oxidative stress, inflammatory response, and metabolic changes in rats. Food Chem Toxicol 2018; 118: 745-52.
  • Anderson RA, Polansky MM. Dietary and metabolite effects on trivalent chromium retention and distribution in rats. Biol Trace Elem Res 1995; 50(2): 97-108.
  • Barceloux DG. Chromium. Clin Toxicol 1999; 37(2): 173-94.
  • Bashandy SAE, Ebaid H, Al-Tamimi J, Ahmed-Farid OAH, Omara EA, Alhazza IM. Melatonin alleviated potassium dichromate-induced oxidative stress and reprotoxicity in male rats. Hindawi BioMed Res Int2021, Article ID 3565360, 1-12.
  • Butterwick L, De Oude N, Raymond K. Safety assessment of boron in aquatic and terrestrial environments. Ecotox Environ Safe 1989; 17: 339-71.
  • Coban FK, Ince S, Kucukkurt I, Demirel HH, Hazman Ö. Boron attenuates malathion-induced oxidative stress and acetylcholinesterase inhibition in rats. Drug Chem Toxicol 2015; 38(4): 391-9.
  • Cohen MD, Kargacin B, Klein CB, Costa M. Mechanisms of chromium carcinogenicity and toxicity. Crit Rev Toxicol 1993; 23: 255-81.
  • Çakır S, Eren M, Şentürk M, Soyer Sarıca Z. The effect of boron on some biochemical parameters in experimental diabetic rats. Biol Trace Elem Res 2018; 184(1): 165-72.
  • Danadevi K, Rozati R, Saleha BB, Grover P. Geno-toxic evaluation of welders occupationally exposed to chromium and nickel using the Comet and micro-nucleus assays. Mutagenesis 2004; 19: 35-41.
  • Fedala A, Adjroud O, Abid-Essefi S, Timoumi R. Pro-tective effects of selenium and zinc against potas-sium dichromate-induced thyroid disruption, oxidati-ve stress, and DNA damage in pregnant Wistar rats. Environ Sci Pollut Res Int 2021; 28 (18): 22563-76.
  • Ince S, Keles H, Erdoğan M, Hazman Ö, Küçükkurt İ. Protective effect of boric acid against carbon tet-rachloride-induced hepatotoxicity in mice. Drug Chem Toxicol 2012; 35: 285-92.
  • Ince S, Kucukkurt I, Cigerci İH, Fidan AF, Eryavuz A. The effects of boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. J Trace Elem Med Biol 2010; 24: 161-4.
  • Ince S, Kucukkurt I, Demirel HH, Acaröz U, Arslan Acaröz D, Varol N. Boron ameliorates arsenic-induced DNA damage, proinflammatory cytokine gene expressions, oxidant/antioxidant status, and biochemical parameters in rats. J Biochem Mol Toxicol 2019; 33:e22252.
  • Ince S, Kucukkurt I, Demirel HH, Arslan Acaröz D, Abal E, Cigerci İH. Protective effects of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats. Chemosphere 2014;108:197-204.
  • Iztleuov M, Temirova G, Bashbayeva M, Komyekbay Z, Iztleuov Y, Madikhan Z, Yemzharova G. Effect of sodium tetraborate on oxidative damages in heart tissue in chromium intoxication. Biomed & Pharma-col J 2019; 12(2): 609-18.
  • Klaunig JE, Kamendulis LM. The role of oxidative stress in carcinogenesis. Annu Rev Pharmacol Toxicol 2004; 44: 239-67.
  • Küçükkurt İ, Demirel HH, Arslan Acaröz D, İnce S, Eryavuz A. Potential protective effect of boron aga-inst gentamicin-induced oxidative stress on rat tis-sues. Kocatepe Vet Derg 2017; 10(4): 300-7.
  • Küçükkurt İ, İnce S, Akbel E,Karabağ Çoban F. The effects of dietary boron compounds in supplemen-ted diet on hormonal activity and some biochemical parameters in rats. Toxicol Indust Health 2015; 31(3): 255-60.
  • Küçükkurt İ, İnce S, Demirel HH, Türkmen R, Akbel E, Çelik Y. The effects of boron on arsenic induced lipid peroxidation and antioxidant status in male and female rats. J Biochem Mol Toxicol 2015; 29(12): 564-71.
  • McDowell LR. Minerals in Animal and Human Nutri-tion. Academic Press 1992; pp. 265-370.
  • Mertz W. Chromium Occurrence and Function in Bio-logical Systems. Physicological Reviews. Washington: Walter Reed Army Medical Center, 1969; p. 163-239.
  • Mohammed NE, Saber RA. Effect of aqueous extract of damsissa (Ambrosia maritima) on the biochemical changes induced by potassium dichromate in rats. J Am Sci 2011; 7(3): 234-42.
  • National Research Council. Nutrient Requirements of Poultry. Ninth Edition, Washington DC: National Academy of Sciences Press, 1994; p. 15.
  • Navya K, Phani Kuamr G, Chandrasekhar Y, Anila-kumar KR. Evaluation of potassium dichromate (K2Cr2O7)-induced liver oxidative stress and amelio-rative effect of Picrorhiza kurroa extract in Wistar albino rat. Biol Trace Elem Res 2018; 184: 154-64.
  • Nickens KP, Patierno SR, Ceryak S. Chromium genotoxicity: a double-edged sword. Chem Biol Inte-ract 2010; 188: 276-88.
  • Nielsen FH. Biochemical and physiological consequences of boron deprivation in humans. Environ Health Perspect 1994; 102(suppl 7): 59-63.
  • Nielsen FH. Boron-An overlooked element of potential nutritional importance. Nutr Today 1988; 4-7.
  • Nielsen FH. New essential trace elements for the life sciences. Biol Trace Elem Res 1990; 26-27: 599-611.
  • Nielsen FH. Nutritional requirements for boron, silicon, vanadium, nickel, and arsenic: Current knowledge and speculation. FASEB J 1991; 5:2661-7.
  • Nordberg J, Arner ES. Reactive oxygen species, anti-oxidants, and the mammalian thioredoxin system. Free Radical Biol Med 2001; 31: 1287-312.
  • Orhan S, Ruhi Turkmen R, Demirel HH, Akosman M, Fırat F. An investigation of the effect of chlorogenic acid on potassium dichromate- induced oxidative stress in rats. Research Square (preprint) 2022; 1-27.
  • Patel G, Joshi DV, Patel BJ, Raval SH, Lambade PS. Potassium Dichromate induced genotoxicity and oxidative stress in Wistar rats (Rattus norvegicus). Indian J Vet Pathol 2016; 40(1): 42-6.
  • Patlolla AK, Barnes C, Yedjou C, Velma VR, Tchounwou PB. Oxidative Stress, DNA Damage, and antioxidant enzyme activity ınduced by hexavalent chromium in Sprague-Dawley rats. En-viron Toxicol 2009; 24(1): 66-73.
  • Pawa S, Ali S. Boron ameliorates fulminant hepatic failure by counteracting the changes associated with the oxidative stress. Chem-Biol Inter 2006; 160: 89-98.
  • Price CJ, Strong PL, Murray FJ, Goldberg MM. Blood boron concentrations in pregnant rats fed boric acid throughout gestation. Reprod Toxicol 1997; 11(6): 833-42.
  • Sarıca Soyer Z, Eren M, Şentürk M. Effect of boron on the potassium dichromate induced oxidative damage in brain tissue of Sprague Dawley rats. Pak J Zoology 2019; 51(5): 1905-10.
  • Türkez H, Geyikoğlu F, Tatar A, Keleş S, Özkan A. Effects of some boron compounds on peripheral human blood. Z Naturforsch 2007; 62: 889-96.
  • Underwood EJ, Suttle NF. The Mineral Nutrition of Livestock. Third Edition. New York: CABI Publis-hing, 1999; pp. 513-42.
  • WHO. Trace Elements in Human Nutrition and He-alth: Boron. Geneva, 1996; pp. 175-82.
  • Zayed AM, Terry N. Chromium in the environment: factors affecting biological remediation. Plant Soil 2003; 249: 139-56.
Toplam 41 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Biyokimya
Bölüm Araştırma Makalesi
Yazarlar

Meryem Eren 0000-0003-1339-0493

Meryem Şentürk 0000-0002-3763-8310

Yayımlanma Tarihi 2 Aralık 2024
Gönderilme Tarihi 1 Nisan 2024
Kabul Tarihi 10 Temmuz 2024
Yayımlandığı Sayı Yıl 2024

Kaynak Göster

APA Eren, M., & Şentürk, M. (2024). Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 21(3), 162-167. https://doi.org/10.32707/ercivet.1587330
AMA Eren M, Şentürk M. Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats. Erciyes Üniv Vet Fak Derg. Aralık 2024;21(3):162-167. doi:10.32707/ercivet.1587330
Chicago Eren, Meryem, ve Meryem Şentürk. “Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 21, sy. 3 (Aralık 2024): 162-67. https://doi.org/10.32707/ercivet.1587330.
EndNote Eren M, Şentürk M (01 Aralık 2024) Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 21 3 162–167.
IEEE M. Eren ve M. Şentürk, “Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats”, Erciyes Üniv Vet Fak Derg, c. 21, sy. 3, ss. 162–167, 2024, doi: 10.32707/ercivet.1587330.
ISNAD Eren, Meryem - Şentürk, Meryem. “Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 21/3 (Aralık 2024), 162-167. https://doi.org/10.32707/ercivet.1587330.
JAMA Eren M, Şentürk M. Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats. Erciyes Üniv Vet Fak Derg. 2024;21:162–167.
MLA Eren, Meryem ve Meryem Şentürk. “Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, c. 21, sy. 3, 2024, ss. 162-7, doi:10.32707/ercivet.1587330.
Vancouver Eren M, Şentürk M. Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats. Erciyes Üniv Vet Fak Derg. 2024;21(3):162-7.