Comparative Pathological Evaluation of Poppy Oil and Salicylic Pomade in Bovine Dermatophytosis
Yıl 2025,
Cilt: 22 Sayı: 3, 233 - 243, 26.12.2025
Şaban Yönter
,
Zafer Özyıldız
,
Onur Çelikoğlu
Öz
This study evaluated the efficacy of poppy oil in the treatment of bovine dermatophytosis in comparison with salicylic pomade. A total of 21 animals, divided into three groups of seven each, were used in the study. Skin scrapings were collected for mycological examination from Holstein cattle with clinical fungal infections on a farm. Subsequently, poppy oil was applied topically to one group, while salicylic pomade was applied to another group for 14 days. The control group did not receive any treatment other than skin scraping. After recording the macroscopic findings for all groups, skin biopsies were collected from the lesion areas on the 1st, 7th, and 14th days for histopathological and immunohistochemical examination. The presence of the causative agent in the lesioned tissues was demonstrated using Periodic Acid-Schiff (PAS) methods. The results showed that poppy oil was effective in treating bovine dermatophytosis, similar to the salicylic pomade group. It was concluded that poppy oil could serve as an alternative therapy in veterinary medicine.
Etik Beyan
This study was carried out after the animal experiment was approved Burdur Mehmet Akif Ersoy University Animal Experiments Local Ethics Committee (Decision number: 625).
Destekleyen Kurum
his research has been supported within the content of the project no 0646-YL-20 by Burdur Mehmet Akif Ersoy University Scientific Research Projects Coordination Office
Proje Numarası
0646-YL-20
Kaynakça
-
Almeida F, Rodrigues ML, Coelho C. The still underestimated problem of fungal diseases worldwide. Front Microbiol 2019; 1(10): 214.
-
Bagdas D, Cam Etoz B, Inan Ozturkoglu S. Effects of systemic chlorogenic acid on random-pattern dorsal skin flap survival in diabetic rats. Biol Pharm Bull 2014; 37(3): 361-70.
-
Biner MM. Sıçanda insizyon yara modellerinde domuz yağı ile mupirosin pomadın yara iyileşmesi etkilerinin karşılaştırılması, Doktora tezi, Selcuk Üniv Sağ Bil Enst, Konya 2023; s:118.
-
Bozan B, Temelli F. Extraction of poppy seed oil using supercritical CO2. J Food Sci 2003; 68(2): 422-6.
-
Chaudhry NMA, Tariq P. In vitro antibacterial activities of kalonji, cumin and poppy seed. Pak J Bot 2008; 40(1): 461.
-
Chokoeva AA, Wollina U; Lotti T, Maximov GK, Lozev I, Tchernev G. Bir Bulgar çocuğunda psoriasiform dermatofitoz. Maced J Med Sci 2018; 6:118-9.
-
Freedberg IM, Tomic-Canic M, Komine M, Blumenberg M. Keratins and the keratinocyte activation cycle. J Invest Dermatol 2001; 116(5): 633-40.
-
Ghannoum M. Azole resistance in dermatophytes: prevalence and mechanism of action. J Am Podiatr Med Assoc 2016; 106(1): 79-86.
-
Gultepe A. Papaver Somniferum L. Çiçeklerinin Esansiyel Yağ İçeriği, Antimikrobiyal ve Antifungal Özelliklerinin Belirlenmesi. Yüksek lisans tezi, Afyon Kocatepe Üniv Fen Bil Enst, Afyonkarahisar 2013; s. 7-9.
-
Gupta A, Kumar P. Assessment of the histological state of the healing wound. Plast Aesthet Res 2015; 2(5): 239-82.
-
Haziroglu R, Milli HÜ. Veteriner Patoloji. 2. Baskı. Ankara: Medipres Yayınevi 2000.
-
Hunt RD, Jones TC, King NW. Veterinary Pathology. 6rd edn. New York: Lippincott Williams & Wilkins 1997.
-
Jamadagni P, Jamadagni S, Mukherjee K, Upadhyay S, Gaidhani S, Hazra J. Experimental and histopathological observation scoring methods for evaluation of wound healing properties of Jatyadi Ghrita. AYU 2016; 37(3): 222-67
-
Koga H, Nanjoh Y, Kaneda H, Yamaguçi H, Tsuboi R. Short-term therapy with luliconazole, a novel topical antifungal ımidazole, in guinea pig models of Tinea corporis and Tinea pedis. Antimicrob Agents Chemother 56.
-
Moll R, Divo M, Langbein L. The human keratins: Biology and pathology. Histochem Cell Biol 2008; 129(6): 705–33.
Ozcan MM, Atalay Ç. Determination of seed and oil properties of some poppy (Papaver somniferum L.) varieties. Grasas Aceites 2006; 57(2): 169-74.
-
Paksoy N, Ozcelik M, Erkılıc EE, Buyuk F, Ogun M, Kirmizigul AH. Kars yöresindeki dermatofitozisli sığırlarda serum bakır, çinko ve mangan seviyeleri. Ataturk Univ J Vet Sci 2013; 8(3): 210-5.
-
Persinoti GF, Martinez DA, Li W, Dogen A, Billmyre Blake R, Avarette A, Goldberg JM, Shea T, Genc T, Zeng Q, Oliver BG, Barton R, Metin B, Polat SH, İlkit M, Graser Y, Martinez-Rossi NM, Beyaz TC, Heitman J, Cuomo CA. Whole-genome analysis illustrates global clonal population structure of the ubiquitous dermatophyte pathogen trichophyton rubrum. Genetics 2018; 208(4): 1657-69.
-
Rudramurthy SM, Shankarnarayan SA, Dogra S, Shaw D, Mushtaq K, Paul RA, Narang T, Chakrabarti A. Mutation in the squalene epoxidase gene of trichophyton interdigitale and trichophyton rubrum associated with allylamine resistance. Antimicrob Agents Chemother 2018; 62(5): e02522-17.
-
Singh BR, Singh V, Ebibeni N, Singh RK. Antifungal and wound healing activity of neem oil: a review. Int J Pharm Sci Res 2017; 8(2): 1000-8.
-
Weitzman I, Summerbell RC. The dermatophytes. Clin Microbiol Rev 1995; 8(2): 240-59.
-
Yarsan E, Durgut R. Veteriner hekimliğinde deri hastalıkları ve sağaltım. 1. Baskı. Ankara: Medisan Yayınevi 2002.
Sığır Dermatofitozisinde Haşhaş Yağı ve Salisilik Pomadın Karşılaştırmalı Patolojik Değerlendirilmesi
Yıl 2025,
Cilt: 22 Sayı: 3, 233 - 243, 26.12.2025
Şaban Yönter
,
Zafer Özyıldız
,
Onur Çelikoğlu
Öz
Bu çalışmada sığırlarda dermatofitozisin tedavisinde haşhaş yağı (poppy oil) kullanımının etkinliği pomad salisilik ile karşılaştırmalı olarak değerlendirildi. Çalışmada her grupta yedi hayvan olacak şekilde toplamda yirmi bir hayvan kullanıldı. Klinik olarak derilerinde mantar enfeksiyonu bulunan bir işletmedeki holstein ırkı sığırlardan deri kazıntısı alınarak mikolojik yoklama yapıldı. Sonrasında on dört gün süreyle bir gruba haşhaş yağı, diğer gruba ise pomad salisilik topikal olarak uygulandı. Kontrol grubu hayvanlara ise deri kazıntısı haricinde herhangi bir uygulama yapılmadı. Tüm gruplardaki hayvanların makroskobik bulguları kaydedildikten sonra lezyonlu bölgelerinden birinci, yedinci ve ondördüncü günlerde deri biyopsisi alınarak histopatolojik ve immunohistokimyasal olarak incelendi. Lezyonlu bölgelerdeki etken varlığı, Peroidic acid-Schiff (PAS) yöntemi ile gösterildi. Çalışma sonunda haşhaş yağı grubunun sığır dermatofitozisinin tedavisinde etkili olduğu, pomat salisilik grubundaki tedavi sonuçlarıyla benzerlik gösterdiği ve alternatif olarak veteriner sağlığında kullanılabileceği ortaya kondu.
Etik Beyan
This study was carried out after the animal experiment was approved Burdur Mehmet Akif Ersoy University Animal Experiments Local Ethics Committee (Decision number: 625).
Destekleyen Kurum
This research has been supported within the content of the project no 0646-YL-20 by Burdur Mehmet Akif Ersoy Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü.
Proje Numarası
0646-YL-20
Kaynakça
-
Almeida F, Rodrigues ML, Coelho C. The still underestimated problem of fungal diseases worldwide. Front Microbiol 2019; 1(10): 214.
-
Bagdas D, Cam Etoz B, Inan Ozturkoglu S. Effects of systemic chlorogenic acid on random-pattern dorsal skin flap survival in diabetic rats. Biol Pharm Bull 2014; 37(3): 361-70.
-
Biner MM. Sıçanda insizyon yara modellerinde domuz yağı ile mupirosin pomadın yara iyileşmesi etkilerinin karşılaştırılması, Doktora tezi, Selcuk Üniv Sağ Bil Enst, Konya 2023; s:118.
-
Bozan B, Temelli F. Extraction of poppy seed oil using supercritical CO2. J Food Sci 2003; 68(2): 422-6.
-
Chaudhry NMA, Tariq P. In vitro antibacterial activities of kalonji, cumin and poppy seed. Pak J Bot 2008; 40(1): 461.
-
Chokoeva AA, Wollina U; Lotti T, Maximov GK, Lozev I, Tchernev G. Bir Bulgar çocuğunda psoriasiform dermatofitoz. Maced J Med Sci 2018; 6:118-9.
-
Freedberg IM, Tomic-Canic M, Komine M, Blumenberg M. Keratins and the keratinocyte activation cycle. J Invest Dermatol 2001; 116(5): 633-40.
-
Ghannoum M. Azole resistance in dermatophytes: prevalence and mechanism of action. J Am Podiatr Med Assoc 2016; 106(1): 79-86.
-
Gultepe A. Papaver Somniferum L. Çiçeklerinin Esansiyel Yağ İçeriği, Antimikrobiyal ve Antifungal Özelliklerinin Belirlenmesi. Yüksek lisans tezi, Afyon Kocatepe Üniv Fen Bil Enst, Afyonkarahisar 2013; s. 7-9.
-
Gupta A, Kumar P. Assessment of the histological state of the healing wound. Plast Aesthet Res 2015; 2(5): 239-82.
-
Haziroglu R, Milli HÜ. Veteriner Patoloji. 2. Baskı. Ankara: Medipres Yayınevi 2000.
-
Hunt RD, Jones TC, King NW. Veterinary Pathology. 6rd edn. New York: Lippincott Williams & Wilkins 1997.
-
Jamadagni P, Jamadagni S, Mukherjee K, Upadhyay S, Gaidhani S, Hazra J. Experimental and histopathological observation scoring methods for evaluation of wound healing properties of Jatyadi Ghrita. AYU 2016; 37(3): 222-67
-
Koga H, Nanjoh Y, Kaneda H, Yamaguçi H, Tsuboi R. Short-term therapy with luliconazole, a novel topical antifungal ımidazole, in guinea pig models of Tinea corporis and Tinea pedis. Antimicrob Agents Chemother 56.
-
Moll R, Divo M, Langbein L. The human keratins: Biology and pathology. Histochem Cell Biol 2008; 129(6): 705–33.
Ozcan MM, Atalay Ç. Determination of seed and oil properties of some poppy (Papaver somniferum L.) varieties. Grasas Aceites 2006; 57(2): 169-74.
-
Paksoy N, Ozcelik M, Erkılıc EE, Buyuk F, Ogun M, Kirmizigul AH. Kars yöresindeki dermatofitozisli sığırlarda serum bakır, çinko ve mangan seviyeleri. Ataturk Univ J Vet Sci 2013; 8(3): 210-5.
-
Persinoti GF, Martinez DA, Li W, Dogen A, Billmyre Blake R, Avarette A, Goldberg JM, Shea T, Genc T, Zeng Q, Oliver BG, Barton R, Metin B, Polat SH, İlkit M, Graser Y, Martinez-Rossi NM, Beyaz TC, Heitman J, Cuomo CA. Whole-genome analysis illustrates global clonal population structure of the ubiquitous dermatophyte pathogen trichophyton rubrum. Genetics 2018; 208(4): 1657-69.
-
Rudramurthy SM, Shankarnarayan SA, Dogra S, Shaw D, Mushtaq K, Paul RA, Narang T, Chakrabarti A. Mutation in the squalene epoxidase gene of trichophyton interdigitale and trichophyton rubrum associated with allylamine resistance. Antimicrob Agents Chemother 2018; 62(5): e02522-17.
-
Singh BR, Singh V, Ebibeni N, Singh RK. Antifungal and wound healing activity of neem oil: a review. Int J Pharm Sci Res 2017; 8(2): 1000-8.
-
Weitzman I, Summerbell RC. The dermatophytes. Clin Microbiol Rev 1995; 8(2): 240-59.
-
Yarsan E, Durgut R. Veteriner hekimliğinde deri hastalıkları ve sağaltım. 1. Baskı. Ankara: Medisan Yayınevi 2002.