Araştırma Makalesi
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Yarpuz (Mentha pulegium L.) Kuru Otunun Geleneksel Kültür Kaba Yemler ile in vitro Rumen Fermentasyon Değişkenlerinin Karşılaştırılması

Yıl 2025, Cilt: 22 Sayı: 3, 145 - 152, 26.12.2025
https://doi.org/10.32707/ercivet.1684585

Öz

Bu çalışmanın amacı, aromatik bir yem olan pennyroyal (Mentha pulegium L.), korunga ve geleneksel yem bitkilerinin (yonca ve mısır silajı) in vitro fermantasyon sıvısının toplam gaz üretimi, metan üretimi, in vitro metabolik enerji (ME) ve bireysel kısa zincirli yağ asitlerinin etkilerini değerlendirmektir. Ruminal fermantasyona uygun olarak in vitro gaz üretimi gerçekleştirilmiş ve 24 saatlik inkübasyon sonunda üretilen toplam gaz miktarı kaydedilmiştir. Kızılötesi gaz analiz cihazı ile in vitro toplam gaz metanındaki metan hacmi (ml) tespit edildi. Fermentasyon sıvısı, düz kısa zincirli yağ asitleri (SCFA; asetik, bütirik ve propiyonik asitler) ve dallanmış kısa zincirli yağ asitleri (BSCFA) açısından analiz edildi. Bu çalışmada korunganın in vitro toplam gaz üretimi yarpuzdan daha yüksek bulunmuştur (P>0.05). Yoncanın CH4 (ml/0.2 g KM) üretimi (6.09 ml/0.2 g KM) diğer kaba yemlerden daha yüksekti. Mısır silajının CH4 üretimi (4.02 ml/0.2 g KM) diğer kaba yemlere göre daha düşüktü. Korunga ve yoncanın ME(MJ/kg) değeri benzer olup mısır silajı diğerlerine göre daha düşüktü. Yarpuz kuru otu için in vitro rumen fermantasyon sıvısının asetik asit (AA) konsantrasyonu, yonca otu ve mısır silajına benzer (P>0.05), korunga otundan daha düşük (P<0.05) idi. Yarpuz kuru otu için in vitro rumen fermantasyon sıvısının propiyonik asit (PA) konsantrasyonu diğer kaba yemlerden daha düşüktü (P<0.05). Rumen fermantasyonunda bütirik asit (BA) konsantrasyonu yonca ve korungada yarpuz ve mısır silajına göre daha yüksekti. Bu çalışma, yarpuz otunun in vitro rumen fermentasyonu sırasında benzer asetik asit düzeyleriyle birlikte düşük PA ve metan üretimi sergileyerek, geleneksel yem bitkilerine kıyasla farklı bir fermentasyon profiline sahip olduğunu ortaya koymaktadır.

Kaynakça

  • Ando S, Nishida T, Ishida M, Hosoda K, Bayaru E. Effect of peppermint feeding on the digestibility, ruminal fermentation and protozoa. Livest Prod Sci 2003; 82(2-3): 245-48.
  • Benetel G, Silva TS, Fagundes GM, Welter KC, Melo FA, Lobo AAG, Muir JP, Bueno ICS. Essential oils as in vitro ruminal fermentation manipulators to mitigate methane emission by beef cattle grazing tropical grasses. Molecules 2022; 27(7): 2227.
  • Blümmel M, Makkar HPS, Becker K. In vitro gas production- a technique revisied. J Anim Physiol Anim Nutr 1997; 77(1-5): 24-34.
  • Boukhebti H, Chaker AN, Belhadj H, Sahli F, Ramdhani M, Laouer H, Harzallah D. Chemical composition and antibacterial activity of Mentha pulegium L. and Mentha spicata L. essential oils. Pharm Lett 2011; 3(4): 26775.
  • Ersahince AC, Kara K. Nutrient composition and in vitro digestion parameters of Jerusalem artichoke (Helianthus tuberosus L.) herbage at different maturity stages in horse and ruminant. J Anim Feed Sci 2017; 26(3): 213-25.
  • Franz Vienna CH, BauerGraz R, Carle Hohenheim R, Tedesco Milano D, Tubaro Trieste A, Zitterl-Eglseer K. Study on the assessment of plants/herbs, plant/herb extracts and their naturally or synthetically produced components as ‘additives’ for use in animal production. EFSA Supporting Publications 2007; 4(4): 297.
  • Kara K, Aktuğ E, Çağrı A, Güçlü BK, Baytok E. Formik asitin in vitro rumen fermentasyonu ve metan üretimine etkisi. TURJFAS 2015; 3(11): 856-60.
  • Kara K, Ozkaya S, Baytok E, Guclu BK, Aktug E, Erbas S. Effect of phenological stage on nutrient composition, in vitro fermentation and gas production kinetics of Plantago lanceolate herbage. Vet Med Czech 2018; 63(6): 251-60.
  • Kara K, Pirci G. Immunity, rumen metagenomics, ruminal variables, and growth performance of calves fed milk with sage (Salvia officinalis) essential oil. Trop Anim Health Prod 2023; 56(1): 27.
  • Kara K, Yilmaz S, Önel SE, Özbilgin A. Effects of plantago species herbage and silage on in vitro ruminal fermentation and microbiome. Ital J Anim Sci 2022; 21(1): 1569-83.
  • Kara K. Nutrient matter, fatty acids, in vitro gas production and digestion of herbage and silage quality of yellow sweet clover (Melilotus officinalis L.) at different phenological stages. J Anim Feed Sci 2021; 30(2): 128-40.
  • Kara K, Yilmaz S, Kocaoğlu Güçlü B, Demir S. In vitro ruminal fermentation, core nutrient, fatty acids and mineral matter of pennyroyal (Mentha pulegium L.) herbage at different phenological stages. Vet Med Sci 2024; 10(2): 1397.
  • Menke HH, Steingass H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Dev 1988; 28: 7-55.
  • Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor. J Agr Sci 1979; 93: 217-22.
  • NRC (National Research Council). Nutrient requirements of dairy cattle. National Academies Press, 2001.
  • Onel S E, Aksu T, Kara K, Aksu D S. The effect of Origanum syriacum L. extract and carvacrol on the in vitro digestion, estimated digestion values, ammonia and organic acid concentrations in the fermentation fluid of lucerne herbage. Vet Med 2022; 67(6): 309.
  • Ölmez M, Makav M. The effect of pennyroyal (Mentha pulegium L.) on growth performance and some serum biochemical parameters in New Zealand rabbits. Van Vet J 2021; 32(3): 119-24.
  • Pandur E, Micalizzi G, Mondello L, Horváth A, Sipos K, Horváth G. Antioxidant and anti-inflammatory effects of thyme (Thymus vulgaris L.) essential oils prepared at different plant phenophases on Pseudomonas aeruginosa LPS-activated THP-1 macrophages. Antioxidants 2022; 11(7): 1330.
  • Pastorelli G, Serra V, Vannuccini C, Attard E. Opuntia spp. as alternative fodder for sustainable livestock production. Animals 2022; 12(13): 1597.
  • Terrett OM, Dupree P. Covalent interactions between lignin and hemicelluloses in plant secondary cellwalls. Curr Opin Biotechnol 2019; 56: 97-104.
  • Vakili AR, Khorrami B, Mesgaran MD, Parand E. The effects of thyme and cinnamon essential oils on performance, rumen fermentation and blood metabolites in Holstein calves consuming high concentrate diet. Asian-Australas J Anim Sci 2013; 26(7): 935-44.

Comparison of in vitro Ruminal Fermentation Variables of Pennyroyal (Mentha pulegium L.) Herbage with Conventional Forages

Yıl 2025, Cilt: 22 Sayı: 3, 145 - 152, 26.12.2025
https://doi.org/10.32707/ercivet.1684585

Öz

The aim of this study is to evaluate the effects of on total gas production, methane production, in vitro metabolic energy (ME) and the individually short chain fatty acids (SCFA) of the in vitro fermentation fluid of pennyroyal (Mentha pulegium L.), an aromatic forage, and sainfoin, and conventional forages (lucerne and corn silage). In vitro gas production was carried out in accordance with ruminal fermentation, and the total amount of gas produced at the end of 24 hours of incubation was recorded. The methane volume (ml) in the in vitro total gas methane was measured using an infrared gas analyser the fermentation fluid was analysed for straight short-chain fatty acids (SCFA) (acetic, butyric, and propionic acids) and branched short-chain fatty acids (BSCFA). In the present study, the in vitro total gas production of sainfoin was higher than that of pennyroyal (P>0.05). CH4 (ml/0.2g) production (6.09 ml/0.2g) of lucerne was higher than that of other forages. CH4 production (4.02 ml/0.2g) of corn silage was lower than that of other forages. ME (MJ/kg) values of sainfoin and lucerne were similar, and corn silage was lower than the others. The acetic acid (AA) concentration of in vitro rumen fermentation fluid for pennyroyal herbage did not differ from that of lucerne herbage and corn silage (P>0.05), it was lower than that of sainfoin herbage (P<0.05). The propionic acid (PA) concentration of in vitro rumen fermentation fluid for pennyroyal herbage was lower than that of other forages (P<0.05). The butyric acid (BA) concentration in the rumen fermentation was higher in lucerne and sainfoin than pennyroyal and corn silage. This study reveals that pennyroyal exhibits a distinct fermentation profile compared to conventional forages, characterized by similar AA levels but lower PA and methane production during in vitro rumen fermentation.

Kaynakça

  • Ando S, Nishida T, Ishida M, Hosoda K, Bayaru E. Effect of peppermint feeding on the digestibility, ruminal fermentation and protozoa. Livest Prod Sci 2003; 82(2-3): 245-48.
  • Benetel G, Silva TS, Fagundes GM, Welter KC, Melo FA, Lobo AAG, Muir JP, Bueno ICS. Essential oils as in vitro ruminal fermentation manipulators to mitigate methane emission by beef cattle grazing tropical grasses. Molecules 2022; 27(7): 2227.
  • Blümmel M, Makkar HPS, Becker K. In vitro gas production- a technique revisied. J Anim Physiol Anim Nutr 1997; 77(1-5): 24-34.
  • Boukhebti H, Chaker AN, Belhadj H, Sahli F, Ramdhani M, Laouer H, Harzallah D. Chemical composition and antibacterial activity of Mentha pulegium L. and Mentha spicata L. essential oils. Pharm Lett 2011; 3(4): 26775.
  • Ersahince AC, Kara K. Nutrient composition and in vitro digestion parameters of Jerusalem artichoke (Helianthus tuberosus L.) herbage at different maturity stages in horse and ruminant. J Anim Feed Sci 2017; 26(3): 213-25.
  • Franz Vienna CH, BauerGraz R, Carle Hohenheim R, Tedesco Milano D, Tubaro Trieste A, Zitterl-Eglseer K. Study on the assessment of plants/herbs, plant/herb extracts and their naturally or synthetically produced components as ‘additives’ for use in animal production. EFSA Supporting Publications 2007; 4(4): 297.
  • Kara K, Aktuğ E, Çağrı A, Güçlü BK, Baytok E. Formik asitin in vitro rumen fermentasyonu ve metan üretimine etkisi. TURJFAS 2015; 3(11): 856-60.
  • Kara K, Ozkaya S, Baytok E, Guclu BK, Aktug E, Erbas S. Effect of phenological stage on nutrient composition, in vitro fermentation and gas production kinetics of Plantago lanceolate herbage. Vet Med Czech 2018; 63(6): 251-60.
  • Kara K, Pirci G. Immunity, rumen metagenomics, ruminal variables, and growth performance of calves fed milk with sage (Salvia officinalis) essential oil. Trop Anim Health Prod 2023; 56(1): 27.
  • Kara K, Yilmaz S, Önel SE, Özbilgin A. Effects of plantago species herbage and silage on in vitro ruminal fermentation and microbiome. Ital J Anim Sci 2022; 21(1): 1569-83.
  • Kara K. Nutrient matter, fatty acids, in vitro gas production and digestion of herbage and silage quality of yellow sweet clover (Melilotus officinalis L.) at different phenological stages. J Anim Feed Sci 2021; 30(2): 128-40.
  • Kara K, Yilmaz S, Kocaoğlu Güçlü B, Demir S. In vitro ruminal fermentation, core nutrient, fatty acids and mineral matter of pennyroyal (Mentha pulegium L.) herbage at different phenological stages. Vet Med Sci 2024; 10(2): 1397.
  • Menke HH, Steingass H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Dev 1988; 28: 7-55.
  • Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor. J Agr Sci 1979; 93: 217-22.
  • NRC (National Research Council). Nutrient requirements of dairy cattle. National Academies Press, 2001.
  • Onel S E, Aksu T, Kara K, Aksu D S. The effect of Origanum syriacum L. extract and carvacrol on the in vitro digestion, estimated digestion values, ammonia and organic acid concentrations in the fermentation fluid of lucerne herbage. Vet Med 2022; 67(6): 309.
  • Ölmez M, Makav M. The effect of pennyroyal (Mentha pulegium L.) on growth performance and some serum biochemical parameters in New Zealand rabbits. Van Vet J 2021; 32(3): 119-24.
  • Pandur E, Micalizzi G, Mondello L, Horváth A, Sipos K, Horváth G. Antioxidant and anti-inflammatory effects of thyme (Thymus vulgaris L.) essential oils prepared at different plant phenophases on Pseudomonas aeruginosa LPS-activated THP-1 macrophages. Antioxidants 2022; 11(7): 1330.
  • Pastorelli G, Serra V, Vannuccini C, Attard E. Opuntia spp. as alternative fodder for sustainable livestock production. Animals 2022; 12(13): 1597.
  • Terrett OM, Dupree P. Covalent interactions between lignin and hemicelluloses in plant secondary cellwalls. Curr Opin Biotechnol 2019; 56: 97-104.
  • Vakili AR, Khorrami B, Mesgaran MD, Parand E. The effects of thyme and cinnamon essential oils on performance, rumen fermentation and blood metabolites in Holstein calves consuming high concentrate diet. Asian-Australas J Anim Sci 2013; 26(7): 935-44.
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Bilimleri (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Gönül Pirci 0009-0000-0980-5209

Sena Yılmaz Öztaş 0000-0002-0161-4923

Kanber Kara 0000-0001-9867-1344

Kurşat Yılmaz 0000-0001-9131-757X

Gönderilme Tarihi 26 Nisan 2025
Kabul Tarihi 28 Ekim 2025
Yayımlanma Tarihi 26 Aralık 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 22 Sayı: 3

Kaynak Göster

APA Pirci, G., Yılmaz Öztaş, S., Kara, K., Yılmaz, K. (2025). Comparison of in vitro Ruminal Fermentation Variables of Pennyroyal (Mentha pulegium L.) Herbage with Conventional Forages. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 22(3), 145-152. https://doi.org/10.32707/ercivet.1684585
AMA Pirci G, Yılmaz Öztaş S, Kara K, Yılmaz K. Comparison of in vitro Ruminal Fermentation Variables of Pennyroyal (Mentha pulegium L.) Herbage with Conventional Forages. Erciyes Üniv Vet Fak Derg. Aralık 2025;22(3):145-152. doi:10.32707/ercivet.1684585
Chicago Pirci, Gönül, Sena Yılmaz Öztaş, Kanber Kara, ve Kurşat Yılmaz. “Comparison of in vitro Ruminal Fermentation Variables of Pennyroyal (Mentha pulegium L.) Herbage with Conventional Forages”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 22, sy. 3 (Aralık 2025): 145-52. https://doi.org/10.32707/ercivet.1684585.
EndNote Pirci G, Yılmaz Öztaş S, Kara K, Yılmaz K (01 Aralık 2025) Comparison of in vitro Ruminal Fermentation Variables of Pennyroyal (Mentha pulegium L.) Herbage with Conventional Forages. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 22 3 145–152.
IEEE G. Pirci, S. Yılmaz Öztaş, K. Kara, ve K. Yılmaz, “Comparison of in vitro Ruminal Fermentation Variables of Pennyroyal (Mentha pulegium L.) Herbage with Conventional Forages”, Erciyes Üniv Vet Fak Derg, c. 22, sy. 3, ss. 145–152, 2025, doi: 10.32707/ercivet.1684585.
ISNAD Pirci, Gönül vd. “Comparison of in vitro Ruminal Fermentation Variables of Pennyroyal (Mentha pulegium L.) Herbage with Conventional Forages”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 22/3 (Aralık2025), 145-152. https://doi.org/10.32707/ercivet.1684585.
JAMA Pirci G, Yılmaz Öztaş S, Kara K, Yılmaz K. Comparison of in vitro Ruminal Fermentation Variables of Pennyroyal (Mentha pulegium L.) Herbage with Conventional Forages. Erciyes Üniv Vet Fak Derg. 2025;22:145–152.
MLA Pirci, Gönül vd. “Comparison of in vitro Ruminal Fermentation Variables of Pennyroyal (Mentha pulegium L.) Herbage with Conventional Forages”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, c. 22, sy. 3, 2025, ss. 145-52, doi:10.32707/ercivet.1684585.
Vancouver Pirci G, Yılmaz Öztaş S, Kara K, Yılmaz K. Comparison of in vitro Ruminal Fermentation Variables of Pennyroyal (Mentha pulegium L.) Herbage with Conventional Forages. Erciyes Üniv Vet Fak Derg. 2025;22(3):145-52.