Evaluation of Serum Methylglyoxalate, D-lactate and L-lactate Levels in Calves with Acute Neonatal Diarrhea

Öz

Lactate metabolism is critical for the healthy survival of animals. The imbalance of this metabolism leads to mortality. Therefore, the aim of this study was to evaluate the serum D-lactate, L-lactate, and serum methylglyoxalate concentrations in diarrheic calves infected with different infectious agents. Calves were grouped as rotavirus (n=15), coronavirus (n=15), and Cryptosporidium (n=15). The control group (n=15) calves were composed of calves determined to be healthy. While the serum methylglyoxalate and D-lactate levels of the control and diarrheic calves were determined by ELISA, the L-lactate level was determined by a blood gas analyzer. The D-lactate concentration of calves infected with rotavirus (P<0.002), coronavirus (P<0.01), and C. parvum (P<0.001) was significantly higher than that of the control group. The L-lactate concentration of the rotavirus (P<0.07) and coronavirus (P<0.006) infected calves was significantly higher than that of the control group. The White blood cell counts of the rotavirus (P<0.001), coronavirus (P<0.001), and C. parvum (P<0.001) calves were significantly higher than the control group. Unlike these, no statistically significant difference was found between the diarrhea calves in all groups and the control group in terms of serum methylglyoxalate concentration (P>0.499). In conclusion, in this study, it was found that D-lactate and L-lactate concentrations were significantly high in diarrhea caused by different infectious agents, and as a result, these metabolites should be taken into consideration and carefully considered in the evaluation of diarrhea in calves. Future studies are needed to evaluate the effects of D-lactate and L-lactate on mortality in calves with diarrhea of different severities.

Anahtar Kelimeler

• Calf
• diarrhea
• lactate
• methyglyoxalate

Proje Numarası

1919B012326751

Akut Neonatal İshalli Buzağılarda Serum Metilglioksal, D-laktat ve L-laktat Düzeyinin Değerlendirilmesi

Öz

Laktat metabolizması, hayvanların sağlıklı bir şekilde hayatta kalmaları için kritiktir. Bu metabolizmanın dengesizliği ölüme yol açar. Bu nedenle sunulan, bu çalışmanın amacı, farklı enfeksiyöz ajanlarla enfekte olmuş ishalli buzağılarda serum D-laktat, L-laktat ve serum metilgliozolat konsantrasyonlarını değerlendirmekti. Buzağılar rotavirüs (n=15), koronavirüs (n=15) ve Cryptosporidium (n=15) olarak gruplandırıldı. Kontrol grubu (n=15) sağlıklı olduğu belirlenen buzağılardan oluşuyordu. Kontrol ve ishalli buzağıların serum metilglioksalat ve D-laktat seviyeleri ELISA ile belirlenirken, L-laktat seviyesi kan gazı analizörü ile belirlenmiştir. Rotavirüs (P<0.002), koronavirüs (P<0.01) ve C. parvum (P<0.001) ile enfekte olmuş buzağıların D-laktat konsantrasyonu kontrol grubuna göre önemli ölçüde daha yüksekti. Rotavirüs (P<0.07) ve koronavirüs (P<0.006) ile enfekte olmuş buzağılardaki L-laktat konsantrasyonu kontrol grubuna göre önemli ölçüde yüksekti. Rotavirüs (P<0.001), koronavirüs (P<0.001) ve C. parvum (P<0.001) ile enfekte buzağıların beyaz kan hücresi sayıları kontrol grubuna göre anlamlı derecede yüksektir. Bunların aksine, tüm gruplardaki ishalli buzağılar ile kontrol grubu arasında serum metilglioxalat konsantrasyonu açısından istatistiksel olarak anlamlı bir fark bulunmamıştır (P>0.499). Sonuç olarak, bu çalışmada, farklı enfeksiyon etkenleri tarafından meydana gelen ishalde D-laktat ve L-laktat konsantrasyonlarının önemli ölçüde yüksek olduğu bulunmuş ve bu nedenle, bu metabolitlerin buzağılardaki ishalin değerlendirilmesinde dikkate alınması ve dikkatlice değerlendirilmesi gerektiği sonucuna varılmıştır. Gelecekteki çalışmalar, farklı şiddette ishal olan buzağılarda D-laktat ve L-laktatın mortalite üzerindeki etkilerini değerlendirmek için gereklidir.

Anahtar Kelimeler

• Buzağı
• ishal
• laktat
• metilglioksalat

Destekleyen Kurum

This study has been financially supported by TÜBITAK-BİDEB-2209-a (Project Number: 1919B012326751).

Proje Numarası

1919B012326751

Etik Beyan

B.Ü HADYEK Decision No: 01/02, Meeting number: 2024/01

Teşekkür

We thank TÜBITAK-BİDEB 2209-a (Project Number: 1919B012326751) for their contributions to the realization of this study.

Kaynakça

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