Üreme Sezonunda Östrüs Senkronizasyonu Uygulanan Laktasyondaki Kilis Keçilerinde Vitamin A, D3, E ve Selenyum Takviyesinin Üreme Performansı Üzerine Etkileri

Yıl 2025, Cilt: 22 Sayı: 3, 213 - 222, 26.12.2025

Mehmet Dede Tiken , Mustafa Kemal Sarıbay , Gökhan Uyanık

https://doi.org/10.32707/ercivet.1771145

Öz

Bu çalışmada, üreme sezonu boyunca Kilis keçilerinde senkronizasyon protokolüne ek olarak A, D3, E vitaminleri ve selenyum (Se) uygulamasının fertilite parametreleri üzerindeki etkileri araştırılmıştır. Toplam 70 Kilis keçisi rastgele vit-min (n = 35) ve kontrol (n = 35) gruplarına ayrılmıştır. Tüm keçilere 9 gün süreyle 20 mg flugeston asetat içeren vajinal sünger uygulanmıştır. Sünger yerleştirildiği gün, vit-min grubuna intramüsküler olarak 200.000 IU A vitamini, 30.000 IU D3 vitamini, 20 mg E vitamini, 1 mg sodyum selenit ve ilave 60 mg E vitamini uygulanırken, Kontrol grubuna herhangi bir takviye yapılmamıştır. Süngerlerin çıkarıldığı gün, her iki gruba 500 IU eCG ve 250 µg kloprostenol uygulanmış, vit-min grubuna ayrıca A, D3 ve E vitaminlerini içeren ikinci bir enjeksiyon yapılmıştır. Östrüs takibi, süngerlerin çıkarılmasından 24-72 saat sonra tekelerin günde iki kez sürüye katılmasıyla gerçekleştirilmiştir. Gebelik tanısı, aşımdan sonraki 50. günde transabdominal ultrasonografi ile yapılmış, yavru sayısı doğumda belirlenmiştir. Östrüs oranları her iki grupta da %100 olarak tespit edilmiştir (P>0.05). Gebelik oranı vit-min grubunda (%68.57), kontrol grubuna (%48.57) kıyasla sayısal olarak daha yüksek olsa da istatistiksel fark bulunmamıştır (P>0.05). Benzer şekilde, yavru verimi vit-min grubunda daha yüksek olmasına rağmen anlamlı bulunmamıştır (P>0.05). Buna karşın fekondite oranı vit-min grubunda (%154.28), kontrol grubuna (%91.42) göre anlamlı derecede yüksek bulunmuştur (P<0.05). Çoğul gebelik oranları açısından gruplar arasında fark saptanmamıştır (P>0.05). Sonuç olarak, senkronizasyon protokolüne vitamin-mineral takviyesinin eklenmesi, laktasyondaki Kilis keçilerinde fekonditeyi artırarak üreme performansını iyileştirmiştir. Bu yaklaşımın mera bazlı sistemlerde uygulanabilir ve ekonomik açıdan avantajlı olabileceği düşünülmektedir. Ancak, etkisini daha net bir şekilde ortaya koymak için daha büyük örneklem büyüklüklerine sahip kapsamlı çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

Keçi , vitamin , mineral , fertilte parametreleri , üreme mevsimi

Proje Numarası

None

Effects of Vitamin A, D3, E, and Selenium Supplementation on Reproductive Performance in Lactating Kilis Goats Applied with Estrus Synchronization During the Breeding Season

Öz

This study investigated the effects of supplementation with vitamins A, D3, E, and selenium (Se) on fertility parameters in Kilis goats during the breeding season, in addition to a synchronization protocol.
A total of 70 Kilis goats were randomly divided into vit-min (n = 35) and control (n = 35) groups. All goats received a vaginal sponge containing 20 mg of flugestone acetate for 9 days. On the day the sponge was inserted, the vit-min group received intramuscular injections of 200,000 IU of vitamin A, 30,000 IU of vitamin D3, 20 mg of vitamin E, 1 mg of sodium selenate, and an additional 60 mg of vitamin E, while the control group received no supplements. On the day the sponges were removed, both groups received 500 IU of eCG and 250 µg of cloprostenol, and the vit-min group also received a second injection containing vitamins A, D3, and E. Estrus monitoring was performed by introducing bucks to the flock twice daily, 24-72 hours after the removal of sponges. Pregnancy diagnosis was made by transabdominal ultrasonography on the 50th day after mating, and litter size was determined at kidding. Estrus rates were found to be 100% in both groups (P>0.05). Although the pregnancy rate was numerically higher in the vit-min group (68.57%) compared to the control group (48.57%), there was no statistically significant difference (P>0.05). Similarly, although litter size was higher in the vit-min group, it was not statistically significant (P>0.05). In contrast, the fecundity rate was significantly higher in the vit-min group (154.28%) compared to the control group (91.42%) (P<0.05). No difference was found between the groups in terms of multiple pregnancy rates (P>0.05). In conclusion, adding vitamin-mineral supplementation to the synchronization protocol improved reproductive performance in lactating Kilis goats by increasing fecundity. This approach is considered applicable and potentially economically advantageous in pasture-based systems. However, comprehensive studies with larger sample sizes are needed to more clearly demonstrate its effectiveness.

Anahtar Kelimeler

Goat , vitamin , mineral , fertility parameters , breeding season

Etik Beyan

This study was conducted with the approval of the Hatay Mustafa Kemal University Animal Experiments Local Ethics Committee, Hatay, Türkiye (2024/04-06).

Destekleyen Kurum

None

Proje Numarası

None

Teşekkür

This article was produced from the master's thesis of the first author, Mehmet Dede Tiken.

Kaynakça

• Abdelrahman MM, Al-Karablieh EK. Effect of vitamins AD3E injections on reproductive performance and net cash revenue from Awassi sheep raised under semi-intensive system. J King Saud Univer 2002; 14 (1): 15-22.
• Acar BD, Birdan MK, Özenç E, Yeni D, Doğan İ. Effectiveness of different progesterone analogues and GnRH on reproductive parameters in nulliparous Saanen goats at the end of the transition period. Kafkas Üniv Vet Fak Derg 2013; 19(7): 181-6.
• Ahmed OMM, Hassen A, Lehloenya K. The effect of β-carotene supplementation above requirement on the production and reproduction performance of female sheep and goats-a mini review. Aceh J Anim Sci 2024; 9(2): 60-6.
• Amarantidis I, Karagiannidis A, Saratsis PH, Brikas P. Efficiency of methods used for estrous synchronization in indigenous Greek goats. Small Rumin Res 2004; 52(3): 247-52.
• Awawdeh MS, Eljarah AH, Ababneh MM. Multiple injections of vitamin E and selenium improved the reproductive performance of estrus-synchronized Awassi ewes. Trop Anim Health Prod 2019; 51(6): 1421-6.
• Birdane MK, Avdatek F. Effect of vitamin A, D3, E treatment on fertility in the Pırlak sheep. Kocatepe Vet J 2020; 13(2): 179-84.
• Darcan NK, Silanikove N. The advantages of goats for future adaptation to Climate Change: A conceptual overview. Small Rumin Res 2018; 163: 34-8.
• Efe M, Sarıbay MK, Ürer EK, Köse AM. The effect of selenium, vitamin E, vitamin A and vitamin D3 applications on fertility in Awassi sheep with estrus synchronization during the breeding season. Turkish J Agric-Food Sci Tech 2023; 11(8): 1331-6.
• El-Moghazy M, Ashmawy T, Akl F. Effect of supplementation different forms of selenium on reproductive performance of Zaraibi goats. Damietta J Agric Sci 2025; 4(1): 165-74.
• Eşki F, Kurt S, Demir PA. Effect of different estrus synchronization protocols on estrus and pregnancy rates, oxidative stress and some biochemical parameters in Hair goats. Small Rumin Res 2021; 198: 106348.
• Farahavar A, Rostami Z, Alipour D, Ahmadi A. The effect of pre-breeding vitamin E and selenium injection on reproductive performance, antioxidant status, and progesterone concentration in estrus-synchronized Mehraban ewes. Trop Anim Health Prod 2020; 52(4): 1779-86.
• Fernando PDSM, Ko DO, Piao MJ, Kang KA, Herath HMUL, Hyun JW. Protective effect of luteolin against oxidative stress‑mediated cell injury via enhancing antioxidant systems. Mol Med Rep 2024; 30(1): 1-10.
• Giorgio D, Di Trana A, Di Gregorio P, Rando A, Avondo M, Bonanno A, Di Grigoli, A. Oxidative status of goats with different CSN1S1 genotypes fed ad libitum with fresh and dry forages. Antioxidants 2020; 9(3): 224.
• Habeeb HMH, Kutzler MA. Estrus synchronization in the sheep and goat. Veterinary Clinics: Food Anim Prac 2021; 37(1): 125-37.
• Hashemi M, Safdarian M. Efficiency of different methods of estrus synchronization followed by fixed time artificial insemination in Persian downy does. Anim Reprod 2018; 14(2): 413-7.
• Holtz W, Sohnrey B, Gerland M, Driancourt MA. Ovsynch synchronization and fixed-time insemination in goats. Theriogenology 2008; 69(7): 785-92.
• Kaya S, Koçak G. Üreme mevsimindeki Romanov koyunlarında senkronizasyon protokolleriyle birlikte D vitamini enjeksiyonunun bazı üreme parametreleri üzerine etkisi. Van Vet J 2022; 33(3): 76-9.
• Kaya S, Koçak G, Demir MC, Kaçar C. The effect of vitamin e-trace mineral treatments on reproductive performance in Morkaraman sheep during the breeding season. Kafkas Üniv Vet Fak Derg 2023; 29(2): 161-9.
• Keskin M, Gül S, Biçer O, Mutlukoca G. Importance of Kilis Goat Breeding in Turkey. 11. International Animal Science Conference. October, 431-6, 2019; Nevşehir-Turkey.
• Kılboz Eİ, Karaca F. Üreme mevsimi dışında genç keçilerde flugeston asetat vaginal sünger ve norgestomet kulak implantı uygulamalarıyla östrüslerin uyarılması. Van Vet J 2010; 21(1): 1-6.
• Kuru M, Ögün M, Oral H, Kükürt A, Makav M, Kulaksız R. The use of controlled internal drug release for synchronization augmented oxidative and nitrosative stress and leptin levels in Georgian goats. J Cell Neurosci Oxid Stress 2016; 8(1): 541-2.
• Lohani M, Bhandari D. The importance of goats in the world. Prof Agric Workers J 2021; 6(2): 9-21.
• Martinez-Ros P, Lozano M, Hernandez F, Tirado A, Rios-Abellan A, López-Mendoza MC, Gonzalez-Bulnes A. Intravaginal device-type and treatment-length for ovine estrus synchronization modify vaginal mucus and microbiota and affect fertility. Animals 2018; 8(12): 226.
• Miyamoto K, Sato EF, Kasahara E, Jikumaru M, Hiramoto K, Tabata H, Inoue M. Effect of oxidative stress during repeated ovulation on the structure and functions of the ovary, oocytes, and their mitochondria. Free Radic Biol Med 2010; 49(4): 674-81.
• Musa SI, Bitto II, Ayoade JA, Oyedipe OE. Effects of vitamin E and selenium on fertility and lamb performance of Yankasa sheep. Open J Vet Med 2018; 8(09): 167.
• Özar E, Sarıbay MK, Köse AM, Sertkol R. Effects of selenium, vitamin E, and β-carotene administration on fertility of Awassi ewes synchronized for estrus in non-breeding season. MAE Vet Fak Derg 2022; 7(3): 167-74.
• Pasquariello R, Anipchenko P, Pennarossa G, Crociati M, Zerani M, Brevini TA, Maranesi M. Carotenoids in female and male reproduction. Phytochemistry 2022; 204: 113459.
• Pu Y, Wang Z, Bian Y, Zhang F, Yang P, Li Y, Zhang X. All‐trans retinoic acid improves goat oocyte nuclear maturation and reduces apoptotic cumulus cells during in vitro maturation. Anim Sci J 2014; 85(9): 833-9.
• Ronda-Borzone P, Donoso G, Riveros FJL. Food security and livelihood challenges of extensive goat production systems in desertification areas. CABI Reviews 2022; 17(44): 1-3.
• Semerci A, Çelik AD. Türkiye’de küçükbaş hayvan yetiştiriciliğinin genel durumu. MKÜ Ziraat Fak Derg 2016; 21(2): 182-96.
• Sen U, Onder H. The effect of estrus synchronization programmes on parturition time and some reproductive characteristics of Saanen goats. J Appl Anim Res 2016; 44(1): 376-9.
• Shahrokhi SZ, Ghaffari F, Kazerouni F. Role of vitamin D in female reproduction. Clin Chim Acta 2016; 455: 33-8.
• Sönmez M, Bozkurt T, Türk G, Gür S, Kızıl M, Yüce A. The effect of vitamin E treatment during preovulatory period on reproductive performance of goats following estrous synchronization using intravaginal sponges. Anim Reprod Sci 2009; 114(1-3): 183-92.
• Soto-Heras S, Paramio MT Impact of oxidative stress on oocyte competence for in vitro embryo production programs. Res Vet Sci 2020; 132: 342-50.
• Stenhouse C, Suva LJ, Gaddy D, Wu G, Bazer FW. Phosphate, calcium, and vitamin D: key regulators of fetal and placental development in mammals. Adv Exp Med Biol 2022; 1354: 77-107.
• Swelum AA, Abdelnour SA, Sheiha AM, Hashem NM, Taha AE, Khafaga AF, Abd El-Hack ME. Enhancing in vitro oocyte maturation competence and embryo development in farm animals: roles of vitamin-based antioxidants–a review. Ann Anim Sci 2022; 22(1): 3-19.
• Syaiful FL, Jaswandi J, Mundana M, Yendraliza Y, Udin Z. Estrus response and pregnancy rate of swamp buffalo: The use of multivitamins in different estrus synchronization hormone protocols. J Adv Vet Anim Res 2024; 11(3): 729.
• TÜİK. (2024). Hayvansal Üretim İstatistikleri. https://data.tuik.gov.tr/Bulten/Index?p=Hayvansal-Uretim-Istatistikleri-2024-53935; Accessed Date: 23.08.2025.