Morin, Oksidatif Stres ve İnflamasyonu Azaltarak Kadmiyumun Neden Olduğu Akut Renal Toksisiteye Karşı Koruma Sağlar

Öz

Kadmiyum (Cd), başta böbrek dokusu olmak üzere çeşitli organlarda birikim gösteren, yüksek derecede toksik bir ağır metaldir. Kalıcılığı ve biyobirikim özelliği nedeniyle çevresel ve mesleki açıdan önemli bir halk sağlığı sorunu oluşturmaktadır. Doğal olarak pek çok meyve ve tıbbi bitkide bulunan bir flavonoid olan Morin, güçlü antioksidan ve antiinflamatuvar özellikleri ile bilinmektedir. Ancak Morin’in Cd’nin neden olduğu nefrotoksisiteye karşı potansiyel koruyucu etkisi tam olarak aydınlatılmamıştır. Bu çalışmanın amacı, Morin’in Cd kaynaklı oksidatif stres ve inflamasyona karşı böbrek dokusundaki olası koruyucu etkilerini değerlendirmek ve bu etkilerin olası moleküler mekanizmalarını incelemektir. Çalışmada, 50 adet erişkin erkek Sprague-Dawley sıçanı beş gruba ayrılmıştır: Kontrol, Cd (6.5 mg/kg, i.p.), Morin100+Cd, Morin200+Cd ve Morin200. Kadmiyum beş gün boyunca intraperitoneal olarak uygulanırken, Morin enjeksiyondan bir saat önce intragastrik yolla verilmiştir. Kadmiyum uygulaması, böbrek dokusunda MDA ve proinflamatuvar sitokin (TNF-α, IL-1β, IL-6, IFN-γ) düzeylerini anlamlı şekilde artırırken, antioksidan enzim aktiviteleri (SOD, CAT, GPx, GSH) ve antiinflamatuvar sitokinler (IL-10, IL-4) azalmıştır. Özellikle 200 mg/kg Morin tedavisi bu değişiklikleri tersine çevirerek oksidatif stresi azaltmış, antioksidan ve sitokin dengesini yeniden düzenlemiştir. Sonuç olarak, Morin’in Cd’nin neden olduğu böbrek toksisitesine karşı oksidatif stres ve inflamasyonu baskılayarak güçlü bir nefroprotektif etki gösterdiği ve bu etkinin oksidatif stres ve inflamatuvar yanıtın baskılanması ile ilişkili olabileceği düşünülmektedir. Morin, ağır metal kaynaklı böbrek hasarlarının önlenmesinde potansiyel bir doğal terapötik ajan olarak değerlendirilebilir. 

Anahtar Kelimeler

• İnflamasyon
• kadmiyum
• morin
• nefrotoksisite
• oxidative stress

Etik Beyan

Atatürk Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu'nun 2025/10 sayılı kararı

Morin Protects Against Cadmium-Induced Acute Renal Toxicity by Reducing Oxidative Stress and Inflammation

Öz

Cadmium (Cd) is a highly toxic heavy metal that accumulates primarily in the kidneys, where it induces oxidative stress, inflammation, and cellular damage. Cd exposure is a major environmental and occupational concern due to its persistence and bioaccumulative nature. Morin, a natural flavonoid abundant in various fruits and medicinal plants, possesses potent antioxidant and anti-inflammatory properties. However, its potential protective efficacy against Cd-induced nephrotoxicity has not been fully elucidated. This study aimed to evaluate the protective effects of Morin against Cd-induced oxidative and inflammatory damage in rat kidney tissue and to assess its association with oxidative stress and inflammatory responses. Fifty adult male Sprague-Dawley rats were randomly divided into five groups: Control, Cd (6.5 mg/kg, i.p.), Morin100+Cd (100 mg/kg Morin + Cd), Morin200+Cd (200 mg/kg Morin + Cd), and Morin200 (200 mg/kg Morin alone). Cadmium was administered intraperitoneally for 5 consecutive days, while Morin was given intragastrically one hour before Cd injection. Cadmium exposure significantly elevated malondialdehyde (MDA) levels and proinflammatory cytokines (TNF-α, IL-1β, IL-6, IFN-γ) while reducing the activities of antioxidant enzymes (SOD, CAT, GPx, GSH) and anti-inflammatory cytokines (IL-10, IL-4). Morin treatment, particularly at 200 mg/kg, markedly ameliorated these alterations by suppressing oxidative stress, restoring antioxidant enzyme activities, and modulating cytokine balance. These findings indicate that Morin exerts a strong nephroprotective effect against Cd-induced renal toxicity through attenuation of oxidative stress and inflammation, possibly associated with its antioxidant and anti-inflammatory properties. Therefore, Morin may represent a promising natural therapeutic candidate for the prevention of heavy metal-induced renal injury. 

Anahtar Kelimeler

• Cadmium
• inflammation
• morin
• nephrotoxicity
• oxidative stress

Etik Beyan

Atatürk University Animal Experiments Local Ethics Committee under decision number 2025/10

Kaynakça

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