Türkiye’de Yetiştirilen Anadolu Mandalarında Butirofilin (BTN1A1) Gen Polimorfizminin HaeIII Restriksiyon Enzimi ile Araştırılması

Öz

Butirofilin süt yağının salgılanmasında önemli rolü olan bir transmembran glikoproteinidir. Bu araştırmada, Türkiye’de yetiştirilen Anadolu mandalarında butirofilin (BTN1A1) geninin genotip ve allel frekanslarının belirlenmesi amaçlanmıştır. Bu çalışmada toplam olarak 120 manda polimeraz zincir reaksiyonu-restriksiyon parçacık uzunluk polimorfizmi (PCR-RFLP) metodu ile genotiplendirilmiştir. Çalışmada yapılan PCR işlemi sonunda elde edilen 501 bç’lik amplikonlar elde edilmiştir. Elde edilen PCR ürünlerinin HaeIII restiriksiyon enzimi ile kesilmeleri sonucunda incelenen örneklerde sadece A alleli ve AA genotipinin bulunduğu görülmüştür. Bu çalışma Anadolu mandalarında BTN1A1 gen polimorfizminin araştırıldığı ilk çalışmadır. Çalışma sonunda, Anadolu mandalarında BTN1A1 geninde polimorfizm bulunamamıştır. 

Anahtar Kelimeler

• Anadolu mandası,butirofilin geni,genetik polimorfizm,RFLP

Investigation of Butyrophilin (BTN1A1) Gene Polymorphism by Using HaeIII Restriction Enzyme in Anatolian Buffaloes Reared in Turkey

Öz

Butyrophilin is a transmembrane glycoprotein and plays a critical role in secretion of milk lipid. The aim of this study was to identify genotype and allele frequencies of butyrophilin (BTN1A1) gene in Anatolian buffaloes in Turkey. A total of 120 buffalos were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in this study. As a result of PCR process, 501 bp amplicons were obtained from the Anatolian buffaloes examined in this study. After digestion of the PCR products with HaeIII restriction enzyme, only A allele and AA genotype were found. This study provided first information on the polymorphism on BTN1A1 gene in Anatolian buffaloes. In conclusion, there was no polymorphism found in the BTN1A1 gene in Anatolian buffaloes. 

Anahtar Kelimeler

• Anatolian buffalo,butyrophilin gene,genetic polymorphism,RFLP

Kaynakça

1. 1. Ashwell MS, Ogg SL, Mather IH. The bovine butyrophilin gene maps to chromosome 23. Anim Genet 1996; 27(3): 171-3.
2. 2. Atasever S, Erdem H. Manda yetiştiriciliği ve Türkiye’deki geleceği. OMÜ Zir Fak Derg 2008; 23(1): 59-64.
3. 3. Bhattacharya TK, Misra SS, Sheikh FD, Dayal S, Vohra V, Kumar P, Sharma A. Variability of milk fat globule membrane protein gene between cattle and riverine buffalo. DNA Seq 2004; 15(5-6): 326-31.
4. 4. Bhattacharya TK, Misra SS, Sheikh FD, Sukla S, Kumar P, Sharma A. Effect of butyrophilin gene polymorphism on milk quality traits in crossbred cattle. Asian-Aust J Anim Sci 2006; 19(7): 922-6.
5. 5. Bhattacharya TK, Sheikh FD, Sukla S, Kumar P, Sharma A. Differences of ovine butyrophilin gene (exon 8) from its bovine and bubaline counterpart. Small Rum Res 2007; 69(1-3): 198-202.
6. 6. El Nahas SM, Hondt HA, Womack JE. Current status of the river buffalo (Bubalus bubalis L) gene map. J Hered 2001; 92(3): 221-5.
7. 7. Ertuğrul M, Akman N, Dellal G, Goncagül T. Hayvan gen kaynaklarının korunması ve Türkiye hayvan gen kaynakları. Beşinci Türkiye Ziraat Mühendisliği Teknik Kongresi. Ocak, 17-21, 2000; Ankara-Türkiye.
8. 8. Food and Agricultural Organization of the United Nations (FAO). http://faostat3.fao.org/browse/Q/QA/E; Erişim tarihi: 02.10.2015.

9. 9. Franke WW, Heid HW, Grund C, Winter S, Freundenstein C, Schmid E, Jarasch ED, Keenan TW. Antibodies to the major insoluble milk fat globule membrane associated protein: specific location in apical regions of lactating epithelial cells. J Cell Biology 1981; 89(3): 485-94.
10. 10. Husaini Y, Wilkins RJ, Davey HW. Identification of five point mutations, including an AluI RFLP, in the bovine butyrophilin gene. Anim Genet 1999; 30(5): 400-1.
11. 21. Rychtářová J, Sztankóová Z, Kyselová J, Zink V, Štípková M, Vacek M, Štolc L. Effect of DGAT1, BTN1A1, OLR1, and STAT1 genes on milk production and reproduction traits in the Czech Fleckvieh breed. Czech J Anim Sci 2014; 59(2): 45-53.
12. 22. Sadr AS, Nasiri MTB, Alami-Saeid Kh, Fayazi J, Roshanfekr H, Mohammadi M. DNA polymorphism of butyrophilin gene by PCR-RFLP technique. African J Biotechnol 2008; 7(14): 2527-9.
13. 23. Soysal İ, Kök S, Gürcan EK. Mandalarda alyuvar potasyum polimorfizmi üzerine bir araştırma. Tekirdağ Ziraat Fak Derg 2005; 2(2): 189-93.
14. 24. Taylor C, Everest M, Smith C. Restriction fragment length polymorphism in amplification products of the bovine butyrophilin gene: assignment of bovine butyrophilin to bovine chromosome 23. Anim Genet 1996; 27(3): 183-5.
15. 25. Türkiye İstatistik Kurumu (TÜİK). Hayvancılık İstatistikleri. https://biruni.tuik.gov.tr/hayvancilikapp/hayvancilik.zul. Erişim tarihi: 09.02.2016.
16. 26. Yardibi H, Gürsel FE, Ates A, Akıs I, Hosturk GT, Oztabak K. BTN1A1, FABP3 and TG genes polymorphism in East Anatolian red cattle breed and South Anatolian red cattle breed. Afr J Biotechnol 2013; 12(20): 2802-7.
17. 27. Werf V JHJ. Marker Assisted Selection in Sheep and Goats. In: "Marker Assisted Selection (MAS) in Crops, Livestock, Forestry and Fish: Current Status and the Way Forward", FAO Invited Book, 2007; Chapter 13, p. 231.
18. 28. Zegeye A, Ashwel M, Ogg S, Rexroad C, Maher IH. RFLP markers in the bovine butyrophilin gene. Anim Genet 1999; 30(5): 385-6.