Yağlı Diyetle Beslenen Farelerde Magnezyumun Leptin ve Trigliserid Düzeylerine Etkisi

Öz

Vücudun birçok biyokimyasal fonksiyonunda rol oynayan magnezyumun (Mg), leptin ve trigliserid düzeyleri üzerine etkisinin araştırılması amaçlanan bu çalışmada materyal olarak iki aylık, 39 adet erkek Swiss albino cinsi fare kullanıldı. Vücut ağırlıkları tartılarak dört gruba ayrıldı. Grup I (Kontrol) standart pellet ve içme suyu, Grup II %31.5 yağ içeren pelet yem ve içme suyu, Grup III %31.5 yağ içeren pelet yem ve 7.5g/L magnezyum sülfat (MgSO₄) içeren su, Grup IV standart pelet yem ve 7.5g/L MgSO₄ içeren su ile on iki hafta boyunca beslendi. Daha sonra anestezi işlemine geçilerek kalpten kan örnekleri alındı ve santrifüj edilerek serumları ayrıldı. Ötenazi işleminden sonra abdominal bölgeden alınan yağ doku örnekleri homojenize edildi. Elde edilen süpernatantlarda leptin, kan serumlarında Mg ve trigliserid analizi yapıldı. Uygulama sonunda Grup I, II ve III’ün ilk ve son ağırlıkları arasındaki fark istatistiksel olarak anlamlı (p<0.01) bulundu. Grup II’nin leptin düzeyi Grup I’e göre anlamlı derecede yüksek (p<0.001). Grup IV’ün leptin düzeyinde Grup III’e göre önemli azalma (p<0.001) saptandı. Grup II’nin Mg düzeylerinde Grup I’e, Grup III’ün Mg düzeyleride Grup IV’e göre istatistiksel olarak önemli azalma (p<0.05) belirlendi. Grup II’nin trigliserid düzeyleri Grup I’e göre, Grup III’ün trigliserid düzeyleri ise Grup IV’e göre istatistiksel olarak anlamlı artış (p<0.001) görüldü. Sonuç olarak, yapılan araştırmada yağlı diyetin leptin ve trigliserid düzeylerinde artışa ve Mg düzeylerinde azalmaya neden olduğu saptandı. Yağlı diyete bağlı olarak artan leptin ve trigliserid düzeylerinin normal seviyelere düşürülmesi bakımından Mg uygulanmasının alternatif bir yöntem olarak kullanılabileceği kanaatine varıldı.

Anahtar Kelimeler

• Leptin,magnezyum,obezite,trigliserid,yağlı diyet

The Effect of Magnesium on Leptin and Triglyceride Levels in Mice Fed on a Fatty Diet

Öz

This study was aimed to investigate the effects of magnesium (Mg), playing a role in many biochemical functions of the body, on leptin and triglyceride levels, and 39 two month-old, male Swiss albino mice were used as materials. They were divided into four groups by weighing their bodies. Group I (Control) was fed on standard pellet food and drinking water, Group II was fed on the diet containing 31.5% oil and drinking water, Group III was fed on the diet containing 31.5% oil and drinking water containing 7.5g/L magnesium sulphate (MgSO₄) and Group IV was fed on standard pellet food and drinking water containing 7.5g/L MgSO₄ for twelve weeks. Then blood samples were taken from the heart by passing anesthesia and their serum was separated by centrifuging. Fat tissue samples taken from abdominal region were homogenized after the euthanasia. Leptin was analyzed in the obtained supernatants. Mg and triglyceride were analyzed in the blood serum. At the end of the treatment, the difference between the initial and the final weight of Group I, II and III were found to be statistically significant (p<0.01). In this present study, leptin level of Group II was detected significantly high (p<0.001) when compared to that of Group I. Compared to Group III, it was determined that there was a significant decrease (p<0.001) in the leptin level of Group IV. Compared to the Group I, it was determined that there was a significant decrease (p<0.05) in the Mg level of Group II, and compared to Group IV, it was determined that there was a significant decrease (p<0.05) in the Mg level of Group III. Compared to the Group I, it was determined that there was a significant increase (p<0.001) in the triglyceride level of Group II. Compared to Group IV, it was determined that there was a significant increase (p<0.001) in the triglyceride level of Group III. The results indicated that a fatty diet led to increase in the levels of leptin and triglyceride and decrease in the Mg levels. Depending on a fatty diet, it was concluded that Mg implementation could be used as an alternative method in terms of increasing leptin and triglyceride levels to reach back to normal levels.

Anahtar Kelimeler

• Fatty diet,leptin,magnesium,obesity,triglyceride

Kaynakça

1. 1. Ahren B, Mansson S, Gıngerıch RL, Havel PJ. Regulation of plasma leptin in mice: influence of age, high-fat diet, and fasting. Am J Physiol Regul Integr Comp Physiol 1997; 273(2): 113-20.
2. 2. Ainslie DA, Proietto J, Fam BC, Thorburn AW. Short-term, high-fat diets lower circulating leptin concentrations in rats. Am J Clin Nutr 2000; 71(2): 438-42.
3. 3. Amin KA, Nagy MA. Effect of carnitine and herbal mixture extract on obesity induced by high fat diet in rats. Diabetol Met Synd 2009; 1(1): 17-31.
4. 4. Aslan K, Serdar Z, Tokullugil HA. Multifonksiyonel Hormon: Leptin. Uludağ Üniv Tıp Fak Derg 2004; 30(2): 113-8.
5. 5. Barbagallo M, Dominguez LJ. Magnesium metabolism in type 2 diabetes mellitus, metabolic syndrome and insulin resistance. Arc Biochem Biophy 2007; 458(1): 40-7.
6. 6. Brabant G, Horn R, Mayr M, Wurster U, Schnabel D, Heidenreich F. Free and protein bound leptin are distinct and independently controlled factors in energy regulation. Diabetol 2000; 43(4): 438-42.
7. 7. Buettner R, Scholmerich J, Bollheimer LC. High-fat diets: Modeling the metabolic disorders of human obesity in rodents. Obesity 2007; 15(4): 798-808.
8. 8. Cha MC, Chou CJ, Boozer CN. High-fat diet feeding reduces the diurnal variation of plasma leptin concentration in rats. Metabolism 2000; 49(4): 503-7.

9. 9. Chaudhary DP, Sharma R, Bansal DD. Implications of magnesium deficiency in type 2 diabetes: A review. Biol Trace Element Res 2010; 134(2): 119-29.
10. 10. Chen H, Li-Jun L, Jian-Jun Z, Boa X, Rui L. Effect of soybean oligosaccharides on blood lipid, glucose levels and antioxidant enzymes activity in high fat rats. Food Chem 2010; 119(4): 1633-6.
11. 21. He K, Liu K, Daviglus ML, Morris SJ, Loria CM, Horn VL, Jacobs DR, Savage PJ. Magnesium intake and incidence of metabolic syndrome among young adults. Circulation 2006; 113(13): 1675-82.
12. 22. Huerta MG, Roemmich JN, Kington ML, Bovbjerg VE, Weltman AL. Magnesium deficiency is associated with insulin resistance in obese children. Diabetes Care 2005; 28(5): 1175-81.
13. 23. İşbilen B, Arı Z, Var A, Onur E, Uyanık BS. Yüksek yağ içeren diyet ile beslenen ratlarda DHEAS’ın leptin, lipid profili ve endotel fonksiyonu üzerine etkileri. Fırat Üniv Sağ Bil Derg 2007; 21(3): 109-16.
14. 24. Jang EH, Park CS, Lee SK, Pie JE, Kang JH. Excessive nitric oxide attenuates leptin-mediated signal transducer and activator of transcription 3 activation. Life Sci 2007; 80(7): 609-17.
15. 25. Jose B, Jain V, Vikram NK, Agarwala A, Saini S. Serum magnesium in overweight children. Indian Pediatr 2011; 49(2): 109-12.
16. 26. Kalaivanisailaja J, Manju V, Nalini N. Lipid profile in mice fed a high-fat diet after exogenous leptin administration. Polish J Pharmacol 2003; 55(5): 763-9.
17. 27. Kim SO, Yun SJ, Jung B, Lee EH, Hahm DH, Shim I, Lee HJ. Hypolipidemic effects of crude extract of adlay seed (Coix lachrymajobi var. mayuen) in obesity rat fed high fat diet: Relations of TNF-a and leptin mRNA expressions and serum lipid levels. Life Sci 2004; 75(11): 1391-404.
18. 28. Lee JS, Lee MK, Ha TY, Bok SH, Park HM, Jeong KS, Woo MN, Do M, Yeo JY, Choi MS. Supplementation of whole persimmon leaf improves lipid profiles and suppresses body weight gain in rats fed high-fat diet. Food Chem Toxicol 2006; 44(11): 1875-83.
19. 29. Saris NE, Mervaala E, Karppanen H, Khawaja JA, Lewenstam A. Magnesium: An update on physiological, clinical and analytical aspects. Clin Chim Acta 2000; 294(1-2): 1-26.
20. 30. Sinha MK, Opentanova I, Ohannesian JP, Kolaczynski JW, Heiman ML, Hale J. Evidence of free and bound leptin in human circulation. J Clin Invest 1996; 98(6): 1277-82.
21. 41. Zhang Y, Proenca R, Maffel M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature 1994; 372(6505): 425-32.