Araştırma Makalesi
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Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi

Yıl 2018, , 238 - 246, 04.12.2018
https://doi.org/10.32707/ercivet.477313

Öz

Transforme edici büyüme faktörü-beta1 (TGF-β1), ovaryum fonksiyonlarının düzenlenmesinde önemli rol oyna-maktadır. Sunulan çalışmada, sıçan ovaryumlarında düşük doz capsaicin (CAP) uygulamasının, follikülogenezis ve atrezi sürecinde TGF-β1 ekspresyonuna etkisinin olup/olmadığı incelendi. Otuz adet 21 günlük dişi Sprague-Dawley sıçan üç eşit grubu ayrıldı. İlk gruba hiçbir enjeksiyon yapılmadı (kontrol A grubu), ikinci gruba (deney grubu) taşıyıcı solüsyon içinde eritilen CAP (0.5 mg/kg/gün) enjeksiyonu yapıldı, üçüncü gruba (kontrol B grubu) sadece taşıyıcı solüs-yon enjeksiyonu yapıldı. Onbeş günlük uygulama periyodundan sonra hayvanlar eter inhalasyonu ile uyutularak ovar-yumları alındı ve rutin histolojik prosedür uygulandı. TGF-β1, işaretli streptavidin-biotin tekniği kullanılarak gösterildi. Deney grubunda oositler, tüm folliküllerin (primordiyal, primer, sekonder, preantral, antral folliküller) granuloza hücrele-ri, teka hücreleri ve intersitisyel hücreler pozitif boyandı. Kontrol grubundaki boyanmada genel olarak deney grubuna benzerdi, fakat preantral ve antral folliküllerin granuloza hücrelerinde boyanma yoktu. Deney grubundaki TGF-β1 bo-yanması, kontrol gruplarından daha yoğundu. Deney ve kontrol gruplarında atretik folliküllerde TGF-β1 ekspresyonu gözlenmedi. Sonuç olarak, CAP, follikülogenezis sırasında ovaryan hücrelerdeki kendi reseptörüne bağlanarak TGF-β1 ekspresyonunu arttırmıştır.

Kaynakça

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Toplam 1 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Makalesi
Yazarlar

Berrin Zık

Yayımlanma Tarihi 4 Aralık 2018
Gönderilme Tarihi 14 Kasım 2017
Kabul Tarihi 17 Nisan 2018
Yayımlandığı Sayı Yıl 2018

Kaynak Göster

APA Zık, B. (2018). Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 15(3), 238-246. https://doi.org/10.32707/ercivet.477313
AMA Zık B. Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi. Erciyes Üniv Vet Fak Derg. Aralık 2018;15(3):238-246. doi:10.32707/ercivet.477313
Chicago Zık, Berrin. “Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 15, sy. 3 (Aralık 2018): 238-46. https://doi.org/10.32707/ercivet.477313.
EndNote Zık B (01 Aralık 2018) Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 15 3 238–246.
IEEE B. Zık, “Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi”, Erciyes Üniv Vet Fak Derg, c. 15, sy. 3, ss. 238–246, 2018, doi: 10.32707/ercivet.477313.
ISNAD Zık, Berrin. “Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 15/3 (Aralık 2018), 238-246. https://doi.org/10.32707/ercivet.477313.
JAMA Zık B. Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi. Erciyes Üniv Vet Fak Derg. 2018;15:238–246.
MLA Zık, Berrin. “Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, c. 15, sy. 3, 2018, ss. 238-46, doi:10.32707/ercivet.477313.
Vancouver Zık B. Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi. Erciyes Üniv Vet Fak Derg. 2018;15(3):238-46.