The Effects of Ionizing Radiation on the Hepatocyte Morphology and Proliferating Cell Nuclear Antigen (PCNA) Expression in Albino Mice

Yıl 2008, Cilt: 5 Sayı: 2, 61 - 67, 01.06.2008

S. Karahan K. Çavuşoğlu H. T. Atmaca E. Yalçın

https://izlik.org/JA47AX56HX

Öz

-

Anahtar Kelimeler

Fare , hepatosit , immunohistokimya , iyonlaştırıcı radyasyon , PCNA

Albino Farelerde İyonlaştırıcı Radyasyonun Hepatosit Morfolojisi ve Prolifere Hücre Çekirdek Antijeni (PCNA) Ekspresyonu Üzerindeki Etkileri

https://izlik.org/JA47AX56HX

Öz

amaçlandı. Normal ve iyonlaştırıcı radyasyona maruz kalan albino fare hepatositlerindeki prolifere hücre çekirdek antijeni (PCNA) ekspresyonu immünoperoksidaz boyama tekniğiyle belirlendi. Radyasyon grubundaki fareler, çalışmanın bir ve yedinci günlerinde 30 dakika süre ile Kobalt 60-gama (1,333 MeV) ışını ile 10 Gy dozunda radyasyona maruz bırakıldı ve onbeşinci gün hayvanların nekropsileri yapıldı. Kontrol grubu fare hepatositlerinde PCNA immünopozitifliği sadece hücre çekirdeğinde gözlenirken, radyasyon grubu albino farelerde hem çekirdek hem de sitoplazmada tespit edildi. Kontrol grubunda, PCNA immünopozitif hepatositlerin oranı (%27,76±20,4), radyasyon grubuna oranla (%7,53±5,43) daha yüksekti (p

Anahtar Kelimeler

Fare , hepatosit , immunohistokimya , iyonlaştırıcı radyasyon , PCNA

Kaynakça

• Albert MD, Bucher NL, 1960. Latent injury and repair in rat liver induced to regenerate at intervals after X-radiation. Cancer Res, 20: 1514-1522.
• Balajee AS, Dianova I, Bohr VA, 1999. Oxidative damage-induced PCNA complex formation pigmentosum group A but reduced in Cockayne syndrome group B cells. Nucleic Acids Res, 27 (22): 4476-4482. xeroderma
• Blankenship TN, King BF, 1994. Developmental expression of Ki-67 antigen and proliferating cell nuclear antigen in macaque placentas. Dev Dyn, 201(4) : 324- 33.
• Bravo R, Frank R, Blundell Pa, Macdonald- Bravo H, 1987. Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta. Nature, 326 (6112): 515-517.
• Casasco A, Giordano M, Danova M, Casasco M, Icaro Cornaglia A, Calligaro A, 1993. PC10 monoclonal antibody to proliferating cell nuclear antigen as probe for cycling cell detection in developing tissues. A combined immunocytochemical and flow cytometric study. Histochemistry, 99 (3): 191-199.
• Connolly KM, Bogdanffy MS, 1993. Evaluation of proliferating cell nuclear antigen (PCNA) as an endogenous marker of cell proliferation in rat liver: a dual-stain comparison with 5- bromo-2'-deoxyuridine. Cytochem, 41(1):1-6. J Histochem
• Eldrige SR, Butterworth BE, Goldsworthy TL, 1993. Proliferating cell nuclear antigen: a mar- ker for hepatocellular proliferation in rodents. Environ Health Perspect, 101 Suppl 5: 211- 218.
• Fujji E, KarasawaY, KumanoE, SakuraiT, MisawaY, Mori T, Ito T, Suzuki M, Sugimoto T, 2004. Nuclearity and BrdU Labeling of Rat Hepatocytes in Cytocentrifuge Preparations of Freshly Isolated Hepatocytes with Cumulative Labeling of Bromodeoxyuridine. J Toxicol Pathol, 17: 43-49.
• Gandillet A, Alexandre E, Holl V, Royer C, Bischoff P, Cinqualbre J, Wolf P, Jaeck D, Richert L, 2003. Hepatocyte ploidy in normal young rat. Comp Biochem Physiol A Mol Integr Physiol, 134(3): 665-673.
• Gorla Gr, Malhi H, Gupta S, 2001. Polyploidy associated with oxidative injury attenuates proliferative potential of cells. J Cell Sci, 114: 2943-2951.
• Guidotti JE, Brégerie O, Robert A, Debey P, Brechot C, Desdouets C, 2003. Liver cell polyploidization: a pivotal role for binuclear hepatocytes. J Biol Chem, 278(21): 19095- 19101.
• Guryev DV, 2005. Histologic assesment of regenerating rat liver under low-dose radiation exposure. International Congress Series, 1276: 181-181.
• Hall PA, Levison DA, Woods AL, Yu CC, Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejohn R, Dover R, Waseem NH, Lane DP, 1990. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol, 162: 285-294.
• Jeong WI, Do SH, Kim TH, Jeong DH, Hong IH, Ki MR, Kwak DM, Lee SS, Jee YH, Kim SB, Jeong KS, 2007. Acute effects of fast neutron irradiation on mouse liver. J Radiat Res, 48 (3): 233-240.
• Kamel OW, Warnke RA, Banks PM, 1992. Localization of proliferating cell nuclear antigen (PCNA/Cyclin) in workshop cases of Hodgkin's lymphoma. Semin Diagn Pathol, 9(4): 311- 314. and non-Hodgkin's
• Kelman Z, Hurwitz J, 1998. Protein-PCNA interactions: a DNA-scanning mechanism? Trends Biochem Sci, 23 (7): 236-238.
• Komatsu K, Wharton W, Hang H, Wu C, Singh S, Lieberman HB, Pledger WJ, Wang HG, 2000. PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition. Oncogene, 19 (46): 5291-5297.
• Kurki P, Vanderlaan M, Dolbeare F, Gray J, Tan EM, 1986. Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle. Exp Cell Res, 166 (1): 209-219.
• Maharwal J, Samarth RM, Saini MR, 2005. Antioxidative effect of Rajgira leaf extract in liver of Swiss albino mice after exposure to different doses of gamma radiation. Phytother Res, 19 (8): 717-720.
• Melchiorri C, Chieco P, Zedda AI, Coni P, Ledda-Columbano GM, Columbano A, 1993. Ploidy and nuclearity of rat hepatocytes after compensatory regeneration or mitogen- induced liver growth. Carcinogenesis, 14 (4):1825-1830.
• Sharma R, Sharma J, 2005. Modification of gamma ray induced changes in the mouse hepatocytes by Centella asiatica extract: in vivo studies. Phytother Res, 19 (7): 605-611.
• Sigal SH, Rajvanshi P, Gorla GR, Sokhi RP, Saxena R, Gebhard Dr JR, Reid LM, Gupta S, 1999. Partial hepatectomy-induced polyploidy attenuates hepatocyte replication and