Conjunctiva-Associated Lymphoid Tissue in Angora Rabbit

Öz

-

Anahtar Kelimeler

• Ankara tavşanı, CALT, FAE, HEV

Ankara Tavşanında Konjunktiva ile İlişkili Lenfoid Doku

Öz

Bu çalışmada, Ankara tavşanında konjunktiva ile ilişkili lenfoid dokunun (Conjunctiva-associated lymphoid tissue,CALT) yapısı ışık ve elektron mikroskobik olarak incelendi. Araştırmada özel yetiştiricilerden sağlanan 10 adet sağlıklı,erişkin Ankara tavşanı kullanıldı. Asetik asit ile yapılan incelemede konjunktivanın lenfoid ve non-lenfoid bölgeler içerdiği gözlendi. Agregat lenf folliküllerinin nazolakrimal punktum çevresinde kümelendiği belirlendi. Histolojik olarak agregatlenf foliküllerinin epitele yakınlaştığı ve folikül ile ilişkili epiteli (Follicle-associated epithelium, FAE) oluşturduğu görüldü.Bu foliküller germinal merkez, korona, subepiteliyal dom bölgesi ve interfoliküler alanlardan oluşmaktaydı. Dom bölgesini örten FAE, kadeh hücresi içermeyen tek katlı yassı epitelden meydana gelirken; lenfoid yapı içermeyen bölge yapısında kadeh hücreleri bulunan yalancı çok katlı pirizmatik epitel ile örtülüydü. İnterfoliküler alanlarda ve folliküllerin dipkısımlarında yüksek endoteliyal venüllere rastlandı. İnce yapı düzeyinde, FAE’de elektron yoğun, ince ve dar bir sitoplazmaya sahip olan membranöz hücreler (M hücreleri) görüldü. Bu hücreler apikal membranında kısa ve düzensizmikrofoldlar, sitoplazmasında vezikül ve vakuoller ile karakterizeydi. M hücrelerinin bazolateralinde ise lenfositler vemakrofajlar görüldü. Sonuç olarak, Ankara tavşanında CALT’ın, mukoza ile ilişkili lenfoid dokuların karakteristik özelliklerine sahip olduğu belirlendi

Anahtar Kelimeler

• Ankara tavşanı, CALT, FAE, HEV

Kaynakça

1. Astley RA, Kennedy RC, Chodosh J, 2003. Structural and conjunctival lymphoid follicles in the baboon (Papio anubis). Exp Eye Res, 76: 685-694. of
2. Astley RA, Chodosh J, 2005. Selective uptake of iron oxide by rabbit conjunctival lymphoid follicles. Cornea, 24: 334-336.
3. Aştı RN, Kurtdede N, Altunay H, Özen A, 2000. Electron conjunctiva (CALT) in Angora goats. Wochenschr, 107: 196-198. studies on associated lymphoid tissue Dtsch Tierarztl
4. Aştı RN, Kurtdede N, Altunay H, Özen A, 2000. Ankara keçilerinin konjunktiva ile ilişkili lenfoid mikroskobik çalışmalar. Ankara Üniv Vet Fak Derg, 47: 31-37. üzerinde ışık
5. Bayraktaroğlu AG, Aştı RN, 2009. Light and electron microscopic studies on conjunctiva associated lymphoid tissue (CALT) in cattle. Revue Med Vet, 160(5): 252-257.
6. Beyaz F, 2004. M hücreleri: membranöz epitel hücreleri. Erciyes Üniv Vet Fak Derg, 1: 133- 138.
7. Cain C, Phillips TE, 2008. Developmental changes in conjunctiva-associated lymphoid tissue of the rabbit. Invest Ophthalmol Vis Sci, 49: 644-649.
8. Chodosh J, Nordquist RE, Kennedy RC, 1998. Anatomy lymphoepithelium. Adv Exp Med Biol, 438: 557-565. conjunctival

9. Chodosh J, Nordquist RE, Kennedy RC, 1998. anatomy Comparative conjunctival lymphoid tissue: a putative mucosal immune site. Dev Comp Immunol, 22, 621-630. of mammalian
10. Crossmon G, 1937. A modification of Mallory’s connective tissue stain with a discussion of the principles involved. Anat Rec, 69: 33-88.
11. Elson CO, 1997. Advances in mucosal immunity. Drugs Suppl, 1: 13-14.
12. Fix AS, Arp LH, 1989. Conjunctiva-associated lymphoid tissue in normal and Bordetella avium-infected turkeys. Vet Pathol, 26: 222- 230. 13. Fix AS, characterization lymphoid tissue in chickens. Am J Vet Res, 52: 1852-1859. 1991. Morphologic
13. conjunctiva-associated 14. Franklin RM, Remus LE, 1984. tissue:
14. Conjunctival-associated
15. evidence a role in the secretory immune
16. system. Invest Ophthalmol Vis Sci, 25: 181- 187. lymphoid
17. Gebert A, Rothkotter HJ, Pabst R, 1996. M cells in Peyer's patches of the intestine. Int Rev Cytol, 167, 91-159.
18. Guiliano EA, Moore CP, Phillips TE, 2002. Morphological evidence of M cells in healthy canine tissue. Greafes Arch Clin Exp Ophthalmol, 240: 220-226. lymphoid
19. Hannant D, 2002. Mucosal immunology: overview and potential in the Veterinary species. Vet Immunol Immunopathol, 87: 265- 267. 18. Karnovsky MJ, 1965. Formaldehyde- glutaraldehyde fixative of high osmolalitiy for use in electron microscopy. J Cell Biol, 27: 137-138.
20. Kelsall BL, Leon F, Smythies LE, Smith PD, 2004. Antigen handling and presentation by mucosal dendritic cells and macrophages. Mestecky Bienenstock J. Mcghee JR. MayeIn L. eds. Mucosal Immunology. London: J Academic Press, pp.105. ME. Strober W.
21. Knop E, Knop N, 2003. Eye-associated lymphoid tissue (EALT) is continuously spread throughout the ocular surface from the lacrimal gland to the lacrimal drainage system. Opthalmologe, 11: 929- 942.
22. Knop N, Knop E, 2000. Conjunctiva- associated lymphoid tissue in the human eye. Invest Ophthalmol Vis Sci, 41: 1270- 1279.
23. Knop N, Knop E, 2005. Ultrastructural anatomy of CALT follicles in the rabbit reveals characteristics of M-cells, germinal centres and high endothelial venules. J Anat, 207: 409-426.
24. Latkovic S, 1989. Ultrastructure of M cells in the conjunctival epithelium of the guinea pig. Curr Eye Res, 8: 751-755.
25. Liebler-Tenorio E, Pabst R, 2006. MALT structure and function in farm animals. Vet Res, 37: 257-280.
26. Neutra MR, Pringault E, Kraehenbuhl JP, 1996. Antigen sampling across epithelial barriers and induction of mucosal immune responses. Annu Rev Immunol, 14: 275-300.
27. Neutra MR, Kraehenbuhl JP, 2004. Cellular and molecular basis for antigen transport across epithelial barriers. Mestecky J. Lamm ME. Strober W. Bienenstock J. Mcghee JR. MayeIn L. eds. Mucosal Immunology. London: Academic Press, pp.155.
28. Ruskell GL, 1995. Organization and cytology of lymphoid in the cynomolgus monkey conjunctiva. Anat Rec, 243: 153-164.
29. Russell MW, Martin MH, Wu H, Hollingshead SK, Moldoveanu Z, Mestecky J, 2000. Strategies immunization againts mucosal infections. Vaccines, 19: 122-127.
30. Sakimoto T, Shoji J, Inada N, Saito K, Iwasaki Y, Sawa M, 2002. Histological study of conjunctiva-associated lymphoid tissue in mouse. Japanese J Ophthalmol, 46: 364-369.
31. Sandıkçı M, Eren Ü, Kum S, 2005. Alpha- naphthyl acetate esterase activity in the spleen, associated lymphoid tissues of camels (Camelus dro-medarius). Revue Med Vet, 156: 99-103. and conjunctiva
32. Veneable JH, Coggeshall R, 1965. A simplified lead citrate stain for use in electron microscopy. J Cell Biol, 25: 407-408.
33. Wotherspoon AC, Hardman-Lea S, Isaacson PG, 1994. Mucosa-associated lymphoid tissue (MALT) in the human conjunctiva. J Pathol, 174: 33-37.
34. Astley RA, Kennedy RC, Chodosh J, 2003. Structural and conjunctival lymphoid follicles in the baboon (Papio anubis). Exp Eye Res, 76: 685-694. of
35. Astley RA, Chodosh J, 2005. Selective uptake of iron oxide by rabbit conjunctival lymphoid follicles. Cornea, 24: 334-336.
36. Aştı RN, Kurtdede N, Altunay H, Özen A, 2000. Electron conjunctiva (CALT) in Angora goats. Wochenschr, 107: 196-198. studies on associated lymphoid tissue Dtsch Tierarztl
37. Aştı RN, Kurtdede N, Altunay H, Özen A, 2000. Ankara keçilerinin konjunktiva ile ilişkili lenfoid mikroskobik çalışmalar. Ankara Üniv Vet Fak Derg, 47: 31-37. üzerinde ışık
38. Bayraktaroğlu AG, Aştı RN, 2009. Light and electron microscopic studies on conjunctiva associated lymphoid tissue (CALT) in cattle. Revue Med Vet, 160(5): 252-257.
39. Beyaz F, 2004. M hücreleri: membranöz epitel hücreleri. Erciyes Üniv Vet Fak Derg, 1: 133- 138.
40. Cain C, Phillips TE, 2008. Developmental changes in conjunctiva-associated lymphoid tissue of the rabbit. Invest Ophthalmol Vis Sci, 49: 644-649.
41. Chodosh J, Nordquist RE, Kennedy RC, 1998. Anatomy lymphoepithelium. Adv Exp Med Biol, 438: 557-565. conjunctival
42. Chodosh J, Nordquist RE, Kennedy RC, 1998. anatomy Comparative conjunctival lymphoid tissue: a putative mucosal immune site. Dev Comp Immunol, 22, 621-630. of mammalian
43. Crossmon G, 1937. A modification of Mallory’s connective tissue stain with a discussion of the principles involved. Anat Rec, 69: 33-88.
44. Elson CO, 1997. Advances in mucosal immunity. Drugs Suppl, 1: 13-14.
45. Fix AS, Arp LH, 1989. Conjunctiva-associated lymphoid tissue in normal and Bordetella avium-infected turkeys. Vet Pathol, 26: 222- 230. 13. Fix AS, characterization lymphoid tissue in chickens. Am J Vet Res, 52: 1852-1859. 1991. Morphologic
46. conjunctiva-associated 14. Franklin RM, Remus LE, 1984. tissue:
47. Conjunctival-associated
48. evidence a role in the secretory immune
49. system. Invest Ophthalmol Vis Sci, 25: 181- 187. lymphoid
50. Gebert A, Rothkotter HJ, Pabst R, 1996. M cells in Peyer's patches of the intestine. Int Rev Cytol, 167, 91-159.
51. Guiliano EA, Moore CP, Phillips TE, 2002. Morphological evidence of M cells in healthy canine tissue. Greafes Arch Clin Exp Ophthalmol, 240: 220-226. lymphoid
52. Hannant D, 2002. Mucosal immunology: overview and potential in the Veterinary species. Vet Immunol Immunopathol, 87: 265- 267. 18. Karnovsky MJ, 1965. Formaldehyde- glutaraldehyde fixative of high osmolalitiy for use in electron microscopy. J Cell Biol, 27: 137-138.
53. Kelsall BL, Leon F, Smythies LE, Smith PD, 2004. Antigen handling and presentation by mucosal dendritic cells and macrophages. Mestecky Bienenstock J. Mcghee JR. MayeIn L. eds. Mucosal Immunology. London: J Academic Press, pp.105. ME. Strober W.
54. Knop E, Knop N, 2003. Eye-associated lymphoid tissue (EALT) is continuously spread throughout the ocular surface from the lacrimal gland to the lacrimal drainage system. Opthalmologe, 11: 929- 942.
55. Knop N, Knop E, 2000. Conjunctiva- associated lymphoid tissue in the human eye. Invest Ophthalmol Vis Sci, 41: 1270- 1279.
56. Knop N, Knop E, 2005. Ultrastructural anatomy of CALT follicles in the rabbit reveals characteristics of M-cells, germinal centres and high endothelial venules. J Anat, 207: 409-426.
57. Latkovic S, 1989. Ultrastructure of M cells in the conjunctival epithelium of the guinea pig. Curr Eye Res, 8: 751-755.
58. Liebler-Tenorio E, Pabst R, 2006. MALT structure and function in farm animals. Vet Res, 37: 257-280.
59. Neutra MR, Pringault E, Kraehenbuhl JP, 1996. Antigen sampling across epithelial barriers and induction of mucosal immune responses. Annu Rev Immunol, 14: 275-300.
60. Neutra MR, Kraehenbuhl JP, 2004. Cellular and molecular basis for antigen transport across epithelial barriers. Mestecky J. Lamm ME. Strober W. Bienenstock J. Mcghee JR. MayeIn L. eds. Mucosal Immunology. London: Academic Press, pp.155.
61. Ruskell GL, 1995. Organization and cytology of lymphoid in the cynomolgus monkey conjunctiva. Anat Rec, 243: 153-164.
62. Russell MW, Martin MH, Wu H, Hollingshead SK, Moldoveanu Z, Mestecky J, 2000. Strategies immunization againts mucosal infections. Vaccines, 19: 122-127.
63. Sakimoto T, Shoji J, Inada N, Saito K, Iwasaki Y, Sawa M, 2002. Histological study of conjunctiva-associated lymphoid tissue in mouse. Japanese J Ophthalmol, 46: 364-369.
64. Sandıkçı M, Eren Ü, Kum S, 2005. Alpha- naphthyl acetate esterase activity in the spleen, associated lymphoid tissues of camels (Camelus dro-medarius). Revue Med Vet, 156: 99-103. and conjunctiva
65. Veneable JH, Coggeshall R, 1965. A simplified lead citrate stain for use in electron microscopy. J Cell Biol, 25: 407-408.
66. Wotherspoon AC, Hardman-Lea S, Isaacson PG, 1994. Mucosa-associated lymphoid tissue (MALT) in the human conjunctiva. J Pathol, 174: 33-37.