Kısa Süreli Saklanan Tavşan Spermasının Spermatolojik Parametreleri Üzerine Taxifolinin Etkileri

Yıl 2020, Cilt: 17 Sayı: 2, 164 - 172, 27.07.2020

Selcan Sevim Serpil Sarıözkan

https://doi.org/10.32707/ercivet.774342

Cited By: 1

Öz

Bu çalışmanın amacı, tavşan spermasının kısa süreli saklanması süresince (0., 6., 12., ve 24. saat) taxifolinin spermatolojik parametreler üzerine etkisini araştırmaktır. Araştırmada sperma vericisi olarak 8 adet ergin Yeni Zelanda tavşanı kullanıldı. Sperma suni vajen yardımıyla haftada üç kez alındı. Tavşanlardan alınan ejakulatlar 37oC’de değerlendirildikten sonra karıştırıldı. Sperma örnekleri 4 ayrı gruba ayrıldı. Daha sonra sperma numuneleri Tris (K-Tris), Tris+10 µl taxifolin (10 TT), Tris+100 µl taxifolin (100 TT) ve Tris+500 µl taxifolin (500 TT) ile sulandırıldı. Sulandırılmış sperma numuneleri 4oC’ de 24 saat süreyle kısa süreli saklandı. Saklama süresinin ardından bazı spermatolojik parametreler (motilite, akrozom anomali oranı, toplam anormal spermatozoa oranı ve HOST) yönünden analiz edildi. Deneysel çalışmalar 10 kez tekrar edildi. Çalışmanın sonucunda, tüm deneysel gruplarda 24. saat sonunda anormal spermatozoa oranında artış ve plazma membran bütünlüğü ile motilite oranında düşme saptandı. Kontrol grubu verileriyle karşılaştırıldığında 100 TT grubu 6. (%82.50), 12. (%80) ve 24. (%77.50) saatlerde istatistiki olarak en yüksek motilite oranını verdi (P<0.001). Kısa süreli saklamanın 24. saatinde 100 µl taxifolin ilavesinin diğer gruplara kıyasla akrozomal morfolojik yapının bütünlüğünü istatistiki açıdan önemli derecede koruduğu ve akrozom anomalisi oranı (%7) ile toplam spermatozoa anomali oranını (%8.50) düşürdüğü ve plazma membran bütünlüğünü de istatistiki olarak önemli derecede koruduğu saptandı (P<0.001). Sonuç olarak, 100 µl taxifolin ilavesinin tavşan spermasının kısa süreli saklanmasında etkin koruyucu bir rol oynadığı tespit edildi.

Anahtar Kelimeler

Kısa süreli saklama, spermatolojik parametreler, antioksidan, tavşan, taxifolin

The Effects of Taxifolin on Spermatological Parameters of Liquid Stored Rabbit Semen

Öz

The aim of this study was to investigate the effect of taxifolin on spermatological parameters of rabbit semen during liquid storage (0, 6, 12, and 24th h). In this study, eight adult New Zealand male rabbits were used as semen donors. Semen was taken with the aid of artificial vagina three times a week. Ejaculates collected from bucks were evaluated and pooled at 37oC. Semen samples were divided into 4 groups then were diluted with Tris (K-Tris), Tris + 10 µl taxifolin (10 TT), Tris + 100 µl taxifolin (100 TT) and Tris + 500 µl taxifolin (500 TT). The diluted semen samples were liquid stored at 4°C up to 24th h. After the storage period, some spermatological parameters (the percentage of motility, acrosome - total spermatozoa abnormality and HOST) were analyzed. Experimental studies were replicated 10 times. As a result of the study, the percentage of abnormal spermatozoa increased and plasma membrane integrity and motility rate decreased at the end of 24th h in all experimental groups. When compared to the control group data, 100 µl taxifolin addition gave the highest motility rate at 6th (82.50%), 12th (80%) and 24th (77.50%) hour (P <0.001). At the 24th hour of liquid storage, 100 µl taxifolin addition was found to protect statistically significantly the integrity of acrosomal structure and plasma membrane compared to other groups. In addition, it was determined that the percentage of acrosome (7%) and total spermatozoa abnormality (8.50%) was low in the 100 TT group when compared to other groups (P <0.001). In conclusion, it was found that addition of 100 µl taxifolin plays effective protective role on short-term storage of rabbit semen.

Anahtar Kelimeler

Antioxidant, liquid storage, rabbit, spermatological parameters, taxifolin

Kaynakça

• Agarwal A, Durairajanayagam D, Halabi J, Peng J, Vazquez-Levin M. Proteomics, oxidative stress and male infertility. Reprod Bio Med Online 2014; 29(1): 32-58.
• Agarwal A, Prabakaran SA, Said TM. Prevention of oxidative stress injury to sperm. J Androl 2005; 26(6): 654-60.
• Aitken RJ, Baker MA. Oxidative stress and male reproductive biology. Reprod Fert Dev 2004; 16(5): 581-8.
• Avdatek F, Yeni D, Gündoğan M. Merinos koçlarda spermaya katılan antioksidanların kısa süreli saklama sırasında spermatolojik parametreler ve DNA hasarı üzerine etkileri. Kocatepe Vet J 2018; 11(2): 126-33.
• Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol Reprod Dev 2000; 55(3): 282-8.
• Bucak MN, Tekin N, Kulaksız R. Koç spermasının kısa süreli saklanmasında antioksidanların etkisi. Lalahan Hay Araşt Enst Derg 2007; 47(2): 15-21.
• Castellini C, Cardinali R, Dal Bosco A, Minelli A, Camici O. Lipid composition of the main fractions of rabbit semen. Theriogenology 2006; 65(4): 703-12.
• Cotelle N. Role of flavonoids in oxidative stress. Curr Top Med Chem 2001; 1(6): 569-90.
• De Lamirande E, Jiang H, Zini A, Kodama H, Gagnon C. Reactive oxygen species and sperm physiology. Rev Reprod 1997; 2(1): 48-54.
• Garrido N, Meseguer M, Simon C, Pellicer A, Remohi J. Prooxidative and anti-oxidative imbalance in human semen and its relation with male fertility. Asian J Androl 2004; 6(1): 59-66.
• Gupta MB, Bhalla TN, Gupta CR, Mitra GP, Bhargava KP. Anti-inflammatory activity of taxifolin. Japan J Pharmacol 1971; 21(3): 377-82.
• Hafez ESE. Reproduction and Breeding Techniques for Laboratory Animals. Philadelphia: Lea and Febiger, 1970; pp. 273-98.
• Holt WV. Basic aspects of frozen storage of semen. Anim Reprod Sci 2000; 62(1-3): 3-22.
• Kim S, Lee YJ, Kim YJ. Changes in sperm membrane and ROS following cryopreservation of liquid boar semen stored at 15oC. Anim Reprod Sci 2011; 124(1-2): 118-24.
• Kuang H, Tang Z, Zhang C, Wang Z, Li W, Yang C, Wang Q, Yang B, Kong AN. Taxifolin activates the Nrf2 anti-oxidative stress pathway in mouse skin epidermal JB6 P+cells through epigenetic modifications. Int J Mol Sci 2017; 18(7): 1546.
• Morris GJ, Acton E, Avery S. A novel approach to sperm cryopreservation. Hum Reprod 1999; 14(4): 1013-21.
• Paulenz H, Söderquist L, Perez-Pe R, Berg KA. Effect of different extenders and storage temperatures on sperm viability of liquid ram semen. Theriogenology 2002; 57(2): 823-36.
• Revell SG, Mrode RA. An osmotic resistance test for bovine semen. Anim Reprod Sci, 1994; 36: 77-86.
• Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biol Med 1996; 20(7): 933-56.
• Roca J, Martinez S, Vázquez JM, Lucas X, Parrilla I, Martinez EA. Viability and fertility of rabbit spermatozoa diluted in Tris-buffer extenders and stored at 15oC. Anim Reprod Sci 2000; 64(1-2): 103-12.
• Rosato MP, Iaffaldano N. Effect of chilling temperature on the long-term survival of rabbit spermatozoa held either in a Tris-based or a jellified extender. Reprod Domest Anim 2011; 46(2): 301-8.
• Sabir SA, El-Gindy YM, Morshedy SA, Zahran SM, Ahmed MH, Zeweil HS. Semen quality, sex hormone and antioxidant status of male rabbits as influenced by two forms of onion. Egypt Poult Sci 2019; (31-39): 31-8.
• Saleh RA, Agarwal A. Oxidative stress and male infertility: from research bench to clinical practise. J Androl 2002; 23(6): 737-52.
• Sarıözkan S, Bucak MN, Tuncer PB, Ulutaş Alkım P, Bilgen A. The influence of cysteine and taurine on microscopic-oxidative stres parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology 2009; 58(2): 134-8.
• Sarıözkan S, Özdamar S, Türk G, Cantürk F, Yay A. In vitro effects of L-carnitine and glutamine on motility, acrosomal abnormality, and plasma membrane integrity of rabbit sperm during liquid-storage. Cryobiology 2014; 68(3): 349-53.
• Sarıözkan S, Türk G, Cantürk F, Yay A, Eken A, Akçay A. The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology 2013; 67(1): 1-6.
• Schafer S, Holzman A. The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci 2000; 59(3-4): 201-11.
• Sunil C, Xu B. An insight into the health-promoting effects of taxifolin (dihydroquercetin). Phytochemistry 2019; 166: 112066.
• Tittarelli C, Savignone CA, Arnaudín E, Stornelli MC, Stornelli MA, De La Sota RL. Effect of storage media and storage time on survival of spermatozoa recovered from canine and feline epididymides. Theriogenology 2006; 66 (6-7): 1637-40.
• Topal F, Nar M, Gocer H, Kalin P, Kocyigit UM, Gülçin İ, Alwasel SH. Antioxidant activity of taxifolin: an activity-structure relationship. J Enzyme Inhib Med Chem 2016; 31(4): 674-83.
• Zhang HQ, Wang YJ, Yang GT, Gao QL, Tang MX. Taxifolin inhibits receptor activator of NF-kappaB ligand-induced osteoclastogenesis of human bone marrow-derived macrophages in vitro and prevents lipopolysaccharide-induced bone loss in vivo. Pharmacology 2019; 103(1-2): 101-9.
• Zhu Z, Li R, Lv Y, Zeng W. Melatonin protects rabbit spermatozoa from cryo-damage via decreasing oxidative stress. Cryobiology 2019; 88: 1-8.