The Effects of Ionizing Radiation on the Hepatocyte Morphology and Proliferating Cell Nuclear Antigen (PCNA) Expression in Albino Mice

Yıl 2008, Cilt: 5 Sayı: 2, 61 - 67, 01.06.2008

S. Karahan K. Çavuşoğlu H. T. Atmaca E. Yalçın

Öz

-

Anahtar Kelimeler

Fare, hepatosit, immunohistokimya, iyonlaştırıcı radyasyon, PCNA

Albino Farelerde İyonlaştırıcı Radyasyonun Hepatosit Morfolojisi ve Prolifere Hücre Çekirdek Antijeni (PCNA) Ekspresyonu Üzerindeki Etkileri

Öz

amaçlandı. Normal ve iyonlaştırıcı radyasyona maruz kalan albino fare hepatositlerindeki prolifere hücre çekirdek antijeni (PCNA) ekspresyonu immünoperoksidaz boyama tekniğiyle belirlendi. Radyasyon grubundaki fareler, çalışmanın bir ve yedinci günlerinde 30 dakika süre ile Kobalt 60-gama (1,333 MeV) ışını ile 10 Gy dozunda radyasyona maruz bırakıldı ve onbeşinci gün hayvanların nekropsileri yapıldı. Kontrol grubu fare hepatositlerinde PCNA immünopozitifliği sadece hücre çekirdeğinde gözlenirken, radyasyon grubu albino farelerde hem çekirdek hem de sitoplazmada tespit edildi. Kontrol grubunda, PCNA immünopozitif hepatositlerin oranı (%27,76±20,4), radyasyon grubuna oranla (%7,53±5,43) daha yüksekti (p

Anahtar Kelimeler

Fare, hepatosit, immunohistokimya, iyonlaştırıcı radyasyon, PCNA

Kaynakça

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