Ratlarda Potasyum Dikromat ile Bor’un Oksidatif Stres ve DNA Hasarı Üzerine Etkileri

Öz

Bu çalışmada, sıçanlarda potasyum dikromat (K2Cr2O7) ve borun (B, borik asit olarak) serum ve karaciğer oksidatif stres ve DNA hasarı üzerine etkileri araştırıldı. Altmış dişi Sprague-Dawley sıçan, her birinde 10 hayvan bulunan altı gruba ayrıldı. Birinci grup kontrol grubu olarak tutuldu. İkinci ve üçüncü gruplara sırasıyla 5 ve 10 mg/kg B; dördüncü gruba 10 mg/kg K2Cr2O7; beş ve altıncı gruplara ise 5 ve 10 mg/kg B ile birlikte K2Cr2O7 2 hafta boyunca oral olarak verildi. Kontrol grupları ile karşılaştırıldığında K2Cr2O7 grubunda serum MDA düzeyleri artarken (P<0.01), TAC düzeyleri azaldı (P<0.001). Serum MDA düzeyleri K2Cr2O7+5 ve 10 mg/kg B gruplarında azaldı; ancak, anlamlı azalma K2Cr2O7+10 mg/kg B grubunda (P<0.01) tespit edildi. Serum TAC düzeyleri K2Cr2O7+B gruplarında sayısal artış göster-di. Karaciğer MDA düzeyi, K2Cr2O7+10 mg/kg B grubunda diğer gruplara göre anlamlı düzeyde azaldı (P<0.01). Grup-lar arasında plazma 8-OHdG düzeyleri açısından fark belirlenemedi. Karaciğer B ve Cr düzeyleri arasında pozitif korelasyon saptandı (P<0.05). Bu çalışmada 10 mg/kg K2Cr2O7 uygulanan ratlarda serum MDA ve TAC düzeyleri olumsuz etkilenirken, K2Cr2O7 grubuna 10 mg/kg B verilmesi, serum ve karaciğer lipid peroksidasyon göstergeleri üzerine olum-lu etki yaptığı söylenebilir.

Anahtar Kelimeler

• Bor
• DNA hasarı
• oksidatif stres
• potasyum dikromat
• rat

Effects of Potassium Dichromate and Boron on Oxidative Stress and DNA Damage in Rats

Öz

In this study, the effects of potassium dichromate (K2Cr2O7) and boron (B, as boric acid) on oxidative stress and DNA damage in rat serum and liver were investigated. Sixty female Sprague-Dawley rats were divided into six groups of 10 animals each. The first group was kept as the control group. The second and third groups received 5 and 10 mg/kg B, respectively, the forth group received 10 mg/kg K2Cr2O7, and the fifth and sixth groups received 5 and 10 mg/kg B plus K2Cr2O7, respectively, orally for two weeks. Compared with the control groups, serum MDA levels increased (P<0.01) and TAC levels decreased (P<0.001) in the K2Cr2O7 group. Serum MDA levels decreased in the K2Cr2O7+5 and 10 mg/kg B groups, but a significant decrease was found in the K2Cr2O7+10 mg/kg B group (P<0.01). Serum TAC levels showed a numerical increase in K2Cr2O7+B groups. The liver MDA level was significantly decreased in the K2Cr2O7+10 mg/kg B group compared to the other groups (P<0.01). There was no difference in plasma 8-OHdG levels between the groups. A positive correlation was observed between liver B and Cr levels (P<0.05). In this study, serum MDA and TAC levels were negatively affected in rats administered 10 mg/kg K2Cr2O7. In contrast, administration of 10 mg/kg B to the K2Cr2O7 group had positively effected on serum and liver lipid peroxidation indicators.

Anahtar Kelimeler

• Boron
• DNA damage
• oxidative stress
• potassium dichromate
• rat

Kaynakça

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