Determination of the Fatty Acid Composition in Muscle Tissue of Squalius cephalus (L.1758) Living in Serban Dam Lake (Afyonkarahisar)

Yıl 2014, Cilt: 30 Sayı: 2, - , 01.04.2014

Sait Bulut Ramazan Mert

Öz

In this study, the fatty acid composition and ?3/?6 fatty acid ratio was determined in muscle samples from 20 chub (Squalius cephalus L., 1758) in Serban Dam Lake on February 2010. The fatty acid composition was analyzed by GC (HP Agilent 7890 A) with FID detector, capillary column (DB-23) and supelco (37 mix) standard. Palmitic acid (PA,%19,374±0,631) and stearic acid (SA,%5,760±0,476) were the major SFA. Oleic acid (OA,%16,825±0710) and palmitoleic acid (PA, %13,601±0,371) were the major MUFA. Docosaheksaenoic acid (DHA, %10,449±1,562), arachidonic acid (AA, %7,338±0,558), eicosapentaenoic acid (EPA, %5,954±0,916), linoleic acid (LA, %4,558±0,440) and linolenic acid (LNA, %3,061±0,449) were the most abundant PUFA. The percentages of total SFA, total MUFA and total PUFA were found %28,414; %32,524; %38,197 of total lipid, respectively. These results have shown that there are significant statistical differences between fatty acid groups (P<0.05). The ?3/?6 ratio in muscle tissues was found to be 1,285. S. cephalus is a significant dietary source of ?3 PUFA (especially DHA, EPA ve AA) for local people and ?3/?6 ratio observed in the recommended values (1-4) in Serban Dam Lake.

Anahtar Kelimeler

Squalius cephalus, fatty acid composition, ω3/ω6 ratio, Serban Dam Lake, Afyonkarahisar

Serban Baraj Gölü’nde (Afyonkarahisar) Yaşayan Squalius cephalus (L., 1758)’un Kas Dokusundaki Yağ Asidi Kompozisyonun Belirlenmesi

Öz

Bu çalışmada Şubat 2010 tarihinde Serban Baraj Gölü’ndeki 20 kefal (Squalius cephalus L., 1758) kas örneğinin yağ asidi bileşimi ?3/?6 oranı belirlenmiştir. Yağ asidi bileşimi FID dedektör, kapiller kolon (DB-23), supelco (37 mix) standartve Gaz Kromatografisi (HP Agilent 7890 A) kullanılarak analiz edilmiştir. Palmitik asit (PA,%19,374±0,631) ve stearik asit (SA,%5,760±0,476) en büyük orandaki SFA’lardır. Oleik asit (OA,%16,825±0710) ve palmitoleik asit (PA, %13,601±0,371) en büyük orandaki MUFA’lardır. Dokosaheksaenoik asit (DHA, %10,449±1,562), araşidonik asit (AA, %7,338±0,558), eikosapentaenoik asit (EPA, %5,954±0,916), linoleik asit (LA, %4,558±0,440) and linolenik asit (LNA, %3,061±0,449) en bol bulunan PUFA’lardır. Toplam SFA, toplam MUFA ve toplam PUFA’nın toplam lipitteki oranları sırasıyla %28,414; %32,524; %38,197 olarak bulunmuştur. Bu sonuçlar yağ asidi gruplarının oranları arasındaki farkın istatistikî olarak önemli olduğunu göstermektedir(P

Anahtar Kelimeler

Squalius cephalus, Yağ asidi kompozisyonu, ω3/ω6 oranı, Serban Baraj Gölü, Afyonkarahisar

Kaynakça

• Innis, S.M., Essential fatty acids in growth and development, Progress in Lipid Research, 30, 39–103, 1991.
• Simopoulos, A. P., Omega-3 fatty acids in health and disease and in growth and development, American Journal of Clinical Nutrition, 54,438–463, 1991.
• Bezard, J., Blond, J.P., Bernard, A., Clouet, P., The metabolism and availability of essential fatty acids in animal and human tissues, Reproduction Nutrition Development, 34, 539– 568, 1994.
• Alasalvar, C., Taylor, K. D. A., Zubcov, E., Shahidi, F., Alexis, M., Differentiation of cultured and wild sea bass (Dicentrarchus labrax): total lipid content, fatty acid and trace mineral composition, Food Chemistry, 79(2), 145–150, 2002.
• Guil-Guerrero, J.L., Venegas, E.V., Cervera, M.A.R., Suarez, M.D., Fatty acid profiles of livers from selected marine fish species, Journal of Food Composition and Analysis, 24, 217–222, 2011.
• Jabeen, F., Chaudhry, A.S., Chemical compositions and fatty acid profiles of three freshwater fish species, Food Chemistry, 125, 991–996, 2011.
• Leaf, A., Weber, P.C., Cardiovascular effects of n-3 fatty acids, New England J Med Food, 318, 549-557, 1988.
• Kinsella, J.E., Lokesh, B., Stone, R.A., Dietary n-3 polyunsaturated fatty acids and amelioration of cardiovascular disease: Possible mechanism, American Journal of Clinical Nutrition, 52, 1–28, 1990.
• Stone, M.D., Fish Consumption, Fish Oil, Lipids, and Coronary Heart Disease, Circulation, 94, 2337-2340, 1996.
• Lovegrove, J.A., Brooks, C.N., Murphy, M.C., Gould, B.J., Williams, C.M., Use of manufactured foods enriched with fish oils as means of increasing long-chain n-3 polyunsaturated fatty acid intake. British Journal Nutrition 67, 223–236, 1997.
• Lemaitre, R.N., King, I.B., Mozaffarian, D., Kuller, L.H., Tracy, R.P., Siscovick, D.S. n3 polyunsaturated fatty acids, fatal ischemic heart disease, and nonfatal myocardial infarction in older adults: The Cardiovascular Health Study, American Journal of Clinical Nutrition, 77(2), 319–325, 2003.
• Ahlgren, G., Blomqvist, P., Boberg, M., Gustafsson, I.B. Fatty acid content of the dorsal muscle an indicator of fat quality in freshwater fish, Journal of Fish Biology, 45, 131–157, 1994.
• Henderson, R.J., Tocher, D.R., The lipid composition and biochemistry of freshwater fish. Prog Lipid Res 26: 281-347, 1987.
• Rahman, S.A., Huah, T.S., Hassan, O., et al. Fatty-acid composition of some Malaysian fresh-water fish, Food chem, 54, 45-49, 1995.
• Luzia, L. A., Sampaio, G. R., Castellucci, C. M. N., Torres, E. A. F. S. The influence of season on the lipid profiles of five commercially important species of Brazilian fish, Food Chemistry, 83(1), 93–97, 2003.