Karga (Corvus corone) özofagus, proventrikulus ve gizzard mukozalarındaki glikokonjugatların lektin histokimyası ile belirlenmesi

Yıl 2012, Cilt: 28 Sayı: 1, 53 - 59, 01.02.2012

Kenan Çınar Emel Demirbağ

Öz

Bu çalışmada karga (Corvus corone) özofagus, proventrikulus ve gizzard mukozalarındaki glikokonjugatların belirlenmesi amacıyla Triticum vulgare (WGA), Bandeiraea simplicifolia (BSA I-B4), Arachis hypogaea (PNA) ve Ulex europaeus (UEA I) lektinleri kullanıldı. Uygulamalar sonucunda özofagus ve mide (proventrikulus ve gizzard) mukozalarındaki glikokonjugat yoğunluklarının önemli derecede farklılık gösterdiği tespit edildi. Özofagus epitelinin yassı hücre tabakasının α-D-galactosyl, N-acetyl-α-D-galactosaminyl, özofagal bezlerin ise β-Galactoz, Nacetylgalactosamine içeriğinin yoğun olduğu saptandı. Proventrikulus epiteli ve basit bezlerin çok yoğun olarak bileşik bezlerin ise yoğun olarak α-D-galactosyl, N-acetyl-α-D-galactosaminyl içerdiği belirlendi. Bileşik bezlerin aynı zamanda az oranda α-L-Fucose içerdiği dikkati çekti. α-L-Fucose’un gizzardın bazı bez hücrelerinde çok az bulunduğu tespit edildi. Hem proventrikulus hem de gizzardın epitel ve bez hücrelerinde N-acetyl-β-D glucosamine, N-acetylneuraminic acid (siyalik asit), α-Dgalactosyl, N-acetyl-α-D-galactosaminyl içeriklerinin diğer glikokonjugatlara kıyasla daha yoğun miktarda bulunduğu belirlendi.

Anahtar Kelimeler

Gizzard, glikokonjugat, karga, lektin histokimyası, özofagus, proventrikulus

Determination of glycoconjugates in esophagus, proventriculus and gizzard mucosa of crow (Corvus corone) by lectin histochemistry

Öz

In this study, lectins of Triticum vulgare (WGA), Bandeiraea simplicifolia (BSA I-B4), Arachis hypogaea (PNA) and Ulex europaeus (UEA I) were used to determine the glycoconjugates in the esophagus, proventriculus and gizzard mucosa of crow (Corvus corone). As a result of applications the density of glycoconjugate in the mucosa of esophagus and stomach (proventriculus and gizzard) was found to differ significantly. The α-D-galactosyl, N-acetyl-α-D-galactosaminyl content of squamous cell layer of esophagus epithelium, and β-Galactoz, N-acetylgalactosamine content of the esophageal glands were found to be intense. It was determined that the α-D-galactosyl, N-acetyl-α-D-galactosaminyl were included as very intense in the epithelium and simple glands and as intense in compound glands in proventriculus. It was noted that the compound glands also included the α-L-Fucose in small amounts. The α-L-Fucose was slightly in some gland cells of gizzard. It was detected that N-acetyl-β-D glucosamine, N-acetylneuraminic acid (sialic acid), α-D-galactosyl, N-acetyl-α-D-galactosaminyl contents were more intense than the other glycoconjugates in the epithelial and gland cells of both proventriculus and gizzard.

Anahtar Kelimeler

Crow, esophagus, gizzard, glycoconjugate, lectin histochemistry, proventriculus

Kaynakça

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