Sodyum arsenit ve krom (III) klorürün Drosophila melanogaster ’ in eşey oranı ve bazı gelişimsel özellikleri üzerine etkileri

Yıl 2010, Cilt: 26 Sayı: 2, 102 - 111, 01.04.2010

Ayla Karataş Zafer Bahçeci

Öz

Bu araştırmada, sodyum arsenit ve krom (III) klorürün, Drosophila melanogaster’ in ergin bireylerinde, eşey oranı, larva gelişimi ve başkalaşım süresi üzerine etkileri araştırılmıştır. Araştırmada, LC50 değerinin altında olan beş konsantrasyon (0.05, 0.5, 1.0, 2.5 ve 5.0 ml) denenmiştir. Metal çözeltisi Drosophila melanogaster’ e beslenme yoluyla uygulanmıştır. Metal çözeltisi içeren besi yerinde gelişen ergin bireyler, dişi ve erkek eşey ayrımı yapılarak incelenmiştir. Toplam 45163 birey cinsiyet ayrımı yapılarak sayılmıştır. Her iki maddenin hem ayrı hem de birlikte uygulanmasıyla bazı konsantrasyonlarda eşey oranında beklenen 1:1 oranından sapma gözlenmiştir. Eşey oranı, dişi bireylerin oranında artışı ile değişmiş ve erkek bireylerin gelişim sürecinde toksik etkiye karşı daha hassas olduğu bulunmuştur. Larva gelişimi konsantrasyonla doğru orantılı olarak aksamıştır. Özellikle yüksek konsantrasyonlarda gelişim oranı % 35-40 oranına kadar düşmüştür. Fakat başkalaşım süresinde iki nesilde de aksama gözlenmemiştir.

Anahtar Kelimeler

Drosophila, Gelişimsel özellikler, Toksik metaller, Eşey oranı

Effects of sodium arsenite and chromium (ııı) chloride on sex ratio and some development properties of Drosophila melanogaster

Öz

In this research the effects of sodium arsenite and chromium (III) chloride on sex ratio, larval development and metamorphosis duration of Drosophila melanogaster adults have been investigated. In the research five concentrations below LC50 value (0.05, 0.5, 1.0, 2.5, and 5.0 ml) have been tested.Metallic solution has been applied by feeding to Drosophila melanogaster. Adults which developed in medium containing metallic solution have been studied through separating them as females and males. Totally 45163 individuals were counted by gender separation. With application of the two substances both separately and together, deviation from expected 1:1 sex ratio has been observed at some concentrations. Sex ratio has changed as an increase in ratio of female individuals and it has been found that male individuals were more sensitive to toxic effect during development. Larval development failed directly proportional to the concentration. Particularly in high concentrations, development ratio has decreased to 35-40 %. However, in metamorphic duration, no failure has been observed in both generations.

Anahtar Kelimeler

Drosophila, Developmental properties, Toxic metals, Sex ratio

Kaynakça

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