PESTİSİT KALINTI ANALİZLERİNDE ÖRNEK MATRİSİ SORUNU VE ÇÖZÜM YOLLARI

Yıl 2009, Cilt: 25 Sayı: 1, 456 - 478, 01.02.2009

Osman Tiryaki

Öz

Pestisit (bitki koruma ürünü) kalıntı analizlerinde analiz edilecek örnekten ekstraksiyon çözeltisine geçen bileşikler matris etkisi oluşturarak kantitatif analizlerin sonucunu pozitif veya negatif yönde etkileyebilir. Bu çalışmada; güncel literatürlerin ve Avrupa Birliği (AB) direktiflerinin ışığında matris etkisinin mekanizması, bu etki üzerine önemli olan faktörler, matris etkisinin öneminin istatistiksel olarak ortaya konması ve bu sorunun çözüm alternatiflerinin değerlendirilmesi amaçlanmıştır. Matris etkisi sorununa çözüm yolları aranırken; matrisli kalibrasyonun doğrusallığı, metodun hesaplama limiti (LOQ), geri alımları ve tekrar edilebilirlikleri ile birlikte değerlendirilmelidir. Bu olgu her bir pestisit ve matris bazında metot validasyonunu sürecinde rutin olarak değerlendirilmelidir.

Anahtar Kelimeler

Matris etkisi, Matrisli kalibrasyon, Düzeltme faktörü, Analit koruyucu

SAMPLE MATRIX PROBLEM AND ITS SOLUTION IN PESTICIDE RESIDUE ANALYSIS

Öz

In pesticide residue analysis, co-extracted compounds may effect the quantitation of analytical results at both side by inducing matrix-effect. With the update references and European Union (EU) Guides, this review article deals with the mechanism of matrix effect, effecting factors on matrix effect, the statistical significance of it, and alternative aproaches for the solution of matrix problem. While the find out the solution of matrix-induced effect, the assessment should be together with the some other criteria, such as, linearity of calibration, limit of quantiataion (LOQ), recovery, and repeatabilities. This phenomena must be evaluated routinely during the validation of method, based on each pesticide and matrix.

Anahtar Kelimeler

Matrix effect, Matrix-matched calibration, Correction factor, Analyte protectans

Kaynakça

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