BİTKİLERİN YÜKSEK SICAKLIK STRESİNE TOLERANSININ HÜCRE CANLILIĞI VE FOTOSENTETİK PİGMENTASYON TESTLERİ İLE BELİRLENMESİ

Yıl 2007, Cilt: 23 Sayı: 1, 47 - 60, 01.02.2007

Mustafa Yıldız Hakan Terzi

Öz

Stres, olumsuz çevresel faktörlerin üstesinden gelebilmek için bitkinin mücadelesini kapsayan stres toleransı ile yakından ilişkilidir. Yüksek sıcaklık stresi, bitki büyüme ve verimliliğini olumsuz etkileyen bir seri fizyolojik ve biyokimyasal değişikliklere neden olmaktadır. Yüksek sıcaklık koşullarında, birçok organizmanın hayatta kalma ve iyileşme yeteneği “kalıtsal termal tolerans” ve “kazanılan termal tolerans” tarafından belirlenmektedir. Bitki çeşitleri yüksek sıcaklıklara karşı farklı düzeyde tolerans kazanabilirler. Bitki genotiplerinin yüksek sıcaklığa cevap mekanizmaları ve yüksek sıcaklık toleransında bu mekanizmaların rollerinin belirlenmesi önemlidir. Türler ya da çeşitler arasında yüksek sıcaklık toleransı açısından genotipik çeşitliliğin belirlenmesinde, fotosentetik pigment (klorofil a+b ve karotenoidler) birikimi testi ile tetrazolium tuzlarının canlı hücreler tarafından indirgenmesine bağlı hücre canlılığı testi gibi spektrofotometrik-temelli testler kullanılmaktadır.

Anahtar Kelimeler

Bitki, Yüksek sıcaklık stresi, Tolerans, Hücre canlılığı, Fotosentetik pigmentasyon.

DETERMINATION OF TOLERANCE TO HIGH TEMPERATURE STRESS OF PLANTS WITH CELL VIABILITY AND PHOTOSYNTHETIC PIGMENTATION TESTS

Öz

The response mechanisms of plant genotypes to high temperature and determination of roles of these mechanisms in high temperature tolerance are important. The spectrophotometric-based tests such as photosynthetic pigment (chlorophyll a+b and carotenoids) accumulation test, and cell viability test which is related to reduction of tetrazolium salts by viable cells were used in determination of genetic variability in point of high temperature tolerance among species or cultivars.

Anahtar Kelimeler

Plant, High temperature stress, Tolerance, Cell viability, Photosynthetic pigmentation

Kaynakça

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