Futbolcularda Peroksizom Proliferatör – Aktive Reseptör Alfa rs4253778 Polimorfizm Dağılımının Belirlenmesi

Öz

İnsan genom projesinden sonra toplam sayısının 20-25 bin olduğu düşünülen genlerimizden yaklaşık

olarak 250’si atletik performans ile ilişkili olduğu tahmin edilmektedir. Bu gen bölgelerinden dayanıklılık

performansına etki edenlerden biri de peroksizom – proliferatör aktive reseptör alfa (PPARA) rs4253778

polimorfizmidir. Çalışmamızda genç futbolcularda PPARA rs4253778 polimorfizminin dağılımlarını

belirlemeyi amaçladık. Çalışmamıza 64 genç Türk futbolcu katılmıştır. Futbolculardan DNA örnekleri, ağız

içi epitel hücrelerden ticari DNA izolasyon kiti kullanılarak elde edilmiştir. Genotiplendirme işlemi, gerçek

zamanlı polimeraz zincir reaksiyonu (PZR) tekniği ile gerçekleştirilmiştir. Çalışmaya katılan futbolculardan

42’si GG, 21’i GC ve bir futbolcuda CC genotipinde olduğu saptanmıtır. G alleli 105, C alleli ise 23 olarakbelirlenmiştir. Kohortumuzda, patlayıcı güç fenotipi ile ilişkili olan PPARA GG genotipi ve G allelinin

daha baskın olduğu görülmüştür. Futbolcular üzerinde gerçekleştirilen bu pilot çalışmanın bundan sonra

yapılacak olan daha yüksek verili çalışmalara ışık tutacağı inancındayız.

Anahtar Kelimeler

• Futbol,Spor genetiği,Polimorfizm,PPARA

Determination of Peroxisome Proliferator – Activated Receptor Alpha rs4253778 Polymorphism in Football Players

Öz

Approximately 250 regions of our genes, estimated to the total of 20-25 thousand genes, as a rusult of

Human Genome Project, have been associated with athletic performance. One of these gene regions

that affects endurance performance is the peroxisome – activated receptor alpha (PPARA) rs4253778

polymorphism. We aimed to determine the distribution of PPAR polymorphism in young football players.

64 young Turkish football olayers were enrolled to the study. NA isolations were obtained from buccal cells

by using commercial DNA isolation kit. Genotyping is carried out by real-time PCR technique. As a result,

42 of the players were found as GG, 21 players as GC and 1 player as CC genotypes. G allele was counted as

105 and C allele as 23. In our cohort, PPARA GG genotype and G allele, which are associated with power

phenotype, were found to be more dominant. We believe that this pilot study among footballers will guide

for further studies with an extended number of subjects.

Anahtar Kelimeler

• Football,Sports genetics,Polymorphism,PPARA

Kaynakça

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