Profesyonel Voleybolcularda Katekol-O-Metiltransferaz (COMT) rs4680 Polimorfizminin Belirlenmesi

Öz

Katekol-O-Metil Transferaz (COMT) katekolamin nörotransmitterlerin (dopamin, epinefrin,

norepinefrin) inaktivasyonunda rol alır. COMT genindeki keşfedilen fonksiyonel polimorfizmlerin COMT

enzim aktivitesinde değişime sebep olduğu bilinmektedir. Çalışmamızda, COMT geninin Val(108/158)

Met rs4680 polimorfizmi profesyonel voleybolcularda incelenmiş ve COMT rs4680 polimorfizminin

voleybolcularda genotip ve allel dağılımlarının belirlenmesi amaçlanmıştır. Bu kapsamda 21 profesyonel

voleybol sporcumuz çalışmamıza katılmıştır. Genotipleme çalışması Swab DNA izolasyonu sonrasında

gerçek zamanlı polimeraz zincir reaksiyonu (RT-PCR) ile tamamlanmıştır. Araştırmamızın sonuçlarına

göre sporcularımızda AA, AG ve GG genotip dağılımları; 6 sporcumuzda (%29) GG, 7 sporcumuzda (%33)

GA ve 8 sporcumuzsa ise (%38) AA genotipi saptanmıştır. Allellik dağılımlarında ise G Alleli 19 (%45) ve

A Alleli 23(%55) olarak bulunmuştur. Kohortumuzda AA genotipi diğer genotiplere göre, A alleli G alleline

göre daha yüksek bulunmuştur. Bulgularımıza göre voleybol sporcularının düşük aktiviteli AA genotipinin

fazla bulunması voleybol sporuna yatkınlık için biyolojik belirteç olabileceğini kanıtlanmıştır. Çalışmamız

COMT rs4680 polimorfizminin voleybolcularda etkisini inceleyen ilk araştırma niteliğindedir.

Anahtar Kelimeler

• COMT,Voleybol,Genetik,Spor,SNP

Determination of Catechol-O-Methyltransferase (Comt) rs4680 Polymorphism in Professional Volleyball Players

Öz

Catechol-O-Methyl Transferase (COMT) is involved in the inactivation of catecholamine neurotransmitters

(dopamine, epinephrine, norepinephrine). The discovered functional polymorphisms in the COMT

gene are known to cause changes in COMT enzyme activity. In our study, the Val (108/158) Met rs4680

polymorphism of the COMT gene was examined in professional volleyball players and it was aimed to

determine the genotype and allele distributions of COMT rs4680 polymorphism in volleyball players.

In this context, 21 professional volleyball athletes participated in our study. The genotyping study was

completed with real-time polymerase chain reaction (RT-PCR) after swab DNA isolation. According to

the results of our study, AA, AG and GG genotype distributions in our athletes; In 6 of our athletes (29%),

7 of our athletes (33%) had GA and 8 of our athletes (38%) had AA genotype. When we look at the allel

numbers of our volleyball players, G Allele was 19 (45%) and A Allele 23 (55%). AA genotype in our

cohort was higher than other genotypes, compared to A allele G allele. According to our findings, volleyball

athletes’ low activity AA genotype has been proved to be a biomarker for predisposition to volleyball sport.

Our study is the first research to examine the effect of COMT rs4680 polymorphism on volleyball players.

Anahtar Kelimeler

• COMT,Volleyball,Genetics,Sport,SNP

Kaynakça

1. Abe, D., Doi, H., Asai, T., Kimura, M., Wada, T., Takahashi, Y. & Shinohara, K. (2017). Association between COMT Val158Met polymorphism and competition results of competitive swimmers. Journal of Sports Sciences, 36(4), 1–5.
2. Butovskaya, P. R., & Vasilyev, M. L. B. (2013). Molecular-Genetic Polymorphisms of Dopamine, Serotonin and Androgenic Systems as Molecular Markers of Success in Judo Wrestling Sportsmen. Journal of Bioanalysis & Biomedicine, s3(01), 1–6.
3. Breda, V.K., Collins, M., Stein, D. J., & Rauch, L. (2015). The COMT val158met polymorphism in ultraendurance athletes. Physiology & Behavior, 151, 279–283.
4. Corak, A., Kapıcı, S., Sercan, C., Akkoç, O. & Ulucan, K. (2017). A pilot study for determination of anxiety related SLC6A4 promoter “S” and “L” alleles in healthy Turkish athletes. Cellular and Molecular Biology, 63(5), 29-31.
5. Eken, B.F., Akpınaroğlu, C., Arslan, K.S., Sercan, C. & Ulucan, K. (2018). Genlerin sporda psikolojik faktörlerle ilişkisi, The Journal of Neurobehavioral Sciences, 5(1), 56-61.
6. Grossman, M. H., Emanuel, B. S., & Budarf, M. L. (1992). Chromosomal mapping of the human catechol-Omethyltransferase gene to 22q11.1-q11.2. Genomics, 12(4), 822–825.
7. Knöpfli, B., Calvert, R., Bar-Or, O., Villiger, B., & Von Duvillard, S. P. (2001). Competition performance and basal nocturnal catecholamine excretion in cross-country skiers. Medicine and science in sports and exercise, 33(7), 1228–1232.
8. Mc Fie, S., Abrahams, S., Patricios, J., Suter, J., Posthumus, M., & September, A. V. (2018). The association between COMT rs4680 and 5-HTTLPR genotypes and concussion history in South African rugby union players. Journal of Sports Sciences, 36(8), 920–933.

9. Tenhunen, J., Salminen, M., Lundström, K., Kiviluoto, T., Savolainen, R., & Ulmanen, I. (1994). Genomic organization of the human catechol O-methyltransferase gene and its expression from two distinct promoters. European journal of biochemistry, 223(3), 1049–1059.
10. Ulucan, K., Yalcin, S., Akbas, B., Uyumaz, F., & Konuk, M. (2014). Analysis of Solute Carrier Family 6 Member 4 Gene promoter polymorphism in young Turkish basketball players. The Journal of Neurobehavioral Sciences, 1(2), 37.
11. Ulucan, K., Topal, E.S., Aksulu, B.K., Yaman, B., Çiftçi, İ.C., & Türker Bıyıklı. (2015). Atletik Performans, Genetik ve Gen Dopingi. İKSST Derg, 7(2),58-62.