Preparation and Characterization of Chitosan/κ-Carrageenan Based Polymeric Nanoparticles for Gemcitabine Delivery

Öz

Cancer is the most fatal disease of the last century after cardiovascular disease. Gemcitabine is a nucleoside
analogue used in the treatment of cancer. However, like many anticancer drugs, it has many side effects that
limit treatment. A nano drug delivery system has been developed to provide effective treatment by reducing
side effects. Chitosan and κ-carrageenan, which are regarded as safe by the FDA, were used in the preparation
of the nano drug delivery system. In the synthesis, pre-ionic gelation followed by polyelectrolyte complexation
method was used and then a second ionic gelation step was added. Chitosan: κ-carrageenan ratio and CaCl2
concentration were optimized and the optimum polymer ratio was determined as 6:1 and CaCl2 concentration
was 2.5%. Its hydrodynamic size at optimum conditions was 393 nm and its size measured size in TEM was 20
nm. FTIR analyzes showed that nanoparticle synthesis was successful. Drug loading was performed by
encapsulation and 58% drug loading was achieved. After drug loading, the hydrodynamic dimension was
increased to 595 nm and its size measured size in TEM 45 nm. Drug release was monitored for 95 hours and
was determined to be higher at pH 6.0 compared to 7.4 and pH sensitive. In addition, the Higuchi model is the
most suitable mathematical model for drug release kinetics. The obtained results showed that chitosan: κcarrageenan nanoparticles were suitable for gemcitabine delivery and were pH sensitive enough to respond to
the tumor microenvironment.

Anahtar Kelimeler

• chitosan
• kappa-carrageenan
• gemcitabine
• drug delivery
• nanoparticle
• chitosan, kappa-carrageenan, gemcitabine, drug delivery, nanoparticle

Kaynakça

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