Araştırma Makalesi
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Salvia microstegia Merikarplarının Antimikrobiyal ve Antibiyofilm Aktiviteleri Üzerine Bir Araştırma

Yıl 2022, , 649 - 658, 31.08.2022
https://doi.org/10.18185/erzifbed.1136242

Öz

Biyofilm ilişkili enfeksiyonlar tüm dünyada önemli sağlık sorunlarına neden olmakatadır. Antimikrobiyallere karşı gelişen direnç ve olası yan etkiler nedeniyle, araştırmacıların dikkati bitki ekstreleri ve onların bileşenleri üzerine yoğunlaşmıştır. Bu çalışmanın amacı Salvia microstegia Boiss. et Bal. merikarplarının antimikrobiyal aktivite ve antibiyofilm potansiyellerini değerlendirmektir. Merikarplardan elde edilen etanol ekstresinin antimikrobiyal aktivitesi yedi standart bakteri ve üç standart maya suşuna karşı mikrodilüsyon yöntemi kullanılarak belirlenmiştir. Ayrıca ekstrenin Pseudomonas aeruginosa’nın biyofilm oluşumunu inhibe etme ve oluşmuş biyofilmi üzerine etkisi kristal viyole yöntemi kullanılarak belirlenmiştir. Kullanılan ekstre, 62.5–250 µg/mL aralıkta minimum inhibisyon konsantrasyonu (MİK) değerleri ile test edilen bakteri ve mayaları karşı orta ve düşük antimikrobiyal aktivite göstermiştir. Bununla birlikte, ekstrenin önceden oluşturulmuş biyofilmleri inhibe etme potansiyelinin biyofilm oluşumunu engelleme potansiyelinden daha iyi olduğu tespit edilmiştir. Sonuç olarak, Salvia microstegia merikarplarından elde edilen ekstrenin antibiyofilm potansiyeli bulunmakla beraber, antimikrobiyal potansiyelinin daha geniş çerçevede ortaya konabilmesi için farklı suşların dâhil edildiği ilave çalışmalara ihtiyaç vardır.

Kaynakça

  • Adımcılar, V., Kalaycıoğlu, Z., Aydoğdu, N., Dirmenci, T., Kahraman, A. & Erim, F. B., 2019. “Rosmarinic and carnosic acid contents and correlated antioxidant andantidiabetic activities of 14 Salvia species from Anatolia”. Journal of Pharmaceutical and Biomedical Analysis 175, 112763-68.
  • Alekshun, M. N. and Levy, S. B., 2007. “Molecular mechanisms of antibacterial multidrug resistance,” Cell, 128(6),1037– 1050. Bakoğlu, A., Kılıç, O. & Kökten, K., 2016. “Fatty acid composition of the leaves of some Salvia taxa from Turkey”. Chemistry of Natural Compounds 52(4), 676-678.
  • Bayar, Y. & Genç, N., 2018. “Determination of the chemical components, antioxidant and antifungal activities of essential oil and plant extract of Salvia candidissima Vahl.” Mediterranean Agricultural Sciences 31(2), 93-99.
  • Ben Haj Khalifa, A., Moissenet, D., Vu Thien, H., Khedher, M., 2011. “Virulence factors in Pseudomonas aeruginosa: mechanisms and modes of regulation”. Annales de biologie clinique 69(4),393–403.
  • Bjarnsholt, T. 2013., “The role of bacterial biofilms in chronic infections”. APMIS Suppl. 136,1-51.
  • Del Pozo, J. L. 2018., “Biofilm-related disease”, Expert Rev Anti Infect Ther. 16(1), 51-65.
  • Drew, B. T., González-Gallegos, J. G., Xiang, C. L., Kriebel, R., Drummond, C. P. & Walker, J., 2017. “Salvia united: the greatest good for the greatest number”. Taxon 66,133-145.
  • Grant, S. S. and Hung, D. T. 2013., “Persistent bacterial infections,antibiotic tolerance, and the oxidative stress response,” Virulence, 4(4),273–283.
  • Güzel, S., Özay, Y., Ülger, M., Kahraman, A., Aslan, G., 2018. “Evaluation of Antimicrobial Activity of Three Endemic Salvia Species Growing Wild in Turkey”. Hacettepe University Journal of the Faculty of Pharmacy, 38(1), 4-10.
  • Güzel, S., Kahraman, A.,; Ülger, M., Özay, Y., Bozgeyik, I., Sarıkaya, Ö., 2019. “Morphology, myxocarpy, mineral content, and in vitro antimicrobial and antiproliferative activities of mericarps of the vulnerable Turkish endemic Salvia pilifera”. Journal of Research in Pharmacy, 23,729-739.
  • Güzel, S., Özay, Y., Kumaş, M., Uzun, C., Özkorkmaz, E., Yıldırım, Z., Ülger, M., Güler, G., Çelik, A., Çamlıca, Y., Kahraman, A., 2019. “Wound healing properties, antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats”. Bıomedıcıne & Pharmacotherapy, 11,1260-1276.
  • Güzel, S., Ülger, M., Kahraman, A. 2020., “Chemical composition and some biological activities of Salvia longipedicellata Hedge mericarps”. Chemistry of Natural Compounds, 56(5), 788-792.
  • Güzel, S., Ülger, M., Kahraman, A., 2021. “Phytochemical analysis, antioxidant and antimicrobial activities of Salvia virgata mericarps”. Botanica Serbica, 45(2), 223-231.
  • Hatipoglu, S. D., Zorlu, N., Dirmenci, T., Goren, A. C., Ozturk, T. & Topcu, G., 2016. “Determination of volatile organic compounds in fourty five Salvia species by thermal desorption-GC-MS technique”. Records of Natural Products 10(6): 659-700.
  • Hengzhuang, W., Wu, H., Ciofu, O., Song, Z., Hoiby, N., 2012. “In vivo pharmacokinetics/pharmacodynamics of colistin and imipenem in Pseudomonas aeruginosa biofilm infection”. Antimicrobial Agents Chemotherapy 56,2683-90.
  • Ibberson, C. B., 2020. “Whiteley, M. The social life of microbes in chronic infection”, Curr. Opin. Microbiol. 53,44–50.
  • Jorgensen, J. H., Ferraro. M. J. 1998. “Antimicrobial susceptibility testing: general principles and contemporary practice”. Clin Infect Dis 26,973–80.
  • Kartal, M., 2007. "Intellectual property protection in the natural product drug discovery, traditional herbal medicine and herbal medicinal products". Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 21(2), 113-119.
  • Kürşat, M., Erecevit, P., Sarı, A., Emre, İ., Kırbağ, S. & Civelek, Ş., 2012. “The antimicrobial activities of seed fatty acid extracts from some Salvia L. Species”. Turkish Journal of Science & Technology 7(1),31-36.
  • Lindsay, D., von Holy, A., 2006. “Bacterial biofilms within the clinical setting: what healthcare professionals should know”. J Hosp Infect. 64(4),313-25.
  • Nascimento, G. G., Locatelli, J., Freitas, P. C., & Silva, G. L., 2000. "Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria". Brazilian Journal of Microbiology, 31(4), 247-256.
  • Macia, M. D., Rojo-Molinero, E., Oliver, A., 2014. “Antimicrobial susceptibility testing in biofilm-growing bacteria”. Clin Microbiol Infect. 20(10),981-90.
  • Melandera, R. J., Basaka, A. K., Melandera, C., 2020. “Natural Products as Inspiration for the Development of Bacterial Antibiofilm Agents”. Nat Prod Rep. 37(11),1454–1477.
  • Morrison, L., Zembower, T.R., 2020. “Antimicrobial Resistance”. Gastrointest Endosc Clin N Am. 30(4):619-635. O’Toole, G. A., 2011. “Microtiter dish biofilm formation assay”. J Vis Exp 47,2437.
  • Pitarokili D, Tzakou O & Loukis A. 2006. “Essential oil composition of Salvia verticillata, S. verbenaca, S. glutinosa and S. candidissima growing wild in Greece”. Flavour and Fragrance Journal 21,670-673.
  • Tekintaş, Y., Temel, A., Ateş, A., Eraç, B., Metin, D.Y., Hilmioğlu Polat, S,, Hoşgör Limoncu, M. 2020. “Antifungal and Antibiofilm Activities of Selective Serotonin Reuptake Inhibitors Alone and in Combination with Fluconazole”. Turk J Pharm Sci. 17(6),667-672.
  • Thi, M. T. T., Wibowo, D., Rehm, B. H. A., 2020. “Pseudomonas aeruginosa biofilms”. Int J Mol Sci 21,8671.
  • Topçu, G., Yücer, R. & Şenol, H., 2017. Bioactive constituents of Anatolian Salvia species. In: GEORGIEV V & Pavlov A. (ed), , 2017. Salvia Biotechnology. pp 31-132, Springer International Publishing AG. Springer, Cham.
  • Vasudevan, R., 2014. “Biofilms: microbial cities of scientific significance,” Journal of Microbiology & Experimentation, 1(3), 1–16.
  • Woods, G. L., Washington, J. A., 1995. Antibacterial susceptibility tests: dilution and disk diffusion methods. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, (eds). Manual of clinical microbiology. 6th ed, Washington, DC: American Society for Microbiology. 1327–41.
  • Yapar, Y., Behçet, L., 2018 “The flora of Hiro Plateau (Adaklı-Bingöl/ Turkey) and its surroundings”. Biological Diversity and Conservation .11(3),126-140.
  • Zhong, H., Xie, Z., Wei, H., Zhang, S., Song, Y., Wang, M., and Zhang, Y., 2019. “Antibacterial and Antibiofilm Activity of Temporin-GHc and Temporin-GHd Against Cariogenic Bacteria, Streptococcus mutans”. Front Microbiol. 10, 2854.

Study on Antimicrobial and Antibiofilm Activities of Salvia microstegia mericarps from Turkish flora

Yıl 2022, , 649 - 658, 31.08.2022
https://doi.org/10.18185/erzifbed.1136242

Öz

Abstract
Biofilm-associated infections cause important health problems all over the world. Because of the resistance to antimicrobials and their possible side effects, researchers' attention has been focused on plant extracts and their components. The aim of this study is to evaluate the antimicrobial and antibiofilm potentials of Salvia microstegia Boiss. et Bal. mericarps. The antimicrobial activity of ethanolic extract obtained from the mericarps was evaluated using the microdilution method against seven standard bacteria and three standard yeast strains. In addition, the effect of the extract on inhibiting biofilm formation and preformed biofilm of Pseudomonas aeruginosa was determined using the crystal violet method. The used extract showed moderate to low antimicrobial activity against tested bacteria and yeasts with minimum inhibitory concentration (MIC) values ranging from 62.5 to 250 µg/mL. Moreover, the extract's potential to inhibit preformed biofilms was found to be greater compared to its potential to inhibit biofilm formation. In conclusion, the tested extract obtained from Salvia microstegia mericarps showed antibiofilm potential, however, additional studies involving different strains are needed to reveal its antimicrobial potential in a more holistic perspective.

Kaynakça

  • Adımcılar, V., Kalaycıoğlu, Z., Aydoğdu, N., Dirmenci, T., Kahraman, A. & Erim, F. B., 2019. “Rosmarinic and carnosic acid contents and correlated antioxidant andantidiabetic activities of 14 Salvia species from Anatolia”. Journal of Pharmaceutical and Biomedical Analysis 175, 112763-68.
  • Alekshun, M. N. and Levy, S. B., 2007. “Molecular mechanisms of antibacterial multidrug resistance,” Cell, 128(6),1037– 1050. Bakoğlu, A., Kılıç, O. & Kökten, K., 2016. “Fatty acid composition of the leaves of some Salvia taxa from Turkey”. Chemistry of Natural Compounds 52(4), 676-678.
  • Bayar, Y. & Genç, N., 2018. “Determination of the chemical components, antioxidant and antifungal activities of essential oil and plant extract of Salvia candidissima Vahl.” Mediterranean Agricultural Sciences 31(2), 93-99.
  • Ben Haj Khalifa, A., Moissenet, D., Vu Thien, H., Khedher, M., 2011. “Virulence factors in Pseudomonas aeruginosa: mechanisms and modes of regulation”. Annales de biologie clinique 69(4),393–403.
  • Bjarnsholt, T. 2013., “The role of bacterial biofilms in chronic infections”. APMIS Suppl. 136,1-51.
  • Del Pozo, J. L. 2018., “Biofilm-related disease”, Expert Rev Anti Infect Ther. 16(1), 51-65.
  • Drew, B. T., González-Gallegos, J. G., Xiang, C. L., Kriebel, R., Drummond, C. P. & Walker, J., 2017. “Salvia united: the greatest good for the greatest number”. Taxon 66,133-145.
  • Grant, S. S. and Hung, D. T. 2013., “Persistent bacterial infections,antibiotic tolerance, and the oxidative stress response,” Virulence, 4(4),273–283.
  • Güzel, S., Özay, Y., Ülger, M., Kahraman, A., Aslan, G., 2018. “Evaluation of Antimicrobial Activity of Three Endemic Salvia Species Growing Wild in Turkey”. Hacettepe University Journal of the Faculty of Pharmacy, 38(1), 4-10.
  • Güzel, S., Kahraman, A.,; Ülger, M., Özay, Y., Bozgeyik, I., Sarıkaya, Ö., 2019. “Morphology, myxocarpy, mineral content, and in vitro antimicrobial and antiproliferative activities of mericarps of the vulnerable Turkish endemic Salvia pilifera”. Journal of Research in Pharmacy, 23,729-739.
  • Güzel, S., Özay, Y., Kumaş, M., Uzun, C., Özkorkmaz, E., Yıldırım, Z., Ülger, M., Güler, G., Çelik, A., Çamlıca, Y., Kahraman, A., 2019. “Wound healing properties, antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats”. Bıomedıcıne & Pharmacotherapy, 11,1260-1276.
  • Güzel, S., Ülger, M., Kahraman, A. 2020., “Chemical composition and some biological activities of Salvia longipedicellata Hedge mericarps”. Chemistry of Natural Compounds, 56(5), 788-792.
  • Güzel, S., Ülger, M., Kahraman, A., 2021. “Phytochemical analysis, antioxidant and antimicrobial activities of Salvia virgata mericarps”. Botanica Serbica, 45(2), 223-231.
  • Hatipoglu, S. D., Zorlu, N., Dirmenci, T., Goren, A. C., Ozturk, T. & Topcu, G., 2016. “Determination of volatile organic compounds in fourty five Salvia species by thermal desorption-GC-MS technique”. Records of Natural Products 10(6): 659-700.
  • Hengzhuang, W., Wu, H., Ciofu, O., Song, Z., Hoiby, N., 2012. “In vivo pharmacokinetics/pharmacodynamics of colistin and imipenem in Pseudomonas aeruginosa biofilm infection”. Antimicrobial Agents Chemotherapy 56,2683-90.
  • Ibberson, C. B., 2020. “Whiteley, M. The social life of microbes in chronic infection”, Curr. Opin. Microbiol. 53,44–50.
  • Jorgensen, J. H., Ferraro. M. J. 1998. “Antimicrobial susceptibility testing: general principles and contemporary practice”. Clin Infect Dis 26,973–80.
  • Kartal, M., 2007. "Intellectual property protection in the natural product drug discovery, traditional herbal medicine and herbal medicinal products". Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 21(2), 113-119.
  • Kürşat, M., Erecevit, P., Sarı, A., Emre, İ., Kırbağ, S. & Civelek, Ş., 2012. “The antimicrobial activities of seed fatty acid extracts from some Salvia L. Species”. Turkish Journal of Science & Technology 7(1),31-36.
  • Lindsay, D., von Holy, A., 2006. “Bacterial biofilms within the clinical setting: what healthcare professionals should know”. J Hosp Infect. 64(4),313-25.
  • Nascimento, G. G., Locatelli, J., Freitas, P. C., & Silva, G. L., 2000. "Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria". Brazilian Journal of Microbiology, 31(4), 247-256.
  • Macia, M. D., Rojo-Molinero, E., Oliver, A., 2014. “Antimicrobial susceptibility testing in biofilm-growing bacteria”. Clin Microbiol Infect. 20(10),981-90.
  • Melandera, R. J., Basaka, A. K., Melandera, C., 2020. “Natural Products as Inspiration for the Development of Bacterial Antibiofilm Agents”. Nat Prod Rep. 37(11),1454–1477.
  • Morrison, L., Zembower, T.R., 2020. “Antimicrobial Resistance”. Gastrointest Endosc Clin N Am. 30(4):619-635. O’Toole, G. A., 2011. “Microtiter dish biofilm formation assay”. J Vis Exp 47,2437.
  • Pitarokili D, Tzakou O & Loukis A. 2006. “Essential oil composition of Salvia verticillata, S. verbenaca, S. glutinosa and S. candidissima growing wild in Greece”. Flavour and Fragrance Journal 21,670-673.
  • Tekintaş, Y., Temel, A., Ateş, A., Eraç, B., Metin, D.Y., Hilmioğlu Polat, S,, Hoşgör Limoncu, M. 2020. “Antifungal and Antibiofilm Activities of Selective Serotonin Reuptake Inhibitors Alone and in Combination with Fluconazole”. Turk J Pharm Sci. 17(6),667-672.
  • Thi, M. T. T., Wibowo, D., Rehm, B. H. A., 2020. “Pseudomonas aeruginosa biofilms”. Int J Mol Sci 21,8671.
  • Topçu, G., Yücer, R. & Şenol, H., 2017. Bioactive constituents of Anatolian Salvia species. In: GEORGIEV V & Pavlov A. (ed), , 2017. Salvia Biotechnology. pp 31-132, Springer International Publishing AG. Springer, Cham.
  • Vasudevan, R., 2014. “Biofilms: microbial cities of scientific significance,” Journal of Microbiology & Experimentation, 1(3), 1–16.
  • Woods, G. L., Washington, J. A., 1995. Antibacterial susceptibility tests: dilution and disk diffusion methods. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, (eds). Manual of clinical microbiology. 6th ed, Washington, DC: American Society for Microbiology. 1327–41.
  • Yapar, Y., Behçet, L., 2018 “The flora of Hiro Plateau (Adaklı-Bingöl/ Turkey) and its surroundings”. Biological Diversity and Conservation .11(3),126-140.
  • Zhong, H., Xie, Z., Wei, H., Zhang, S., Song, Y., Wang, M., and Zhang, Y., 2019. “Antibacterial and Antibiofilm Activity of Temporin-GHc and Temporin-GHd Against Cariogenic Bacteria, Streptococcus mutans”. Front Microbiol. 10, 2854.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Zehra Öksüz 0000-0002-1542-0556

Sevda Güzel 0000-0002-6642-5824

Ahmet Kahraman 0000-0002-9344-1993

Yayımlanma Tarihi 31 Ağustos 2022
Yayımlandığı Sayı Yıl 2022

Kaynak Göster

APA Öksüz, Z., Güzel, S., & Kahraman, A. (2022). Study on Antimicrobial and Antibiofilm Activities of Salvia microstegia mericarps from Turkish flora. Erzincan University Journal of Science and Technology, 15(2), 649-658. https://doi.org/10.18185/erzifbed.1136242