Öz

Scrophularia fatmae Kandemir & İlhan ve Scrophularia erzincanica R.R. Mill (Scrophulariaceae) Erzincan/Türkiye'ye özgü endemik türlerdir. S. erzincanica ve S. fatmae'de ki IUCN kategorileri sırasıyla EN ve CR'dir. Bu çalışmada, ISSR belirteçlerini kullanarak Erzincan'daki üç S. erzincanica popülasyonunun ve iki S. fatmae popülasyonunun genetik varyasyon seviyelerini ve genetik yapısı araştırılmıştır. Bu amaçla 10 primer, 75 bireyden oluşan beş popülasyondan 104'ü (%89.6) polimorfik olmak üzere toplam 116 bandı amplifiye edildi. UPGMA küme analizi, genetik varyasyonlar ve coğrafi mesafeler arasında anlamlı bir ilişki olduğunu göstermiştir. Alpin bölgesine uyum sağlamış olan S. fatmae'nin yayılış alanı ve populasyon büyüklüğü daha küçüktür. Ek olarak, S. fatmae'nin kendi kendine tozlaşma eğilimi daha yüksektir. S. erzincanica daha büyük bir popülasyona sahiptir. S. erzincanica ve S. fatmae'de etkili tozlayıcı sayısı sırasıyla üç ve birdir. Bu sebeplerin de katkısı ve araştırma sonuçlarına göre S. fatmae ve S. erzincanica türleri karşılaştırıldığında S. erzincanica'da genetik çeşitliliğin daha fazla olduğu görülmektedir. Bu çalışma sonucunda elde edilen genetik veriler, S. erzincanica ve S. fatmae türlerinin yanı sıra diğer nadir ve tehlike altındaki bitki türleri için koruma stratejilerinin geliştirilmesinde kullanılabilir.

Proje Numarası

218Z002

Genetic Diversity Analyses of Scrophularia erzincanica and Scrophularia fatmae (Scrophulariaceae) Populations Distributed in Eastern Anatolia of Türkiye

Öz

Scrophularia fatmae Kandemir & İlhan and Scrophularia erzincanica R.R. Mill (Scrophulariaceae) are endemic species to Erzincan/ Turkiye. IUCN categories in S. erzincanica and S. fatmae are EN and CR. In the present study, we investigated levels of genetic variation and genetic structure of three populations of S. erzincanica, and two populations of S. fatmae in Erzincan using ISSR markers. For this aim, 10 primers amplified 116 total bands, with 104 (89.6 %) being polymorphic, from five populations composed of 75 individuals. The UPGMA cluster analysis demonstrated a significant correlation between genetic variations and geographic distances. The distribution area and population size of S. fatmae, which has adapted to the alpine region, is smaller. In addition, S. fatmae has a higher tendency to self-pollination. S. erzincanica has a larger population. The number of effective pollinators in S. erzincanica and S. fatmae are three and one, respectively. With the contribution of these reasons, when we compare S. fatmae and S. erzincanica species according to the results of the research, it is seen that genetic diversity is higher in S. erzincanica. The genetic data obtained as a result of present study may be used in the development of conservation strategies for other rare and endangered plant species, in addition to S. erzincanica and S. fatmae species.

Anahtar Kelimeler

• Endemic plant
• ISSR
• Population genetic
• S. erzincanica
• S. fatmae

Destekleyen Kurum

TÜBİTAK

Proje Numarası

218Z002

Teşekkür

The research was carried out with the support of TÜBİTAK within the scope of the 1001 project numbered 218Z002. We thank TÜBİTAK for their support.

Kaynakça

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