Mitigating Osmotic Drought Stress in Rye: Assessing the Ameliorative Effects of Putrecine on Germination, Seedling and Mitotic Index

Öz

Abstract In this study, the impact of putrescine, a polyamine, on the germination, seedling growth, and mitotic index of rye seeds (cv. Aslım-95) was investigated under osmotic drought conditions induced by polyethylene glycol (PEG-6000) application. The experiment involved six different PEG doses (-2, -4, -6, -8, and -10 bars) in combination with putrescine doses of 0.001, 0.01, and 0.1 mM. The concentration of -2 bars of polyethylene glycol, representing osmotic drought, positively influenced some of the studied characteristics. However, the stimulative effect of -2 bars of osmotic drought exhibited minimal changes with PEG 6000 + putrescine combinations. Conversely, as the osmotic drought level (PEG 6000 concentration) increased beyond that concentration, all the observed characteristics were negatively impacted. All putrescine doses used in combination with PEG 6000 demonstrated positive effects on the examined characteristics and reduced the germination duration. The most significant effect of putrescine was observed at a concentration of 0.1 mM on the base dry weight and 0.001 mM concentration on all other characteristics. Consequently, high doses of osmotic drought stress, particularly, adversely affected germination and seedling growth. It was determined that all putrescine doses could partially alleviate the negative effects induced by osmotic drought caused by PEG 6000.

Anahtar Kelimeler

• Rye
• PEG 6000
• putrescine
• germination
• seedling
• mitotic index

Kaynakça

1. [1] Kün, E., Çiftçi, CY., Birsin, M., Ülger, AC., Karahan. S., Zencirci. N., Öktem. A., Güler, M., Yılmaz, N., Atak, M. (2004) Tahıl ve yemeklik baklagiller üretimi. Türkiye Ziraat Mühendisliği VI. Teknik Kongresi - 31.12.2004.
2. [2] Anonim. (2022) https://data.tuik.gov.tr/Kategori/GetKategori?p=Tarim_11.12.01.2024
3. [3] Khan, NA., Singh, S. (2008) Abiotic Stress and Plant Responses. IK Internarional. New Delhi.
4. [4] Saxena, NP., Johansen. C, Saxena, MC., Silim, SN. (1993) Selection for drought and salinity tolerance in cool-season food legumes. Breeding for stress tolerance in cool-season food legumes. Eds: K.B. Singh and M.C. Saxena. United Kingdom. 245-270.
5. [5] Mooring, M.T., Cooper, AW., Senaca. ED. (1971) Seed germination response and evidence for height ecophenes in Spartina alterniflora from North Carolina. American Journal of Botany, 58: 48-55.
6. [6] Bozcuk, S. (1978) Domates (Lycopersicum esculentum Mill), arpa (Hordeum vulgare L.) ve pamuk (Gossypium hirsitum L.) bitkilerinin büyüme ve gelişmesinde tuz kinetin etkileşimi üzerinde araştırmalar. Doçentlik tezi. Hacettepe Üniversitesi. Fen Fakültesi. Botanik Bölümü. Ankara.
7. [7] Pan, XY., Wang, YF., Wang, GX., Cao, QD., Wang, J. (2002) Relationship between growth redundancy and size inequality in spring wheat populations mulched with clear plastic film. Acta Phytopathologica Sinica, 26: 177-184.
8. [8] Rauf, M., Munir. M., Ul-Hassan, M., Ahmed., M., Afzal, M. (2007) Performance of heat genotypes under osmotic stress at germination and early seedling growth stage. African Journal of Biotechnology, 6 (8): 971-975.

9. [9] Dilday, R., Mgonja, M., Amonsilpa, S., Collins, F., Wells, B. (1990) Plant height vs. mesocotyl and celeoptile elongation in rice: linkage or pleiotropism? Crop Science. 30 (4): 815-818.
10. [10] Lagerwerff, J., Ogata, G., Eagle, HE. (1961) Control of osmotic pressure of culture solutions with polyethylene glycol. Science, 133 (3463): 1486-1487.
11. [11] Gupta, S., Gupta, N. (2011) Field efficacy of exogenously applied putrescine in wheat (Triticum aestivum L.) under water-stress conditions. Indian Journal of Agricultural Sciences, 81(6): 516-9.
12. [12] Duncan, DB. (1955). Multiple range and multiple F tests. Biometrics, 11(1): 1-42.
13. [13] Michel, BE., Kaufmann, MR., (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51 (5): 914-916.
14. [14] Elkoca, E., (1997) Fasulye (Phaseolus vulgaris L)’de tuza dayanıklılık üzerine bir araştırma. Atatürk Üniversitesi Fen Bilimleri Enstitüsü. Yüksek Lisans Tezi. Erzurum.
15. [15] Maguire, JD., (1962) Speed of germination aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2 (2): 176-177.
16. [16] Aydın, M., Hossein Pour, A., Haliloğlu, K., Tosun, M. (2015) Effect of application of putrescine on seedling growth and cell division of wheat (Triticum aestivum L.) under drought stress. Yuzuncu Yıl University Journal of Agricultural Sciences, 26(3): 319-332.
17. [17] Abdul-Baki, AA., Anderson, JD. (1970) Viability and leaching of sugars from germinating barley. Crop Science, 10 (1): 31-34.
18. [18] Kaya, MD., Okçu, G., Atak, M., Çıkılı, Y., Kolsarıcı, Ö. (2006) Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24(4): 291-295.
19. [19] Hossein Pour, A. (2016) Kimyasal mutagen uygulamalarının buğdayda bazı invitro karakterler ve kuraklığa tolerans üzerine etkileri. Atatürk Üniversitesi Fen Bilimleri Enstitüsü Tarla Bitkileri Ana Bilim Dalı, Doktora Tezi. 57 s.
20. [20] Sagsöz, S. (1974) Diploid İngiliz çiminden tetraploid İngiliz çiminin elde edilmesi imkanları. Bu bitkilerde mitoz ve meioz kromozomları ile bazı morfolojik özelliklerin mukayesesi. Atatürk Üniv. Yay. No: 325. Zir. Fak. Yay. No: 159. Araş. Serisi No: 95. Erzurum.
21. [21] Tosun, M. (1995) Heksaploid triticale çeşit/hatlarında tane verimini etkileyen bazı sitolojik ve morfolojik özelliklerin belirlenmesi. Atatürk Üniversitesi Fen Bilimleri Enstitüsü Tarla Bitkileri Ana Bilim Dalı, Doktora Tezi. 112 s.
22. [22] Reiger, R., Michaelis, A., Green, MM. (1968) A. Glassory Genetics and Cytogenetics. Springer-Verbig, 507 p.
23. [23] Lafond, GP., Fowler, BD. (1989) Soil temperature and water content. seedling depth. and simulated rainfall effects on winter wheat emergence. Agronomy Journal, 81: 609-614.
24. [24] Taskesenligil, B. (2016) Düşük su potansiyelinde çimlenme hücre membran hasarı ve paraquata tolerans ölçütleri ile buğday genotiplerinin erken dönem kuraklığa dayanıklılık yönünden karakterizasyonu. Atatürk Üniversitesi Fen Bilimleri Enstitüsü Tarla Bitkileri Ana Bilim Dalı, Yüksek Lisans Tezi. Erzurum.
25. [25] Dhanda, SS., Sethi, GS., Behl, RK. (2004) Indices of drought tolerance in wheat genotypes at early stages of plant growth. Journal of Agronomy and Crop Science, 190 (1): 6-12.
26. [26] Baloch, MS., Nadim, MA., Zubair, M., Awan, IU., Khan, EA., Ali, AS. (2012) Evaluation of wheat under normal and late sowing conditions. Pakistan Journal Botany, 44 (5): 1727-1732.
27. [27] Pius, J., George, L., Eapen, S., Rao, P. (1994) Evaluation of somaclonal and mutagen induced variation in fingermillet. Plant Breeding, 112 (3): 239-243.
28. [28] Blum, A., Sinmena, B., Zıvr, O. (1980) An evaluation of seed and seedling drought tolerance screening tests in Wheat. Euphytica, 29 (3): 727-736.
29. [29] Gholamin, R., Khayatnezhad, M., Somarin, S., Mahmoodabad, RZ. (2010) Effects of plyethylene gycol and NACI stress on two cutiwars of wheat (Tritucum durum) at germination and early seedling stages. American Eurasian Journal of Agricutural and Enviromental Science, 9 (1): 86-90.
30. [30] Rezzaq, A., Ali, Q., Qayyum, A., Mahmood, MA., Rasheed, M. (2013) Physiological responses and drought resiistance index of nine wheat (Triticum aestivum L.) cultivars under different moisture conditions. Pakistan Jornal of Botany, 45 (S1): 151-155.
31. [31] Almansouri, M., Kinet, JM., Lutts, S. (2001) Effect of salt and osmotic stresses on germination in durum wheat (Tritucum durum Desf.). Plant and Soil, 231 (2): 243-254.
32. [32] Akkuş, G., (2009) Farklı dozlardaki (GA3) ve IBA hormonlarının bazı standart antep fıstığı (Pistacia vera L.) tohumlarının çimlenme oranı ve hızı üzerine etkisi. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Bahçe Bitkileri Ana Bilim Dalı, Yüksek Lisans Tezi. Van.
33. [33] Hızarcı, Y., (2001) Karaerik üzüm çeşidinde katlama ve GA3 uygulamalarının tohum çimlenmesi ve dinlenmesi üzerine etkileri. Atatürk Üniversitesi Fen Bilimleri Enstitüsü Bahçe Bitkileri Anabilim Dalı, Yüksek lisans tezi. 54 s.
34. [34] Naylor, REL., Gurmu, M. (1990) Seed vigour and water relations in wheat. Annals of Applied Biology, 117 (2): 441-450.
35. [35] Barutçular, C., Koç, M., Genç, İ. (1994) Ekmeklik buğdayda çimlenme ve ilk büyümenin PEG-6000 yardımı ile oluşturulan su geriliminden etkilenişi. Türkiye I. Tarla Bitkileri Kongresi. İzmir.
36. [36] Jajarmi, V. (2009) Effect of water stress on germination indices in seven wheat cultivar. World Academy of Science. Engineering and Tecnology, 49: 105-106.
37. [37] Ahmadizadeh, M., Valizadeh, M., Zaefizadeh, M., Shahbazi, H. (2011). Evaluation of interaction between genotype and environments in term of germination and seedling growth in durum wheat landraces. Advances in Environmental Biology, 5 (4): 551-558.
38. [38] Ugur, Ö. (2015) Kuraklık stresinin arpada ilk gelişme dönemi üzerine etkileri. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Tarla Bitkileri Anabilim Dalı, Yüksek Lisans Tezi. Van.
39. [39] Valero, D. Perez-Vicente, A. Martinez-Rormero, D., Castillo, S., Guıllen, F., Serrano, M., (2002) Plum Storahility imtoved after calcium and Heat posthatvest trearments: role of polyamınes. Journal of Food Science, 67: 2571-3.
40. [40] Bonchetau, A., Aziz, A., Larher, F., Martin-Tanguy, J. (1999) Polyamines and environmental challenges: Recent Development Plant science, 140: 103-25.
41. [41] Groppa, MD., Benavides, MP. (2008). Polyamines and abiotic Stress: reccent advances. Amino acids, 34: 35-45.
42. [42] Liu, JH., Kitashiba, H., Wang, J., Ban, Y., Moriguchi, T. (2007) Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnology, 24: 117-26.
43. [43] Garcia-Jimenez, P., Just, PM., Delgado, AM., Robaina, RR. (2007) Transglutaminase activity decrease duting acclimation to hyposaline conditions in marine seaweed Gratelonpiadoryphora (Rhodophayta. Halymeniaceae). Journal of plant physiology, 164: 367-70.
44. [44] He, L., Ban, Y., İnoue, H., Matsuda, N., Liu, J., Moriguchi, T. (2008) Enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis. Phytochemistry, 69(11): 2133-2141.
45. [45] Borell, A., Carbonell, L., Ferras, R., Puig-Parellada, P., Tiburcio, AF. (1997) Polyamines inhibit lipid peroxidation in senescing oat leaves. Physiologia Plantarum, 99(3): 385-390.
46. [46] Yiu, JC., Juang, LD., Fang, DYT., Liu, CW., Wu, SJ. (2009) Exogenous putrescine reduces flooding-induced oxidative damage by increasing the antioxidant properties of Welsh onion. Scientia Horticulturae, 120(3): 306-314.
47. [47] Sinska, I., Lewandawska, U., (1991) Polyamines and ethylene in the removal of embryonal dormancy in apple seeds. Physiologia Plantarum, 81(1): 59-64.