Chemical Profile of Ficus carica L. Leaf Methanol Extract And Its Effect On In Vitro Wound Healing

Öz

Ficus carica L. is among the earliest known cultivated plants of the family Moraceae. It constitutes one of the most important angiosperm genera, encompassing more than 800 species of shrubs, epiphytes, and trees that have been planted in several tropical and subtropical regions across the globe. Ficus carica (FC) leaves have been used not only for tea but also for traditional medicine purposes throughout history. In study, the content of Ficus carica leaf methanol extract (M-FCL) and its proliferation and cell viability properties in human dermal fibroblast (HDF) cell culture were examined. In the first stage of the study, different components in the structure of the extract were identified using gas chromatography-mass spectrometry (GC-MS) and secondly, HDF cell viability (WST-8) and wound healing activity were evaluated. HDF cell viability (WST-8) and wound healing activity were evaluated. To evaluate the wound healing effects of the extract, microscopic images were taken at three-hour intervals (0, 3, 6, 9, 12 and 15 hours) until the wound was closed in any group. Then, they were analyzed with Image J program. Fourteen chemical components were identified in the M-FCL extract by means of GC-MS. Analysis reveals that the main constituent of M-FCL is α-tocopherol (28.181 %). As a result of WST-8 analysis, it was observed that cell viability increased significantly (p< 0.01) at concentrations between 31.25-250 µg/mL. It was observed that M-FCL accelerated wound healing at low concentrations.

Anahtar Kelimeler

• Ficus carica L.
• Gas Chromatography-Mass Spectrometry
• Cell proliferation
• Wound healing
• HDF

Ficus carica L. Yaprak Metanol Ekstraktının Kimyasal Profili ve İn Vitro Yara İyileşmesi Üzerindeki Etkisi

Öz

Öz Ficus carica (Fig.) L., Moraceae familyasının bilinen en eski kültür bitkileri arasındadır. Dünyanın çeşitli tropikal ve subtropikal bölgelerinde ekilen 800'den fazla çalı, epifit ve ağaç türünü kapsayan en önemli angiosperm cinslerinden birini oluşturur. Ficus carica (FC) yaprakları sadece çay olarak değil, aynı zamanda tarih boyunca geleneksel tıp amaçları için de kullanılmıştır. Çalışmada, Ficus carica yaprak metanol özütünün (M-FCL) içeriği ve insan dermal fibroblast (HDF) hücre kültüründe çoğalma ve hücre canlılığı özellikleri incelenmiştir. İyileştirme aşamasında, özütün yapısındaki farklı bileşenler gaz kromatografisi-kütle spektrometrisi (GC-MS) kullanılarak tanımlanmış ve ikinci olarak HDF hücre canlılığı (WST-8) ve yara iyileştirme aktivitesi değerlendirilmiştir. HDF hücre canlılığı (WST-8) ve yara iyileştirme aktivitesi değerlendirilmiştir. Ekstraktın yara iyileştirme etkilerini değerlendirmek için, herhangi bir grupta yara kapatılıncaya kadar üç saatlik aralıklarla (0, 3, 6, 9, 12 ve 15.saatler) mikroskobik görüntüler alınmıştır. Daha sonra, Image J programı ile analiz edilmiştir. M-FCL ekstraktında GC-MS aracılığıyla on dört kimyasal bileşen belirlendi. Analiz, M-FCL'nin ana bileşeninin α-tokoferol (%28.181) olduğunu ortaya koymaktadır. WST-8 analizi sonucunda, hücre canlılığının 31.25-250 µg/mL arasındaki konsantrasyonlarda önemli ölçüde (P <0.01) arttığı görüldü. M-FCL'nin düşük konsantrasyonlarda yara iyileşmesini hızlandırdığı görüldü.

Anahtar Kelimeler

• Ficus carica L.
• Gaz Kromatografisi-Kütle Spektrometrisi
• Hücre çoğalması
• HDF
• Yara iyileşmesi

Kaynakça

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