Biological Activities of Two Stacys Species: Stachys thirkei K. Koch and Stachys macrantha (K. Kock) Stearn

Öz

The study aimed to investigate the antioxidant activities of methanol extracts from Stachys thirkei and Stachys macrantha growing in Eastern Anatolia using the DPPH and ABTS methods, as well as their cytotoxic effects on BPH, DU-145, PC-3, and LNCaP cell lines by MTT assay. Radical scavenging activities were determined spectrophotometrically, revealing that both species exhibited high antioxidant capacity; however, S. thirkei demonstrated relatively higher activity. In the preliminary investigation of potential treatments for prostate cancer, neither species affected the viability of the BPH, DU-145, or PC-3 cell lines. Only the S. thirkei extract exhibited a cytotoxic effect on LNCaP cell lines, with an IC50 value of 19.25 µg/mL.

Anahtar Kelimeler

• Stachys thirkei
• Stachys macrantha
• antioxidant activity
• cytotoxicity

Biological Activities of Two Stacys Species: Stachys thirkei K. Koch and Stachys macrantha (K. Kock) Stearn

Öz

Öz Çalışmanın amacı, Doğu Anadolu’da yetişen Stachys thirkei ve Stachys macrantha bitkilerinin metanol ekstrelerinin antioksidan aktivitelerini DPPH ve ABTS yöntemleri kullanarak, BPH, DU-145, PC-3 ve LNCaP hücre hatları üzerindeki sitotoksik etkilerini de MTT analizi ile araştırmaktır. Radikal süpürücü aktiviteler spektrofotometrik olarak belirlenmiş ve her iki türün de yüksek antioksidan kapasiteye sahip olduğu ortaya çıkmıştır; ancak S. thirkei örneği nispeten daha yüksek aktivite göstermiştir. Prostat kanseri için potansiyel tedavilerin ön araştırmasında, iki tür de BPH, DU-145 veya PC-3 hücre hatlarının canlılığını etkilememiştir. Sadece S. thirkei ekstresinin LNCaP hücre hatları üzerinde IC50: 19,25 µg/mL değeriyle sitotoksisik etkiye sahip olduğu görülmüştür.

Anahtar Kelimeler

• Stachys thirkei
• Stachys macrantha
• antioksidan aktivite
• sitotoksisite

Kaynakça

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