Heterosiklik Benzimidazol–Tiyoeter Türevlerinin İnsan Deri Fibroblast Hücrelerinin Çoğalması Üzerindeki Etkisi

Öz

Benzimidazolün, farmakolojik ve fizikokimyasal özelliklerinden dolayı antikanser çalışmalarında önemli bir yer tutmasına rağmen normal hücre hatlarıda sitotoksik ve güvenlik profillerine ilişkin veriler sınırlı kalmaktadır. Bu sebeple bu çalışmada heterosiklik benzimidazol-tiyoeter bileşiklerinin İnsan deri Fibroblast (HDFa) hücre hattının poliferasyonuna etkisi 10 nM, 100 nM, 500 nM, 1 µM konsantasyonlarda ve 24 ile 48 saat süre ile incelenmiştir. Heterosiklik benzimidazol-tiyoeter moleküllerinden 11 ve 12 için IC50 değerleri 24 ve 48 saat için sırasıyla; 117.93, 13.2 nM ile 52.14, 28.2 nM olarak bulunmuştur. Çalışmada heterosiklik benzimidazol-tiyoeter bileşik-11 için kontrol grubuna en yakın canlılık gösteren konsantrasyonlarda 24 ve 48 saatte 10 nM olarak bulunmuştur. Ayrıca bileşik-11 için 24. saatte 500 nM ve 1 µM konstrasyonları istatiksel olarak anlamlı bulunurken; bileşik-12 için ise 24 saatte 100 nM 500 nM ve 1 µM konstrasyonları istatiksel olarak anlamlı bulunurken 48. Saatte bütün konsantrasyonlar istatiksel olarak anlamlı bulunmuştur (p<0.05).

Anahtar Kelimeler

• Heterosiklik benzimidazol
• insan dermal fibroblastı
• hücre poliferasyonu

Impact of Heterocyclic Benzimidazole–Thioether Derivatives on the Proliferation of Human Dermal Fibroblast

Öz

Benzimidazole derivatives have attracted considerable attention in anticancer research due to their favorable pharmacological and physicochemical properties. However, data regarding their cytotoxicity and safety in normal (non-cancerous) cell lines are still limited. Therefore, in this study, the effect of heterocyclic benzimidazole-thioether compounds on the proliferation of Human Skin Fibroblast (HDFa) cell line was investigated at concentrations of 10 nM, 100 nM, 500 nM, 1 µM and for 24 and 48 hours. IC50 values for heterocyclic benzimidazole-thioether compounds 11 and 12 were found to be 117.93, 13.2 nM and 52.14, 28.2 nM for 24 and 48 hours, respectively. In the study, the closest viability concentrations to the control group for the 11 molecule among the heterocyclic benzimidazole-thioether compounds were found to be 10nm at 24 and 48 hours. In addition, while 500 nm and 1 µM were statistically significant at 24 hours for compound-11, 100 nm, 500 nm and 1 µM were statistically significant at 24 hours for compound-12, while all concentrations were statistically significant at 48 hours (p<0.05).

Anahtar Kelimeler

• Heterocyclic benzimidazole
• human dermal fibroblast
• cell proliferation

Destekleyen Kurum

No funding was received for this study.

Etik Beyan

There are no ethical issues regarding the publication of this study

Kaynakça

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