Crambe orientalis var. orientalis (Brassicaceae) Türünün Farklı Kısımlarının Karşılaştırmalı Antimikrobiyal Aktivitesi

Öz

Bu çalışmada, Crambe orientalis var. orientalis bitkisinin antimikrobiyal potansiyeli meyvelerinden elde edilen yağ ile farklı kısımlarından elde edilen (çiçeklerinden, yapraklarından, köklerinden, meyvelerinden ve toprak üstü kısımlarından hazırlanan) metanolik ve sulu ekstrelerin karşılaştırmalı analizi ile belirlenmiştir. Bitki kısımları maserasyon ve dekoksiyon yöntemleri kullanılarak ekstre edilmiş ve verimleri karşılaştırılmıştır. Ekstre verimi metanol ekstrelerinde genellikle daha yüksek bulunmuş ve bitki kısımlarından toprak üstü kısımlarının verim açısından daha zengin olduğu tespit edilmiştir. Antimikrobiyal aktivite, mikrodilüsyon yöntemi kullanılarak Escherichia coli, Staphylococcus aureus, Candida albicans ve C. tropicalis'e karşı minimum inhibitör konsantrasyonları (MIC) hesaplanarak belirlenmiştir. Çiçeklerin ve toprak üstü kısımların metanol ekstresi, özellikle S. aureus ve E. coli'ye karşı (MIC = 625 µg/mL) belirgin antibakteriyel aktivite göstermiştir. Yaprak ekstreleri ayrıca C. albicans'a karşı antifungal aktivite göstermiştir. C.orientalis’den elde edilen yağ ise S. aureus'a karşı en güçlü aktiviteyi göstermiştir. Sonuçlar, C. orientalis var. orientalis’in doğal antimikrobiyal ajanlar için umut vadeden bir kaynak olduğunu özellikle de yaprakların ve toprak üstü kısımların biyoktivite bakımından öne çıktığını göstermiştir.

Anahtar Kelimeler

• Antimikrobiyal aktivite
• Brassicaceae
• Crambe orientalis var. orientalis
• Mimimum inhibitor aktivite (MIC)

Comparative Antimicrobial Activity of Different Plant Parts of Crambe orientalis var. orientalis (Brassicaceae)

Öz

In this study, the antimicrobial potential of Crambe orientalis var. orientalis was determined by comparative analysis of the fatty oil obtained from its fruits and methanolic and aqueous extracts obtained from different parts of the plant (flowers, leaves, roots, fruits, and aerial parts). Plant materials were extracted using maceration and decoction methods, and their yields were compared. Methanol extracts generally yielded higher amounts, with the aerial parts being the richest. Antimicrobial activity was evaluated against Escherichia coli, Staphylococcus aureus, Candida albicans, and C. tropicalis using the microdilution method to determine minimum inhibitory concentrations (MIC). The methanol extract of the flowers and aerial parts exhibited notable antibacterial activity, particularly against S. aureus and E. coli (MIC = 625 µg/mL). Leaf extracts also showed antifungal activity against C. albicans. The fatty oil demonstrated the strongest activity against S. aureus. The results showed that C. orientalis var. orientalis is a promising source for natural antimicrobial agents, with the leaves and aerial parts standing out in terms of bioactivity.

Anahtar Kelimeler

• Antimicrobial activity
• Brassicaceae
• Crambe orientalis var. orientalis
• Minimum inhibitory concentration (MIC)

Kaynakça

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