Effect of Tianeptine on Oxidative Renal Damage Induced by Ischemia Reperfusion in Rats

Yıl 2018, Cilt: 11 Sayı: 2, 300 - 305, 31.08.2018

Bahadir Süleyman , Aslı Özbek Bilgin , Renad Mammadov

https://doi.org/10.18185/erzifbed.422543

Cited By: 2

https://izlik.org/JA22DA52HU

Öz

Renal ischemia-reperfusion (I/R) damage can develop following
major surgical conditions such as shock, trauma, and renal transplantation and
causes acute renal failure. Kidney I/R damage is known to be a pathological
process that begins with the touch free of oxygen and continues with the
production of free oxygen radicals. We will investigate the effect of renal I/R
injury on tianeptine, an atypical antidepressant drug with antioxidant
properties. There was no information on the effect of tianeptine on I/R-induced
kidney damage in the literature. The aim of the
present study was to investigate the effect of tianeptine on oxidative kidney
damage induced by I/R in rats. Albino wistar male rats were divided into groups
as renal I/R (RIR) group, 25 mg/kg of thianeptine+renal I/R (TIR-25) group and
sham operation (SG) group. The I/R procedure (one hour ischemia, three hours
reperfusion) was performed with 25 mg/kg intraperitoneal thiopental sodium
anesthesia. Oxidative damage was assessed by malondialdehyde (MDA) and total
glutathione (TGSH) measurements in kidney tissue. Biochemical test results
showed that the I/R process increased the amount of MDA in the kidney tissue
and decreased the tGSH. Tianeptine significantly prevented the increase of MDA
and decrease of tGSH in kidney tissue after I/R. Our experimental results suggest
that tianeptine may be useful in preventing I/R-induced oxidative renal injury.

Anahtar Kelimeler

Tianeptine , Ischemia/Reperfusion , Kidney , Malondialdehyde , Total glutathione

Tianeptinin Sıçanlarda İskemi Reperfüzyonla Oluşturulan Oksidatif Böbrek Hasarına Etkisi

https://izlik.org/JA22DA52HU

Öz

Böbrek iskemi-reperfüzyon (İ/R) hasarı  şok, travma
ve böbrek transplantasyonu gibi majör cerrahi durumları takiben
gelişebilmektedir ve akut böbrek yetmezliğine neden
olmaktadır. Böbrek İ/R hasarının,   dokunun
oksijensiz  kalması ile başlayan ve  serbest oksijen
radikallerinin üretimi ile devam eden patolojik bir süreç olduğu
bilinmektedir. Çalışmamızda böbrek İ/R hasarına etkisini araştıracağımız
tianeptin,  antioksidan özelliğe sahip atipik antidepresan bir
ilaçtır. Literetürlerde tianeptinin, İ/R ile oluşturulan böbrek hasarına
karşı etkisine ait bilgilere rastlanmadı. Çalışmamızın amacı tianeptinin, sıçanlarda İ/R ile oluşturulan
oksidatif böbrek hasarına etkisini araştırmaktır. Albino wistar türü erkek
sıçanlar renal iskemi-reperfüzyon (RİR), 25 mg/kg tianeptin+renal
iskemi-reperfüzyon (TİR-25) ve şam operasyonu uygulanacak (SG)
gruplara  ayrıldı. İ/R işlemi (bir saat iskemi, üç saat reperfüzyon),
25 mg/kg intraperitoneal tiopental sodyum anestezisi uygulanarak yapıldı.
Oksidatif hasar böbrek dokusunda malondialdehid (MDA) ve total glutatyon (tGSH)
ölçümü ile değerlendirildi. Biyokimyasal deney sonuçları, İ/R işleminin böbrek
dokusunda MDA miktarının artmasına tGSH’nın ise azalmasına neden olduğunu
göstermiştir. Tianeptinin, böbrek dokusunda MDA’nın İ/R ile artmasını ve
tGSH’nın azalmasını anlamlı önlediği saptanmıştır. Deney
sonuçlarımız, tianeptinin İ/R ile indüklenen oksidatif böbrek hasarını
önlemede yararlı olabileceğini düşündürmektedir.

Anahtar Kelimeler

Malondialdehid , Tianeptin , İskemi/Reperfüzyon , Böbrek , Total glutatyon

Kaynakça

• Abou, H.H.O., Atef, H., Said, E., Elkashef, H.A., Salem, H.A. 2018. Crocin reverses unilateral renal ischemia reperfusion injury-induced augmentation of oxidative stress and toll like receptor-4 activity. Environmental Toxicology and Pharmacology, 59(4), 182-189.
• Alataş, Ö., Şahin, A., Çolak, Ö., Inal, M., Köken, T., Yaşar, B., Karahüseyinğlu, E. 1996. Beneficial effects of allopurinol on glutathione levels and glutathione peroxidase activity in rat ischaemic acute renal failure. Journal of International Medical Research, 24(1), 33-39.
• Altuner, D., Cetin, N., Suleyman, B., Aslan, Z., Hacimuftuoglu, A., Gulaboglu, M., Suleyman, H. 2013. Effect of thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat kidney. Indian Journal of Pharmacology, 45(4), 339.
• András, S. 2008. " Atypical" antidepressive mechanisms: glutamatergic modulation and neuroplasticity in case of tianeptine. Neuropsychopharmacologia Hungarica, 10(5), 305-313.
• Baron, P., Gomez, M.O., Casas, C., Heil, J., Will, N., Condie, R., Sutherland, D. E. 1991. Renal preservation after warm ischemia using oxygen free radical scavengers to prevent reperfusion injury. Journal of Surgical Research, 51(1), 60-65.
• Cetin, N., Suleyman, H., Sener, E., Demirci, E., Gundogdu, C., Akcay, F. 2014. The prevention of ischemia/reperfusion induced oxidative damage by venous blood in rabbit kidneys monitored with biochemical, histopatological and immunohistochemical analysis. Journal of Physiology and Pharmacology, 65(3), 383-392.
• Choucry, M.A., Khalil, M.N.A., El Awdan, S.A. 2018. Protective action of Crateva nurvala Buch. Ham extracts against renal ischaemia reperfusion injury in rats via antioxidant and anti-inflammatory activities. Journal of Ethnopharmacology, 214(5), 47-57.
• Del, R.F.M. 1980. An approach to free radicals in medicine and biology. Acta Physiologica Scandinavica. Supplementum, 492(1), 153-168.
• Douzinas, E.E., Livaditi, O., Tasoulis, M.K., Prigouris, P., Bakos, D., Goutas, N., Tsoukalas, G.D. 2012. Nitrosative and oxidative stresses contribute to post-ischemic liver injury following severe hemorrhagic shock: the role of hypoxemic resuscitation. PLoS One, 7(3), 329-68.
• Harman, D. 2001. Aging: overview. Annals of the New York Academy of Sciences, 928(1), 1-21.
• Hermes, L.M., Zenteno, S.T. 2002. Animal response to drastic changes in oxygen availability and physiological oxidative stress. Comparative Biochemistry and Physiology Part C: Toxicology Pharmacology, 133(4), 537-556.
• Karaca, I., Delibas, I.B., Yapca, O.E., Ingec, M., Kumbasar, S., Hirik, E. Bilgin, A.O. 2017. Oxidative Renal Damage in Rats Subjected to Ovarian Ischemia Reperfusion and Protective Effect of Tianeptine. Latin American Journal of Pharmacy, 36(12), 2339-2347.
• Larson, R.A. 1988. The antioxidants of higher plants. Phytochemistry, 27(4), 969-978.
• Lien, Y.H.H., Lai, L.W., Silva, A.L. 2003. Pathogenesis of renal ischemia/reperfusion injury: lessons from knockout mice. Life sciences, 74(5), 543-552.
• Liu, Z.G., Qi, Z.C., Liu, W.L., Wang, W.Z. 2015. Lutein protects against ischemia/reperfusion injury in rat kidneys. Molecular Medicine Reports, 11(3), 2179-2184.
• Meister, A., Anderson, M.E. 1983. Glutathione. Annual Review of Biochemistry, 52(1), 711-760.
• Mitchell, B.J., Russo, A. 1987. The role of glutathione in radiation and drug induced cytotoxicity. The British Journal of Cancer Supplement, 55(8), 96-104.
• Ohkawa, H., Ohishi, N., Yagi, K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95(2), 351-358.
• Reeta, K., Prabhakar, P., Gupta, Y.K. 2016. Anticonvulsant activity of the antidepressant drug, tianeptine, against pentylenetetrazole-induced seizures mitigates cognitive impairment in rats. Behavioural Pharmacology, 27(7), 623-632.
• Sedlak, J., Lindsay, R.H. 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical Biochemistry, 25(1), 192-205.
• Suleyman, H., Cadirci, E., Albayrak, A., Polat, B., Halici, Z., Koc, F., Bayir, Y. 2009. Comparative study on the gastroprotective potential of some antidepressants in indomethacin-induced ulcer in rats. Chemico-Biological Interactions, 180(2), 318-324.
• Şener, G., Tuğtepe, H., Yüksel, M., Çetinel, Ş., Gedik, N., Yeğen, B.Ç. 2006. Resveratrol improves ischemia/reperfusion-induced oxidative renal injury in rats. Archives of Medical Research, 37(7), 822-829.
• Torres, G.L., Cienfuegos, P.E., Perales, Q.M.M., Alarcon, G.G., Muñoz, E.L.E., Pérez, R.E., Cordero, P.P. 2018. Nephroprotective Effect of Sonchus oleraceus Extract against Kidney Injury Induced by Ischemia-Reperfusion in Wistar Rats. Oxidative Medicine and Cellular Longevity, Article ID 9572803, 7p.
• Toyokuni, S. 1999. Reactive oxygen species‐induced molecular damage and its application in pathology. Pathology International, 49(2), 91-102.
• Young, I., Woodside, J. 2001. Antioxidants in health and disease. Journal of Clinical Pathology, 54(3), 176-186.