Entaurea kurdica Reichardt and Centaurea urvillei DC. subsp. hayekiana Wagenitz’nın Element İçeriği ve Antioksidan Enzimlerinin Belirlenmesi

Öz

Kronik hastalıklar dünya çapında hızla artmaktadır. Tüm yaşam boyunca sağlığın iyileştirilmesi ve sürdürülmesinde beslenme ve diyet önemli bir yere sahiptir. Organizmadaki biyokimyasal ve fizyolojik değişim, biyomoleküllerde oksidatif hasara yol açan serbest radikallerin aşırı artmasına neden olabilir. Son zamanlarda tıbbi bitkilerin kullanımı antioksidanlar, antikanser ve hipoglisemik aktiviteler gibi yararlı özellikler nedeniyle artmıştır. Bu çalışmada kullanılan, Centaurea kurdicaReichardt ve Centaurea urvillei DC. subsp. hayekiana Wagenitz (Asteraceae) Elazığ'ın çeşitli bölgelerinde doğal olarak yetişen endemik türlerdir. Centaurea'nın birçok taksonu geleneksel olarak hem ülkemizde hem de dünyada tıbbi amaçlarla ve besinler için kullanılmaktadır. Centaurea kurdica Reichardt ve Centaurea urvilleiDC. subsp. hayekianaWagenitz (Asteraceae) taksonları Elazığ'daki doğal yaşam alanlarından elde edilmiştir. Bitki dokularında ağır metal akümülasyonu, antioksidan enzimler süperoksit dismutaz (SOD), glutatyon S transferaz (GST), askorbat peroksidaz (AP) ve katalaz (CAT) aktiviteleri gibi fizyolojik değişiklikler tespit edilmiştir. Çalışılan enzimlerden SOD en fazla aktiviteye sahip olan enzimdir. Yapılan çalışma sonucunda en fazla akümülasyonun genellikle yapraklarda olduğu tespit edilmiştir. Antioksidan enzim aktiviteleri: süperoksit dismutaz (SOD), glutatyon S transferaz (GST), askorbat peroksidaz (APX) ve katalaz (CAT) değerlendirildi Centaurea kurdica'da sırasıyla 1.315, 0.011, 0.031 ve 0.007 unit mg^-1olarak tespit edildi. C. kurdica'nın Centaurea urvilleiDC. subsp. hayekiana’dan askorbat peroksidaz dışında değerlendirilen tüm enzim aktivitelerinde daha iyi olduğu belirlenmiştir.

Anahtar Kelimeler

• Anahtar Kelimeler: Centaurea kurdica Reichardt,Centaurea urvillei DC. subsp. hayekiana Wagenitz,endemik,antioksidan enzim

Evaluation of antioxidant enzymes and elements content of Centaurea kurdica Reichardt and Centaurea urvillei DC. subsp. hayekiana Wagenitz

Öz

Chronic illnesses are quickly increasing worldwide. Nourishment and diet have an important place in the improvement and maintenance of health throughout the whole life. Biochemical and physiological change in the organism may result in overexpress of free radicals causing to oxidative injury to biomolecules. Recently, the use of medical plants has increased due to their useful properties such as antioxidants, anticancer and hypoglycaemic activities. Centaurea kurdicaReichardt and Centaurea urvilleiDC. subsp. hayekianaWagenitz (Asteraceae)  used in this study taxa are endemic species naturally grown in various regions of Elazig. Many taxa of Centaureaare being traditionally used both in our country and across the world for medical purposes as well as nutrients. Centaurea kurdicaReichardt and Centaurea urvilleiDC. subsp. hayekianaWagenitz (Asteraceae) taxa were obtained from their natural habitats in Elazığ. Physiological changes such as heavy metal segregation, antioxidant enzymes superoxide dismutase (SOD), glutathione S transferase (GST), ascorbat peroxidase (AP) and catalase (CAT) activities in plant tissues were determined. Among the enzymes studied, SOD is the enzyme with the highest activity. It has been determined that the maximum accumulation of the accumulation is usually on the leaves. The activities of enzymatic antioxidants: superoxide dismutase (SOD), glutathione S transferase (GST), ascorbat peroxidase (APX) and catalase (CAT) activities were assayed and found significantly higher i.e. 1.315, 0.011, 0.031 and 0.007 (unit mg^-1fresh tissues) respectively in Centaurea kurdicathan Centaurea urvilleiDC. subsp. hayekiana, where C. kurdicawas recorded next plant in order to be better-off in the entire enzyme activity assessed except ascorbate peroxidase.

Anahtar Kelimeler

• Centaurea kurdica Reichardt,Centaurea urvillei DC. subsp. hayekiana Wagenitz,endemic,antioxidant enzyme

Kaynakça

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