Evaluation of Retrotransposon Mobility Caused by Growth Regulator 2,4-D Isooctylester Herbicide in Wheat (Triticum aestivum L.) Seeds by Molecular Method

Öz

The aim of this study was to investigate the effects of herbicides on retrotransposon activity using IRAP (Inter Retrotransposon Amplified Polymorphism) technique. For this purpose, For this purpose, 2,4-D Isooctylester was applied on wheat seeds with increasing doses (0, 0.1, 0.2, 0.3, 0.4 and 0.5 ppm). Six primers were used for IRAP analysis. As a result of the study, it was observed that retrotransposon polymorphism was high (15.38% -53.84%) in 2,4-D Isooctylester application. As a result of the increase in DNA damage, the rate of GTS (Genomic Stability Stability) decreased (84.62% and 46.16%). The results showed that IRAP method can be used effectively to examine the effect of stress factors on retrotransposon mobility.

Buğday (Triticum aestivum L.) Tohumlarında Büyüme Düzenleyicisi 2,4-D Isooctylester Herbisitinin Meydana Getirdiği Retrotranspozon Hareketliliğinin Moleküler Yöntem İle Değerlendirilmesi

Öz

Bu çalışmanın amacı, herbisitlerin retrotranspozon aktiviteleri üzerindeki etkilerini IRAP (Retrotranspozon-arası çoğaltılmış polimorfizm) tekniği kullanarak araştırmaktı. Bu amaçla buğday tohumlarına çimlenme aşamasında artan dozlarda (0, 0,1, 0,2, 0,3, 0,4 ve 0,5 ppm) 2,4-D Isooctylester uygulanmıştır. IRAP analizleri için altı primer kullanılmıştır. Çalışma sonucunda, 2,4-D Isooctylester uygulamasında retrotranspozon polimorfizminin yüksek olduğu (%15.38 - %53.84) gözlemlenmiştir. Doz artışına bağlı olarak DNA hasarı sonucu GTS (Genomik Kararlılık Sabitliği) oranının (%84.62 ve %46.16) azaldığı görülmüştür. Sonuçlar bize stres faktörlerinin retrotranspozon hareketliliğine etkisinin incelenmesinde IRAP yönteminin etkin bir şekilde kullanılabileceğini göstermiştir.

Anahtar Kelimeler

• Herbisit,Retrotranspozon-arası çoğaltılmış polimorfizm,Retrotranspozon,Triticum aestivum L.

Kaynakça

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