Ihlamur Ekstraktı Kullanılarak Yeşil Sentez ile Manyetik Antibiyotik Gönderim Sisteminin Hazırlanması ve Karakterizasyonu

Öz

Yeşil kimya, kolay, ucuz ve çevre dostu yöntemlerle demir oksit nanopartiküllerin üretimini mümkün kılar. Amoksisilin çeşitli bakteri enfeksiyonlarının tedavisinde kullanılmakla birlikte antibiyotik direnci tedavi için büyük bir problemdir. Demir oksit nanopartikülleri gerek bu problemlerin üstesinden gelinmesi için gerekse antibiyotiklerin manyetik hedeflendirilmesi için önde gelen alternatiflerden biridir. Bu çalışmada ıhlamur ekstraktı kullanılarak yeşil kimya temelli demir oksit nanopartiküllerinin sentezlenmesi ve hedefli antibiyotik taşınım sisteminin oluşturulması için bu nanopartiküllere amoksisilin yüklenmesi amaçlanmıştır. Demir oksit nanopartikülleri sulu ıhlamur ekstraktı ve FeCl3 çözeltisi kullanılarak hazırlandı. Bu yapılar FTIR, SEM ve zetasizer ile karakterize edildi. İlaç adsropsiyonu için değişen konsantrasyonlarda amoksisilin çözeltisi manyetik nanopartiküller ile inkübe edildi ve pH 7,4 ortamında ilaç salım çalışması yapıldı. Nanopartiküllerin küresel morfolojiye (70-88 nm) ve demir oksit yapısına sahip olduğu ayrıca polifenoller ile sarılı halde olduğu belirlendi. Nanopartiküllere yüklenen optimum amoksisilin miktarı 100.17 μg ilaç/ mg nanopartikül olarak tespit edildi. İlaç salımının ilk saatlerde daha hızlı ardından kontrollü bir şekilde devam ettiği gözlendi. Düşük maliyet ve çevre dostu üretimle elde edilen amoksisilin yüklü demir oksit nanopartiküllerin bakteriyel enfeksiyon hastalıklarının manyetik hedefli tedavisinde potansiyel taşıdığı düşünülmektedir.

Anahtar Kelimeler

• manyetik nanopartikül
• amoksisilin
• yeşil kimya
• ıhlamur
• hedefli antibiyotik gönderimi

Green Synthesis and Characterization of Magnetic Antibiotic Delivery System Using Linden Extract

Öz

Green chemistry enables iron oxide nanoparticle production with easy, cost-effective and environmentally friendly way. Amoxicillin is used in the treatment of various bacterial infections, however antibiotic resistance is a major problem for treatment. Iron oxide nanoparticles are promising alternatives for overcoming these problems and magnetically targeted antibiotic delivery. In this work, it was aimed to synthesis of iron oxide nanoparticles with green chemistry method utilizing the linden plant, and loading of amoxicillin to these magnetic nanoparticles to be targeted antibiotic delivery system. Iron oxide nanoparticles were prepared using aqueous linden extract and FeCl3 solution. These nanostructures were characterized with FTIR, SEM, and zetasizer. Amoxicillin solutions at varying concentrations were incubated with magnetic nanoparticles for drug adsorption and in vitro drug release from nanoparticles at pH 7.4 was studied. It was found that nanoparticles had spherical morphology (70-88 nm), iron oxide structure and were capped with polyphenols. Optimum amoxicillin amount in magnetic nanoparticles was determined as 100.17 μg drug/ mg nanoparticle. Drug release was faster in the first hours and proceeded in controlled manner. It can be suggested that amoxicillin loaded iron oxide nanoparticles could have potential of magnetically targeted therapy of bacterial infectious disease with low cost and ecofriendly production.

Anahtar Kelimeler

• magnetic nanoparticle
• amoxicillin
• green chemistry, linden, targeted antibiotic delivery

Kaynakça

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