Ferulago pauciradiata Boiss. & Heldr. Effects of Prantschimgin Compound Isolated from the Root of the Plant on LPS-Induced Inflammation Model in the L929 Cell Line

Öz

L929 hücre hattında oluşturulan lipopolisakkarit (LPS) kaynaklı inflamasyon modelinde Ferulago pauciradiata (Apiaceae) bitkisinin kök kısmından izole edilen prantşimgin (Prn) bileşiğinin etkilerinin incelenmesi. L929 hücre hatları, 37°C'de %5 CO2'li inkübatörde standart hücre kültürü prosedürleri kullanılarak son konsantrasyonu 2 µL olacak şekilde Prn saf maddesi ve 1 saat sonra son konsantrasyonu 1 µL olacak şekilde LPS uygulandı. LPS uygulamasının ardından, gerekli olan 24, 48 ve 72 saat inkübasyon süreleri sonunda sitotoksik etkilerin belirlenmesi amacıyla kolorimetrik bir metot olan MTT protokolü uygulandı. IC50 değerleri, Prn için 0.28 µg/mL ve LPS için ise 1 µg/mL olarak hesaplandı. L929 hücre hatlarına LPS uygulanmasının zamana bağlı olarak hücresel indekste anlamlı derecede azalmaya neden olmuştur. Ayrıca Prn+LPS gruplarında ise sadece LPS uygulanan grubuna göre azalmış hücre indeksini, anlamlı derecede artırdığı hatta kontrole yaklaştırdığı tespit edilmiştir.L929 hücre hattına uygulana LPS ile oluşturulan inflamasyonun ve hücre hasarını Pnr uygulaması sonucunda düzeldiği tespit edildi.

Anahtar Kelimeler

• Ferulago pauciradiata
• inflammation
• L929
• LPS
• MTT
• prantschimgin

Ferulago pauciradiata Boiss. & Heldr. Effects of Prantschimgin Compound Isolated from the Root of the Plant on LPS-Induced Inflammation Model in the L929 Cell Line

Öz

Investigation of the effects of prantchimgin (Prn) compound isolated from the root part of the Ferulago pauciradiata (Apiaceae) plant in the lipopolysaccharide (LPS) cytotoxicity model created in the L929 cell line. L929 cell lines were applied in a 5% CO2 incubator at 37 °C, using standard cell culture procedures, LPS was applied with Prn pure substance with a final concentration of 2 µL and 1 µL after 1 hour. Following the LPS application, the MTT protocol, a colorimetric method, was applied to determine cell viability at the end of the required 24th, 48th, and 72nd hours incubation times. IC50 values were calculated as 0.28 µg/mL for Pnr and 1 µg/mL for LPS. Application of LPS to L929 cell lines caused a significant decrease in cellular index depending on time. Also, in the Prn + LPS groups, it was found that the decreased cell index significantly increased even closer to the control compared to the LPS applied group. It was found that cyctoxicity and cell damage caused by LPS applied to the L929 cell line improved after Pnr application.

Anahtar Kelimeler

• ferulago pauciradiata
• cyctoxicity
• L929
• LPS
• MTT
• prantschimgin

Kaynakça

1. References Bistrian B. 2007. Systemic response to inflammation. Nutrition Reviews, 65,170–172
2. Chertov O., Yang D., Howard O. 2000. Leukocyte granule proteins mobilize innate host defenses and adaptive immune responses. Immunological Reviews, 177,68–70.
3. Chunxiao W., Congcong Z., Luxin, L. 2017. Macrophage-derived mir-155-containing exosomes suppress fibroblast proliferation and promote fibroblast inflammation during cardiac injury. Molecular Threapy, 25(1), 192-204.
4. De Lima F.O., Fabiana R.N., Ricardo D.C. 2011. Mechanisms involved in the antinociceptive effects of 7-hydroxycoumarin. Journal of Natural Products, 74(4), 596-602.
5. Dinarello C.A. 2007. Historical insights into cytokines. European Journal of Immunology. 37(1),34–44.
6. Dinarello CA. 1996. Cytokines as mediators in the pathogenesis of septic shock. Current Topic Microbiology Immunology, 216,133–165.
7. DIN EN ISO 10993-5: Biologische Beurteilung von Medizinprodukten—Teil 5: Prüfungen auf In-vitro-Zytotoxizität (ISO 10993-5:2009); German Version of EN ISO 10993-5:2009; International Organisation for Standardization: Geneva, Switzerland, 2009.
8. Ferrero L., Nielsen O.H., Andersen P.S. 2006. Chronic inflammation: importance of NOD2 and NALP3 in interleukin‐1β generation. Clinical and Experimental Immunology, 147,227-235.

9. Filer A., Raza K., Salmon M., Buckley C.D. 2008. The role of chemokines in leucocyte-stromal interactions in rheumatoid arthritis. Frontiers in bioscience: a journal and virtual library. 13:2674–2685.
10. Fujiu K., Wang J., Nagai R. 2014. Cardioprotective function of cardiac macrophages. Cardiovascular Research 102:232–239.
11. Guha M., Mackman N. 2001. LPS induction of gene expression in human monocytes. Cell Signaling, 13,85–94.
12. Guner A., Aslan S. Türkiye bitkileri listesi:(damarlı bitkiler): Nezahat Gökyiǧit Botanik Bahçesi Yayınları, Istanbul, 2012.
13. Heinrichs D., Knauel M., Offermanns C. 2011. Macrophage migration inhibitory factor (MIF) exerts antifibrotic effects in experimental liver fibrosis via CD74. Proceedings of the National Academy of Sciences, 108:17444–17449.
14. Hoang A.T. Dong-Cheol K., Wonmin K. 2017. Anti-inflammatory coumarins from Paramignya trimera. Pharmaceutical Biology, 55(1), 1195–1201.
15. Iwasaki A., Medzhitov R. 2004. Toll-like receptor control of the adaptive immune responses. Nature Immunology,5, 987–95.
16. Jianjun X., Yongxin Z., Haji A.A. 2017. Anti-inflammatory effect of pomegranate flower in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Pharmaceutıcal Bıology, 55(1),2095–3001.
17. Kasumbwe K., Kabange N., Venugopala N. 2017. Synthetic Mono/di-halogenated Coumarin Derivatives and Their Anticancer Properties. Anti-Cancer Agents in Medicinal Chemistry, 17(2),276-85.
18. Karakaya S., Koca M., Simsek D. 2018. Antioxidant, Antimicrobial and Anticholinesterase Activities of Ferulago pauciradiata Boiss. & Heldr. Growing in Turkey. Journal of Biologically Active Products from Nature, 8(6), 364-375.
19. Kaminska B. 2005. MAPK signalling pathways as molecular targets for anti-inflammatory therapy--from molecular mechanisms to therapeutic benefits. Biochim Biophys Acta, 1754,253–262.
20. Linlin C., Huidan D., Hengmin C., Jing F., Zhicai Z., Junliang D., Yinglun Li., Xun W., Ling Z. 2018. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget, 9 (6),7204–18.
21. McNamara A., Jenne B.M., Dean M.F. 1985. Fibroblasts acquire beta-glucuronidase by direct and indirect transfer during co-culture with macrophages. Experimental Cell Research Research, 160,150–157. Millar N.L., Murrell G.A.C., McInnes I.B. Alarmins in tendinopathy: unravelling new mechanisms in a common disease. Rheumatology (Oxford). 2012;In Press.
22. Mihai G., Frances B., Michel C., 2017. A guiding map for inflammation. Nature Immunology, 18(8), 826-31.
23. Mustafa Y.F., Najem M.A., Tawffiq Z.S. 2018. Coumarins from Creston Apple Seeds: Isolation, Chemical Modification, and Cytotoxicity Study. Journal of Applied Pharmaceutical Science, 8(08), 49-56.
24. Moeed A., Michael M., Emma G.M. 2019. Fibroblast activation and inflammation in frozen shoulder. Plus One, 1-16.
25. Mogensen T.H. 2009. Pathogen recognition and inflammatory signaling in innate immune defenses. Clinical Microbiology Reviews, 22,240–273.
26. Nathan C., Ding A. 2010. Nonresolving inflammation. Cell, 140:871–882.
27. Ruslan M. 2010. Inflammation 2010: New Adventures of an Old Flame. CelPres, 140 (6), 771-776.
28. Sen Z., Jie M., Dongming Z. 2017 Total Coumarins from Hydrangea paniculata Show Renal Protective Effects in Lipopolysaccharide-Induced Acute Kidney Injury via Anti-inflammatory and Antioxidant Activities. Frontiers in Pharmacology, 8:872.
29. Shao J., Li Y., Wang Z. 2013. A novel naphthalimide derivative, exhibited anti-inflammatory effects via targeted-inhibiting TAK1 following down-regulation of ERK1/2- and p38 MAPK-mediated activation of NF-kB in LPS-stimulated RAW264.7 macrophages. International Immunupharmacol, 17, 216–28
30. Shi C., Yuan S., Zhang H. 2009. Cell-mediated immune responses and protective efficacy against infection with mycobacterium tuberculosis induced by Hsp65 and hIL-2 fusion protein in mice. Scandinavian Journal of Immunology, 69, 140–49.
31. Srikrishna D., Godugu C., Pramod K. 2018. A Review on Pharmacological Properties of Coumarins. Mini Reviews in Medicinal Chemistry, 18(2),113-141.
32. Thomas W. Two types of fibroblast drive arthritis. 2019, Nature, 570,169-70.
33. Ulrike N., Sandrine L., Andrea G. 2018. Anti-inflammatory effects of Phaeodactylum tricornutum extracts on human blood mononuclear cells and murine macrophages. Journal of Applied Phycology, 30,2837–2846.
34. Venugopala K.N., Rashmi V., Odhav B. 2013. Review on natural coumarin lead compounds for their pharmacological activity. BioMed Research International,14.
35. Vysakh A., Prasad G., Kuriakose J. 2018. Rotula aquatica Lour attenuates secretion of proinflammatory mediators and cytokines in lipopolysaccharide-induced inflammatory responses in murine RAW 264.7 macrophages. Inflammopharmacology, 26, 29–38.